FastQCFastQC Report
Sat 31 Dec 2022
SRR5843728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57967
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGTGAATCTTATAGGCACTTTCAATGTGATCCGCCTGGT850.14663515448444805No Hit
AACTACGGGGTCCTCTAAGCGTCACCACGACTTCAACGTCCTGAGT800.13800955716183344No Hit
TTCATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA800.13800955716183344No Hit
CCTATTGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTTG780.13455931823278763No Hit
TGGCAAGGGATCTCAAAACACCTTTCCAAAAGAAGTTGTTGAAAAT770.13283419876826472No Hit
GGCTGCGGGGAACAGAGATGCAGAAAACACAGAGGAACAGATGTGA760.13110907930374177No Hit
CCAAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC750.12938395983921885No Hit
TTCTATGGGATTTCTAAGCTGCATCTCAGTGCCTGGGAGAGCCCTC750.12938395983921885No Hit
GCCACAGGGATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTT740.12765884037469594No Hit
AACTCCGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACATG740.12765884037469594No Hit
ATTAAAGGGCTATAATTCAATTACACTATTAACCCTTGGCCTACTC740.12765884037469594No Hit
GCAATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA730.12593372091017302No Hit
GCACTGGGGATATCTCTCAGACCAGCAATCGTGGTTCCGGAAGCCT720.12420860144565012No Hit
TTACTAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA710.1224834819811272No Hit
TTACTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC710.1224834819811272No Hit
ACTTGCGGGATACAAAAATAAAATATAAACCATAAAATGTACTACC710.1224834819811272No Hit
AGTGAACGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTT700.12075836251660428No Hit
TCTCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA680.11730812358755843No Hit
TACTTAGGGGTTTATTCCCAAGATAACTTTTGTAACTAACTCCTAT680.11730812358755843No Hit
GCATCAGGGCTTCCGATCTAGCCAAGGGGTTTGAATATATTGGTGA670.11558300412303552No Hit
CCCTAAGGGATAAAATTAAAATTAGATGATACTTAAATCTTTCCAG670.11558300412303552No Hit
CTTAACGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC670.11558300412303552No Hit
TGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA670.11558300412303552No Hit
GATACTGGGAGTTCTCATTCACTAGATGTTCAAAGCCACCCTCAGA660.11385788465851261No Hit
CTGCTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA660.11385788465851261No Hit
CGCAAAGGGGATCCGCTTCCGGGGCCACGGCCGAAGTGAAACGCTC660.11385788465851261No Hit
ACTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT660.11385788465851261No Hit
GTATGAAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA650.1121327651939897No Hit
CTGTTTGGGAAGTTCCTGAAGTCTGGCGATGCTGCCATTGTTGATA650.1121327651939897No Hit
ATGTTTGGGCCTGATACAGCAGGCACTCACCATGTTCCCTGGAGTT650.1121327651939897No Hit
GCTTGCGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC650.1121327651939897No Hit
TCTCATGGGAATTTCAAAATACTAGTAAAAGAAAAACCACTGGCTT640.11040764572946676No Hit
TTAGTTGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAAT640.11040764572946676No Hit
ACATGAGGGAATCTCCAGCATATAGCATTTGTGACTGTTACAACAA640.11040764572946676No Hit
TGATTGGGGAGCCTGCGGAACGTCTCCTGCCAGGCACAGATGAGCA640.11040764572946676No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA640.11040764572946676No Hit
AGCACAGGGATTCAGATAATTTTACTCTGCATCACAATGTATTTCC630.10868252626494385No Hit
CTGGGGAGGTCTCCTCACACCGGTCCAGTGGCCACATTTGCAGCGT630.10868252626494385No Hit
ATAACTGGGATGAGCTGGCTAGAGGAAAATGTCCATGAGGTTCTGC630.10868252626494385No Hit
TCAATTGGGGCGGATGATTGATGGGAAGCCAGAATACAAGAATGGG630.10868252626494385No Hit
TCTGCTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG630.10868252626494385No Hit
TTGTATGGGGCATGCGCATTGCGTCCCTTCCTCTTCGGACTTGGGC630.10868252626494385No Hit
TACGCAGGGGGGACGCGGCAGTGGCGACGGCGACGGGCGTTGCGAG630.10868252626494385No Hit
ACAACCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG620.10695740680042093No Hit
CAGCCCCGGCGTGGAGGCAGGTGAATGCGATGGCTACAGGCTCCTT620.10695740680042093No Hit
GACCATGGGGTGTCTAATTTTGATGACGGAAAGGAATTGCCTATTG620.10695740680042093No Hit
GATATAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC620.10695740680042093No Hit
CATAACGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTG610.105232287335898No Hit
GTAACGGGGGAGAAGGTGAAGATGGCTGCCGCCCGGGCTGCGGCCC610.105232287335898No Hit
TTTGGTGGGGGGTCATTGTCCTTGGCATTGGCGTTTACATATACTA610.105232287335898No Hit
TTTCGAGGGAAGACATTAGAAGATCTCAGGAAAAATGAAGATAAAC610.105232287335898No Hit
CCTCCCGGGAAAGTACTTTCAGAATTCAAGGACTAACTTTACTTTT610.105232287335898No Hit
CCGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA610.105232287335898No Hit
ACGTATGGGATGTATATTTTAGCTTGTCATGAAATGTCAACGAAGG610.105232287335898No Hit
TATTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC610.105232287335898No Hit
ACACTTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCC610.105232287335898No Hit
AACAACCGGGGGACTCGAAGCGCTTCTTCTTCGACGTCGGCTGCAA600.10350716787137508No Hit
CAATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA600.10350716787137508No Hit
AATATGGGATCAGTTCCCAGACTGTGATTCCGATGAGGACGAGGAC600.10350716787137508No Hit
TACAAGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA600.10350716787137508No Hit
AGATTTGGGATTCAAATTTATTTGACGTTTTATTTTGGTTTTCCTC600.10350716787137508No Hit
TTCTTAGGGCAGTGAACTGCAATGAGAAGATTGTGGTCCTCCTGCA600.10350716787137508No Hit
CTAGCAGGGAGAGTTGGCAATATTTTTTGATGTACAAAACTTTACA600.10350716787137508No Hit
TTAACCGGGATGGCCCAAAAACTATAGTATAAGTTTGAAATTTCGG590.10178204840685218No Hit
TCCAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC590.10178204840685218No Hit
CTGCTAGGGGCCCCCGCGCTCTCTCCGAGCCCGGTCCCCGACGGCC590.10178204840685218No Hit
AACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC590.10178204840685218No Hit
ATGCAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10178204840685218No Hit
TATGCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10178204840685218No Hit
TTTAATGGGACTGCCCTGTGCCCCACTCGACTGTCTCGCCTCTCCA590.10178204840685218No Hit
GTCAATGGGATAGCTCATTAGGAAGAGAAACAGTGTTGTCAGGATC590.10178204840685218No Hit
TCAAGTGGGAATGAAACAGTTTTGTTCAGCCCATGACTTTGTGCAT590.10178204840685218No Hit
GAGTATGGGATAACTTATCTATTTAATTTATTAAACCTAATGGCCC590.10178204840685218No Hit
CTCAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10178204840685218No Hit
GTCTGCAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10178204840685218No Hit
TATGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.10005692894232926No Hit
AAGCCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.10005692894232926No Hit
CTTATGGGATTATTGATGATAAAGGTATCCTTCGACAGATAACAAT580.10005692894232926No Hit
CTATCCGGGGGGACCGGGCCTTAGTGGACCACGAGAATGTCATCAG580.10005692894232926No Hit
ACGATTGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG580.10005692894232926No Hit
TTTCGGGGATTTACACTACATTATAGTTCAAGTTTACATCATAGTT580.10005692894232926No Hit
ACTACTGGGGAAGGAACCTACCAAGGCCACCACACTCCTATTGTAC580.10005692894232926No Hit
CTATGCGGGGACCGCCTGAATGAGCGAGTGGCCTACCACAGGCTAG580.10005692894232926No Hit
TTTACCGGGAGGTTTTGGTTGGTCACCCCAACGATCAGCTGTAGTT580.10005692894232926No Hit
CACCGAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA580.10005692894232926No Hit
CCTTCAGGGGACATCAAGCTCTTTGGGAAATGGAGCACTGATGACG580.10005692894232926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)