FastQCFastQC Report
Sat 31 Dec 2022
SRR5843727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54705
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCAAGGGATCTCAAAACACCTTTCCAAAAGAAGTTGTTGAAAAT730.13344301252170734No Hit
CCTCCCGGGAAAGTACTTTCAGAATTCAAGGACTAACTTTACTTTT730.13344301252170734No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA710.12978703957590715No Hit
GCATCAGGGCTTCCGATCTAGCCAAGGGGTTTGAATATATTGGTGA700.12795905310300704No Hit
CCTATTGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTTG690.12613106663010692No Hit
AATCCAGGGATACATTTGTGGGGTGTGTTTGTTTGTTTGTTTTTTA680.12430308015720683No Hit
TTACTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC670.12247509368430674No Hit
GTAACGGGGGAGAAGGTGAAGATGGCTGCCGCCCGGGCTGCGGCCC670.12247509368430674No Hit
CTGTTAGGGCGATCTCAGTTGGGGGGGGCAGATGTCAGGCGGTGGC670.12247509368430674No Hit
ACTACAGGGGAGCCCTTGGCAGTGCTGAGTCAGCTCCCCTCACCCA670.12247509368430674No Hit
CTGCTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA670.12247509368430674No Hit
TTCTTGGGACGTGTGCAGCATGTGCTTTCCCCACTGGCCCATCTCT670.12247509368430674No Hit
TGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA670.12247509368430674No Hit
TAGCTTGGGAGGGGCATGGAATAGAGGTATTGTTCTGGGGCCACAA660.12064710721140665No Hit
CCAAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC660.12064710721140665No Hit
CGCTTAGGGGGAGCGGGTGTGTCATTGTTTGGGAAAATGGCTAGGA660.12064710721140665No Hit
TTTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTC660.12064710721140665No Hit
CTTAACGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC650.11881912073850653No Hit
GATACTGGGAGTTCTCATTCACTAGATGTTCAAAGCCACCCTCAGA650.11881912073850653No Hit
ACAACAGGGGTCTCCTGTGCCCATTGTATGCAGTGGGACACTCTGC650.11881912073850653No Hit
ATTACTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC640.11699113426560644No Hit
GGCTGCGGGGAACAGAGATGCAGAAAACACAGAGGAACAGATGTGA640.11699113426560644No Hit
AGCACAGGGATTCAGATAATTTTACTCTGCATCACAATGTATTTCC640.11699113426560644No Hit
ACACTTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCC640.11699113426560644No Hit
TTCGCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA630.11516314779270634No Hit
ATAACTGGGATGAGCTGGCTAGAGGAAAATGTCCATGAGGTTCTGC630.11516314779270634No Hit
AGTGAACGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTT630.11516314779270634No Hit
CATAACGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTG630.11516314779270634No Hit
AAGTATGGGATTTAGACCAATAAGAAAATCCTCCAAAAGATCCCCT630.11516314779270634No Hit
TGATGGGAGTGAAGGAACGGAATCGCAACAAACTGACAAGAGAGAG630.11516314779270634No Hit
ACTACTGGGGAAGGAACCTACCAAGGCCACCACACTCCTATTGTAC620.11333516131980623No Hit
ATCCTAGGGGAAGGGAACTAAGGGGACACCACAAGGGCACCCTCCC620.11333516131980623No Hit
AATACTGGGGTTCCAACTCTTCCCAGAAACCCCACTTGGTAGTTTA620.11333516131980623No Hit
AAATAAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC620.11333516131980623No Hit
CTGGGGAGGTCTCCTCACACCGGTCCAGTGGCCACATTTGCAGCGT610.11150717484690613No Hit
ACCTATGGGAATGTAACTCCAGCAGCAAGCTTTGCTTGTATTTAGT610.11150717484690613No Hit
CACATAGGGATCCTGGAAACTGTCAAAGAAGACAGTAAGGAAAAGA610.11150717484690613No Hit
ACAAAAGGGGCTCTTGGTTGATGGACAAGATAGAGGCCAGGGAGTG610.11150717484690613No Hit
CTCCAGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG600.10967918837400603No Hit
ATTAAAGGGCTATAATTCAATTACACTATTAACCCTTGGCCTACTC600.10967918837400603No Hit
CACCGAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA600.10967918837400603No Hit
AACTCCGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACATG590.10785120190110592No Hit
GTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10785120190110592No Hit
AAGCCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10785120190110592No Hit
CTGGCAGGGATGGGCTTGCCCGTCATAAAATAACTTGCTGCAGGAA590.10785120190110592No Hit
GCGAGGGATCACAGACTTAAGAGCCAAAAAGCTTTTCATCTCTCTG590.10785120190110592No Hit
GCTTTGGGGACTAAACCCTTTACTATAGCAGTGTGTTCCTGATTAG590.10785120190110592No Hit
GCTATGGGGATCTCAAGGCTGAGGCTGTAGATCCATGGTAATAAAT590.10785120190110592No Hit
TTCATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10785120190110592No Hit
AATTCTGGGCCGGTGTCAAAGTATTTAGCTGACTTGCAACCCTACA590.10785120190110592No Hit
CTGCCAGGGAGGACTGTGAGTGAGTGTGCATGTGTGGGAATTCATC590.10785120190110592No Hit
GCCACAGGGATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTT580.10602321542820584No Hit
AACTACGGGGTCCTCTAAGCGTCACCACGACTTCAACGTCCTGAGT580.10602321542820584No Hit
ATGCAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.10602321542820584No Hit
CAATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.10602321542820584No Hit
GCAATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.10602321542820584No Hit
GGCTGCGGGTGAATCTTATAGGCACTTTCAATGTGATCCGCCTGGT580.10602321542820584No Hit
TATGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA570.10419522895530574No Hit
TTAACCGGGAGCACCGGCAGATGGGCAAGGGTGCCAAACACCTCAG570.10419522895530574No Hit
ACAAAGGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCA570.10419522895530574No Hit
ACGTATGGGATGTATATTTTAGCTTGTCATGAAATGTCAACGAAGG570.10419522895530574No Hit
TCTGCTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG570.10419522895530574No Hit
ACTTGCGGGATACAAAAATAAAATATAAACCATAAAATGTACTACC570.10419522895530574No Hit
TGACGGGATGGCTGGGATCATCGCCATCTACGGCCTGGTGGTGGCA570.10419522895530574No Hit
ATTTCAGGGGAGTTGACCAGCCAACCGACTCTGCATTCATCTAGCC570.10419522895530574No Hit
ATCCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAA570.10419522895530574No Hit
CGGGATGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTC570.10419522895530574No Hit
CCAGGGGGCCTGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGAC560.10236724248240564No Hit
ACGCCTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC560.10236724248240564No Hit
TTTACCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.10236724248240564No Hit
TCATGGGGTCAGTGGTGGAGGCCAACTATGACGAGTACGCTCTGCT560.10236724248240564No Hit
TGATTGGGGAGCCTGCGGAACGTCTCCTGCCAGGCACAGATGAGCA560.10236724248240564No Hit
ACTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT560.10236724248240564No Hit
TCCACGGGTTCACAAGGAGCACGGCTCTGCAGGCGTTGAATATTTA560.10236724248240564No Hit
CCGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA550.10053925600950553No Hit
TTTAATGGGACTGCCCTGTGCCCCACTCGACTGTCTCGCCTCTCCA550.10053925600950553No Hit
TTCCCAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC550.10053925600950553No Hit
CCACCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA550.10053925600950553No Hit
TCTACGGGGGGAAGCCAGAGCTGCAGGCTCCTCCCACCTCTCTCCA550.10053925600950553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)