FastQCFastQC Report
Sat 31 Dec 2022
SRR5843804.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843804.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121984
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTAGACGATCTCGTATGC8810.7222258656873032No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAGACGATCTCGTATGCCGTCT7900.6476259181532005RNA PCR Primer, Index 33 (95% over 21bp)
TGCTTAGGGGCTCAAATACTAGGTAACTATTTTGTTTTAACTTATAAAATGT1290.105751573976915No Hit
TTTCCAGGGAATTTAGCATATTGACAAAGTAATCTGGATTTATACCTTTTAA1280.1049317943336831No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAGACGATCCTGTCTCTTATAC1260.10329223504721931No Hit
TCAATAGGGAGCCCTACTGCATCATCTGCGTCAGCCGGTCCTAGCCCATCTG1240.10165267576075551No Hit
TCGTCGGGGCTATGTGATATGAGTTATCACAATAGGAGGAAAAAGGCTTCCT1220.1000131164742917No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)