FastQCFastQC Report
Sat 31 Dec 2022
SRR5843647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50014
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA890.17795017395129364No Hit
TACTGCGGGAAGGCCCTGAGTCCTCAGCTCCACAAAACAAAAAAGA690.13796137081617146No Hit
GCGGATGAGAGACTGGCTCAAAAACATCCTCATGCAGTTTTATGAA670.13396249050265924No Hit
AGCTTTGGGACTGCCACAGGAAAACAGTGGTAGCTTAGAGAGGGCT670.13396249050265924No Hit
TGCACAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCC660.13196305034590314No Hit
ATGCGGGGTTGGTGGTGGTAGGTGTTTTTGATTTTTGTTTTTTCTG640.12796417003239094No Hit
ATATTAGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT640.12796417003239094No Hit
TCCGTCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA620.12396528971887871No Hit
CTTTATCTGGGGAGTGCAGACATGGCGGCCCAGAAGGACCAGCAGA620.12396528971887871No Hit
GCCTAACCGGGGAAAGGAAAACAGTCATTACATCCCATAGAGATTT610.12196584956212261No Hit
TTGCAAGGGGTTCTGATTTTCAAATGAGCTAGATGCTCCTCTTTCC610.12196584956212261No Hit
CGTCGCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT600.1199664094053665No Hit
CCTTCTGGGGGAAATAGTCATTTCACAGCAACTTCACTGAGATATA600.1199664094053665No Hit
CACTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC600.1199664094053665No Hit
GCACAGGGGGAAAACCCAGGTCACTGCGACTTTGTCAAGTTGAGGA600.1199664094053665No Hit
TACTTTGGGGGCAGGGCGGTTGGCGAGGAGATGGATCCCGACCTTT600.1199664094053665No Hit
AATTATGGGATCCCAATTGGAACTTGCCCTCCATACTCTCCTCCCA590.1179669692486104No Hit
ATTTATGGGGGTTGTCTCCAGTTGTGTCTCCCTTCCTGAAGATGAT590.1179669692486104No Hit
CACAAGGGGATAGGCATGGAGTTTGGAGGACAGCTTTCTGGAGTTG580.11596752909185427No Hit
AAGCCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA570.11396808893509816No Hit
TCATTAGGGGTCCAGAATGTGTTTGTGGATACCCATGATCTTCGCT570.11396808893509816No Hit
TAAACTGGGAAGGACGTCTCCTGGGACGGGCTGACTGGCTGGAGAC570.11396808893509816No Hit
GATTCCGGGGTCCGTCGTGGATCTGACGTGCCGCCTGGAGAAACCT570.11396808893509816No Hit
ACGCATGGGGACCAGAGAGGTCGTCGACATGTCGAAGTACAAAGCC570.11396808893509816No Hit
CCATACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA570.11396808893509816No Hit
CGATAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG560.11196864877834206No Hit
GTGGTTGGGACGTCCAGCATGTTGTTTGCCTGTGCTCTCCTTGCCC560.11196864877834206No Hit
CTACTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.11196864877834206No Hit
AATAACGGGATCCAAGACACAGGTTCAAGGCCCCAACCCCTGACAC560.11196864877834206No Hit
GGAACGGAGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.11196864877834206No Hit
CCTCAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.11196864877834206No Hit
TAGCCAGGGGACTTGATTTTTTTCCCCTCAGTGAGTGGATTTCGGA560.11196864877834206No Hit
CCGTTTGGGATGCTAAGCTCATCTTCCCTTCTAGTTTCTTTTCCCG560.11196864877834206No Hit
TCGCCCGGGTTTGTAAAATCCCGTGTAAGAGTGAGTGTTTAGCGTG560.11196864877834206No Hit
CTGACAGGGGCGTGGTGAATAAGGAAGCCGGGAGCGGCCCTGCCTC550.10996920862158595No Hit
GCGTTTGGGTCGGCATGGGGATGACCGAGGTCTCTATAAACAAGGA550.10996920862158595No Hit
TCAATTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA550.10996920862158595No Hit
TCTGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTC550.10996920862158595No Hit
TTCAATGGGGTGTCATGATCGTGAGTTGTCGCAGTGTCTGTACCAA550.10996920862158595No Hit
CGAACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGC550.10996920862158595No Hit
CTAGGTGGGGATGTTAGAGTAGGGGGGTTTGTTCTGTAGTCAAGAA550.10996920862158595No Hit
GGGTCAGGGAGAGTGAACCGCGGAGAAGGTGAAGATGGCTGCCGCC540.10796976846482985No Hit
CTCGGTGGGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG540.10796976846482985No Hit
ATCCGGGGAGCATCTTCACCATGAGGGCCAAGCCCAGGTTGCTTCT540.10796976846482985No Hit
GAGTAGGGATGACAAGGACGGGCGGCTGCTCCAGGAGTTTAACAAG540.10796976846482985No Hit
CTTGCCTCGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTA540.10796976846482985No Hit
GCTTCAGGGGCCCGCAGACGTGGCATGAGAATGGCTTCTCGTTGGT530.10597032830807374No Hit
TTGCCAGGGATGTGAAGAAATAAGTATTTAGTTTTCTGTAGGGTTT530.10597032830807374No Hit
GTTAATGGGGGTGGAAGGACGTGGTCCTACACACCTTCCGCCTAGC530.10597032830807374No Hit
TTTACCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA530.10597032830807374No Hit
CCAATTGGGGGGTACACTAACACTCACCTGCTCCCTTTCGTTCCGC530.10597032830807374No Hit
GTTGCTGGGGAGGACCCAGATTGAAGCAAGATGGCGAGCGCGCGGA530.10597032830807374No Hit
TCATTTGGGCTCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGC530.10597032830807374No Hit
TACTTTGGGAATGATGAGTCTGCAGAGTGCTTCGCTGAGATGCTGA530.10597032830807374No Hit
TAACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATT530.10597032830807374No Hit
AGGCCTGGGGATCTCGCTCAGGGGAGTAAGGAAGATATTGAGCGCA530.10597032830807374No Hit
CGTGACGGGAGAGCCTGGGGCTGCTCGACCAGAACCCTCACCTGGC530.10597032830807374No Hit
TTATCAGGGGTTTTTCCTTTAGCCTTTCTCAGCACAAGCCCTCTGT530.10597032830807374No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA530.10597032830807374No Hit
GTACGCGGGATGATTAGAGCTTCTAATGACCACACAGAAGGCAAAC530.10597032830807374No Hit
ATGTATGGGTCGCGACGGGGGGCCAGAGTGTGGCCCAATCACAGCT520.10397088815131764No Hit
CACGCTGGGGGGCTAACTTCCCGCTCGGCCCCAGCCCGGAGGGCCC520.10397088815131764No Hit
ACTCATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC520.10397088815131764No Hit
GTTCTTGGGGTCCTACTAAACGGAAGGGGCCGGGAGAGGCCGCGTT520.10397088815131764No Hit
ATTAGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA520.10397088815131764No Hit
TAACGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA520.10397088815131764No Hit
CGCTATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG520.10397088815131764No Hit
TACTACGGGGCCAAGCAAGACCAGGTGGTACTGATAGATACAACAG520.10397088815131764No Hit
TCGCTTGGGGAGAGGGATGTATGTGCAGAGGTCAGAGGACAGCCTT520.10397088815131764No Hit
GGAACAGGGTCGAACCTCCAGGCGCTCATAGATAGCACCCGGGATC520.10397088815131764No Hit
AAGTGAGGGGATATGGTGACTGTGCCCTTCAAATTATGGATGAAAA510.10197144799456152No Hit
ATGTTCGGGGTGGGAAAACAAGCATATCACATGGTTTGCAACCCTT510.10197144799456152No Hit
GTGTAAGGGGTTGACAAGCCCATCCGTTTGTCTGGAAGATAAAGGA510.10197144799456152No Hit
TCATTAGGGGTACATATAAAGTTCCAGGCCAGAGCAGCCGATAGTT510.10197144799456152No Hit
TTGCTAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC510.10197144799456152No Hit
TTGCGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA510.10197144799456152No Hit
TTTGCTGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATAC510.10197144799456152No Hit
TCTAATGGGCACCAGAAGGGCTCGTTAGGGATGTAAGAACACAACA510.10197144799456152No Hit
AAACCCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC510.10197144799456152No Hit
TGCAGTGGGGTCTTTGCTAAGGGTCACATTGAAGTAACGCTACTCC510.10197144799456152No Hit
ATGACCGGGACCGTATCTCCCTGCAATTTGACTTCTTCGAGACTGA510.10197144799456152No Hit
TCTGCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA510.10197144799456152No Hit
TGAACTGAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA510.10197144799456152No Hit
TCAAACGGGATGTATGATTGATTGCTTTTTAAGTGACTATTTTTCC510.10197144799456152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)