FastQCFastQC Report
Sat 31 Dec 2022
SRR5843540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73603
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA1100.14945043000964636No Hit
CCATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA1090.14809178973683138No Hit
GGCACTGGGGGTTTCTTTTAGTGACAAACTGGTTATTCAGAGCCTG1060.14401586891838647No Hit
CTTTGTCAGGGGAGGGCCAAGCCCAGGTTGCTTCTATTTGCTTCAC1050.1426572286455715No Hit
CGTCTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA1050.1426572286455715No Hit
AATTTAGGGGTGGCCAGTAAGTGGTGTGGAGATTACGAAGGGACAT990.13450538700868173No Hit
TCAGCAGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG970.13178810646305178No Hit
GCCCATTGGTACACACTAACCTCGGTGGAAAAATAACCATTCCCTG970.13178810646305178No Hit
CGCAAAGGGTCCGGCAAGATGGCGGTGGCGCTGACCTTCCGCCGAC950.12907082591742186No Hit
GTCAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA950.12907082591742186No Hit
CCTTGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC940.12771218564460687No Hit
TGACCTGGGGGCCCCAGAGCGGAAGAGGGAAGTGAGTCGGACCGGG930.12635354537179191No Hit
GCGTTTGGGGACAGCACTCATGGAGAGACTACCTCTGCTGAAGACT930.12635354537179191No Hit
GTTGTAGGGGCTGCTTGTCTCGGGCGAGATGGCTTCAAGGTTACTT930.12635354537179191No Hit
CTGCATGGGAGATAGGATTGTGGGCCATCGGAAGGAGCTCTTTCTC920.12499490509897694No Hit
GCAGCAGGGCGATCTGTCTGCGGGGGGAATTCTGGACATTAATTAG920.12499490509897694No Hit
CAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT920.12499490509897694No Hit
CTGACAGAGGGAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGC900.12227762455334701No Hit
CCAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA900.12227762455334701No Hit
GTTTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA900.12227762455334701No Hit
CTCTCTGGGGGCTGGGATAAGCGCTACTGCCTGGACAGCCTGGATG900.12227762455334701No Hit
CACGCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC880.11956034400771709No Hit
GATTGTGGGGGAGCCTCTCCTCTGGCCGCCGCGCGGGAGAGAGGCC880.11956034400771709No Hit
AATCTTGGGCCTGTTCTCACATCCCCAAGCTTAGATAGGTGTGGAA860.11684306346208716No Hit
CAATTAGGGGGTACACAGTGTGTGCTTGGATGGGAGGAAAAGCTCC860.11684306346208716No Hit
GGCATGGGGGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGT850.11548442318927217No Hit
CTAGACGGGGGGTGGCTTTCGATTTTTGGCGTTCAGTAGTCGCGGT850.11548442318927217No Hit
CTCTTAGGGATGGCCCAAAAACTATAGTATAAGTTTGAAATTTCGG850.11548442318927217No Hit
GATTACGGGGACACCGGTCCAGTGGCCACATTTGCAGCGTGCCCTT840.11412578291645722No Hit
TATTAGGGGTTGCCCACTGCTTCTGGCTGTGGGCCACATGAGGGTA840.11412578291645722No Hit
GCTGATGGGTCCATCTGAGCCAGGGCTCTCTCCTGAGATGTCTGTG840.11412578291645722No Hit
ATGCAAGGGGCCTTTTTACTACCTTCTCTCTCAATACACAACACCT840.11412578291645722No Hit
TGCATCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA840.11412578291645722No Hit
TCTCTGAGGGCATGGGCTACAGAATGAGTTCAAATGTTAGCCTGGG840.11412578291645722No Hit
ATGACAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA830.11276714264364224No Hit
CACTCTGGGGGAAGGAGGAAGTGTTTTGCTGCGGGGTCCTGGCCTG830.11276714264364224No Hit
TTCGCAGGGGGGAATTCGAGCTCTTGATGATGATACTTAACCAAAC830.11276714264364224No Hit
GTTAGAGGGGGGAACTGAGCGAGCCGGTGTTGGAGTTCCAGGCGCG830.11276714264364224No Hit
ACTACCGGGGCCTTGCGGCATCCCAGGATGGTGGCGCCCAGCGGTG830.11276714264364224No Hit
CAAGAGGGGAGTACAAGCTGTCATGGCGGCCCGACTGTTAAGCTTG820.11140850237082726No Hit
TCAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA820.11140850237082726No Hit
GCATCTGGGGAGCGGCGGCGGCGGCAGGTGAAGGCGCCCAAGTCCG810.1100498620980123No Hit
ACTCCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA810.1100498620980123No Hit
TTTCTAGGGTCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCAC810.1100498620980123No Hit
TCTGTAGGGGGGAGGAGGCTTAGGGACGTTTCCTCGGGAGCCATCC810.1100498620980123No Hit
AGCTTTGGGGGCACTCGGGTGGCGGCCAGAAACCGGCAGGACTGGA810.1100498620980123No Hit
CGCCAAGGGAGAAAAAGGCCACGATGCGACCCCAGTTTACTCCATC800.10869122182519733No Hit
CGCGGGGGATCTAACACCGGGGGGAATTCTGGACATTAATTAGGGC800.10869122182519733No Hit
ACGCAGGGGCCCATTGGTACACACTAACCTCGGTGGAAAAATAACC800.10869122182519733No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGC790.10733258155238237No Hit
CAATCCGGGACATCAAGAAGTTTGCATCAGGTGGCTGTGACAACCT790.10733258155238237No Hit
GCCTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA790.10733258155238237No Hit
CATTGCGGGACCGGCAAGATGGCGGTGGCGCTGACCTTCCGCCGAC790.10733258155238237No Hit
GCTCTTGGGCACCCAGCTGGATTGACACCGGACTCTCAGAGATGCG790.10733258155238237No Hit
TCCGATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT790.10733258155238237No Hit
TCTTGCGGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGC780.1059739412795674No Hit
ACGAAAGGGGGCTTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTG780.1059739412795674No Hit
TGCGGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA780.1059739412795674No Hit
TCTGGTGGGGATAAGCCCAGACTGCCCAGCTGAAAAATCCACGAAA780.1059739412795674No Hit
ATCGCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA780.1059739412795674No Hit
AACTTAGGGATAGACAACATTACCCATTTTAGAGGTGAAAAAAAAA770.10461530100675245No Hit
AGATAAGGGGAAGGCAAGGAAGGCTATGTCCAAACTGGGTCTTCGA770.10461530100675245No Hit
TGCGCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA770.10461530100675245No Hit
CAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAGAGCTAA770.10461530100675245No Hit
CTCCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC770.10461530100675245No Hit
CACCTGGGGGAGTTCCATGATGGCGTCGGCGGTGCTGTCAGCGTCT770.10461530100675245No Hit
TGGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC770.10461530100675245No Hit
AGTTACGGGGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGA770.10461530100675245No Hit
TTTAGCGGGCGATCTGCTCATGGGGAACAGCCAAATGGATTGACTA770.10461530100675245No Hit
GAAACAGGGAGGTGGCTTAAGTCTCCTGCCATCACTCTGTCCCTGG770.10461530100675245No Hit
ACCACTGGGATCCCAATGAAGACTTTGAATATGAAGACTGTATATG770.10461530100675245No Hit
ACGTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA770.10461530100675245No Hit
GTCTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC770.10461530100675245No Hit
ATGCATGGGGGCAGTTCCATGTTGAATTAGAGTTTCGCAAGTAAAG770.10461530100675245No Hit
CAATTAGGGAAGTCAGAGTAGGGTGGAAAGCCATTGGGAAAATATT760.10325666073393747No Hit
TTTGCCGGGCACCATCAAGACCAAGCGTACCATCCAGTTTGTGGAC760.10325666073393747No Hit
CAAACGGGGAGTGTGCAGATCCCTTTATGTTGCCTCATGTAAGCGG760.10325666073393747No Hit
GGGCCCGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCC760.10325666073393747No Hit
GTAGTTGGGGGACCTCTGGCTGTGTTCAGTGAGAGCCATGATGCTG760.10325666073393747No Hit
GCAACTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC760.10325666073393747No Hit
ACTAGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA760.10325666073393747No Hit
CTACAGGGGGTTATTATTATTTTGTTTTTTTGTTTTTTTTGAAGCA760.10325666073393747No Hit
TCGACCGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCA760.10325666073393747No Hit
CTATGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC750.10189802046112252No Hit
GTATCAGGGGGGCAGGGGGAGAAAGCTGCAATGTCATCCTAGCAGA750.10189802046112252No Hit
GTATAGGCGGGGCGGACACCAGGACTTCAAGATGGCGTCACTCGTG750.10189802046112252No Hit
TGCGCGGGGCAAGGGCCCAGGGATCATCCTGATATTGTTGATTCAT750.10189802046112252No Hit
ATGCAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA750.10189802046112252No Hit
GAACTGGGGATTGGAGCAGTGAAGAACCCGAGGAAGAGCAGGAGGA750.10189802046112252No Hit
TAACTAGGGGGTCTTTAAAAGAAGGAGCTGAAGCCCCTTAGGAGGT750.10189802046112252No Hit
GCAACCGGGAGTCTAACAAGGCACTCACTGTATTGAAAAACAGGCA750.10189802046112252No Hit
ATTCAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGG750.10189802046112252No Hit
GACCGAGGGGGAAGTCACCTGTGGGCCAGTCATGTGTTGACCCTTT740.10053938018830753No Hit
GCTAGCGGGGCGTGGGGTCAAGGGTCACCGGCAAGATGGCGGCGGC740.10053938018830753No Hit
TATGCAGGAGACACAGAGTCGTCACCAGCGTCCTTCGGAGCGGAAG740.10053938018830753No Hit
CACACGGGGTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCC740.10053938018830753No Hit
TCTTAAGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC740.10053938018830753No Hit
TCGCCCTGGTGATAGTTTCTCCACATTTGATACGCCTTACTGCAAA740.10053938018830753No Hit
ACCATGGGGGCTCCCAAGCGCGGCGGCGGCAGCGTAGTTCCTAGCC740.10053938018830753No Hit
CTATACGGGGGTAATGGGCGGGGCCTGTCTGCTAACATTAACCCTG740.10053938018830753No Hit
TGTAACGGGGGTCGCTAGGAAGCAGGCAACCGGAGGGAAGATGGTT740.10053938018830753No Hit
ATTTCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA740.10053938018830753No Hit
ATTACCGGGGCGCAGCGGCTGGGCAAGCGCGTGCTGAGCAAGCTGC740.10053938018830753No Hit
TGGGCTTGCTGCTGGGACTGGTCAGGCTGATCCTGGACTTTGTCTA740.10053938018830753No Hit

[OK]Adapter Content

Adapter graph