FastQCFastQC Report
Thu 19 Jan 2023
SRR5843621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251890
Sequences flagged as poor quality0
Sequence length46
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT200.001253902840.022
CTTCGCA200.001253902840.029
TAATACG200.001253902840.038
TAATAAG200.001253902840.01
AACCGCT200.001253902840.021
AATCCGG257.777309E-540.02
AATCCCG200.001253902840.01
CCTTAAC257.777309E-540.038
GGTACAA200.001253902840.09
TCCGCTA257.777309E-540.032
CGTATCG304.852369E-640.01
GATAACG200.001253902840.010
ATAGGGT850.040.04
CGTGCTC200.001253902840.022
CGACGGA200.001253902840.033
CGCGGAC200.001253902840.019
CCCTATA200.001253902840.030
TCCGATG304.852369E-640.026
TAGACGG200.001253902840.026
AACCCAC200.001253902840.039
Produced by FastQC (version 0.11.5)