FastQCFastQC Report
Sat 31 Dec 2022
SRR5843643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64621
Sequences flagged as poor quality0
Sequence length46
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACATGGGGGAAAATGGCCAAGTGAAACAGTTGTTGGTCATTGAA820.126893734235001No Hit
CGCATAGGGGGAAGGTAAATTGGTCTGTGGCACAGTGACATAAACC800.12379876510731805No Hit
ACAGTTGGGGTCTGGAAGAGAAAGAGACAGAACAAAGATGGAAGTG770.11915631141579362No Hit
CTCTCAGGGGACTGTCGCTAAGTTCATGGGCCTCACCTGTCAGGCC770.11915631141579362No Hit
ATAACCGGGAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCG760.11760882685195216No Hit
TCACACGGGGCAAGACGTGTAGGGTGGACAGTCCGCAGGGGTGTTC750.11606134228811067No Hit
GCAAGTGGGCACACTAACCTCGGTGGAAAAATAACCATTCCCTGCC750.11606134228811067No Hit
GTACATGGGATTGTTGGGGGAGGGAGTCCAGAAATCTTGAGAGTGC740.1145138577242692No Hit
GCGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT730.11296637316042774No Hit
CTTACCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA710.10987140403274477No Hit
CTTTGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA710.10987140403274477No Hit
GACTATGGGGTCTGACATTGGCGTCTTTGCTAAGGGTCACATTGAA710.10987140403274477No Hit
CAAACTGGGGCTCTGTGAGTGTGTGCTGTCTGCTAAGAGAACATCC710.10987140403274477No Hit
CTGCAGGGGGGACTTTGGCGTGGCGTCACACCCGGAAGCAGCCCTG700.10832391946890331No Hit
CTTATCGGGGAAGCACGGAATCAGAAGAAAGAAGCCAAGGAATTAG700.10832391946890331No Hit
TTTAGTGGGGACGACGCGTGGGGTCAAGGGTCACCGGCAAGATGGC700.10832391946890331No Hit
TAATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC700.10832391946890331No Hit
CGCATTGGGGGAACGCGGCTAAGTGTTGGAGATTCGGAGCTGCAGG700.10832391946890331No Hit
CCGCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA690.10677643490506182No Hit
CAACCTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA690.10677643490506182No Hit
CATCATGGGTAAGCTCTTTTGGCCTGGGTACAGCAGAAAACTAGCA680.10522895034122035No Hit
AATGTTGGGGGGCTGAGCCGCGGCAGGAGAGTACTGAGAAGCGTCG680.10522895034122035No Hit
AGTTGGGAGAGAAGCCTTACCTGTGTGATAAGTGTGGGCGTGGCTT680.10522895034122035No Hit
ACACTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA680.10522895034122035No Hit
AGTCGGCGGGAGGGAAAGACGACTAAAAGACAGGTCTCCTCACACC680.10522895034122035No Hit
AATCGGGGGGAGCAGACTGCTGATTGGTGACTCTGGTCTGCCTAGC680.10522895034122035No Hit
ACGCGGGCATCCAGAAGGAGTCAACCCTGCACCTGGTCCTCCGTCT670.10368146577737886No Hit
CCGCACGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGAT670.10368146577737886No Hit
TTAGTTGGGGAAGTAATTTTGAACGTACGCGATTTGTAGGTTTTGT670.10368146577737886No Hit
AGCTTAGGGGAGGGCTGTGCGCCCTTGGCGTGATGGAGCAGCAGCA670.10368146577737886No Hit
ACGACAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA660.1021339812135374No Hit
CTTTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA660.1021339812135374No Hit
ATCTAGGGAGAGAAAGGCGGCAGCTGAGCTGCTCCAGTCTCAGGGC660.1021339812135374No Hit
GATCCAGGGGATGTGCCCTGAATTCTTAGATTATCAACTCCCTGCT660.1021339812135374No Hit
CCTGGAACTAGACTTACAGGTATTTGGGAGCCACTATATAGTAGCT660.1021339812135374No Hit
CTGTTTGGGGAAAAAAACCTGAAATTACTCATTGATGAAAAACAAA650.10058649664969593No Hit
TAGTGCGGGGGCTTTTGAGCGGCGGCGGCCCGAGCTAGTGCGTCCG650.10058649664969593No Hit
TAGATAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC650.10058649664969593No Hit
CCTACAGGGACATCCTGTCGGTTATATTTATCTTAGCATGAGATTT650.10058649664969593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)