FastQCFastQC Report
Sat 31 Dec 2022
SRR5843700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67263
Sequences flagged as poor quality0
Sequence length46
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAAGGGATTCAAACAAGGAGATTTTCCTCCGCGAGTTGATCTC910.13528983244874596No Hit
TTATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA820.12190951934941946No Hit
CAATACCCCACGGAAAGAGGGAGGCTTGGGCCCCCTGAATATCCCT790.11744941498297726No Hit
GCCAAAGGAAATTCCCTTGACCCAGACCTCGGACTTCCCACCCACA790.11744941498297726No Hit
GCTTGAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC790.11744941498297726No Hit
AACGCAGGGGGGCTGTGACAGCTGATCATTGAAAATATAGCGTTTT780.11596271352749654No Hit
CTAAACGGGGACCCATCTGGCCCACAGTGCCAATGACCACATTATA760.1129893106165351No Hit
GGATCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA760.1129893106165351No Hit
TTCAGCGGGGTAGTCGTCTTGGCTTACCTTGTTGGGTATAAACCTA730.10852920625009292No Hit
CTATAAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA730.10852920625009292No Hit
ATAAGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA720.10704250479461219No Hit
GCACCTAGTTGCCTCATTCCCTAGGACCCCATGAGCATCTTCACCA710.10555580333913148No Hit
TCTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAA710.10555580333913148No Hit
CGCTTTGGGGACCAGCTCACAGGAGTTTCTGGTTCCCAATGATCTG700.10406910188365075No Hit
CTCTGGGGGACTGGGGTGATAATGGCTTCTTTAAAATCCTCAGAGG700.10406910188365075No Hit
CCGCCAGGGACTACCTGGGAGGTCTGCTGTGGCTGTAGCTTTGACC700.10406910188365075No Hit
ACTACCGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC690.10258240042817003No Hit
ACATAGGGGGGGCCCTAGCTGTGTGCCTCTACACCCACTGGGCCTT690.10258240042817003No Hit
ACCGCTGGGGGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTAT690.10258240042817003No Hit
CTGATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA690.10258240042817003No Hit
CATCATGGGACTTTTGACATCGATGCCAATGGCATCCTCAATGTTT690.10258240042817003No Hit
CTCATCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC690.10258240042817003No Hit
CGACATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA680.1010956989726893No Hit
CGCTCCGGGGATTTTCTCCATTATAAGGCCAGTGGATGTAATTTGT680.1010956989726893No Hit
ACACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC680.1010956989726893No Hit
TTTGTTGGGGGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCCTT680.1010956989726893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)