FastQCFastQC Report
Sat 31 Dec 2022
SRR5843532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85062
Sequences flagged as poor quality0
Sequence length46
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCGGGTCCAGTGGATTTTTTTAACTATTATTCAAAAGCAAAA1230.14460040911335262No Hit
GTCACAGGGGGGGCAGAGTGGGGACTCACAGAGGCCCCCAGCCCTG1180.1387223437022407No Hit
GTCTTAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCT1090.1281418259622393No Hit
AGCTTGGGGACTCAGAACAATTTAACTTGCTAATTATATCACCTTC1070.12579059979779456No Hit
ATCTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA1050.1234393736333498No Hit
TCTTAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC1050.1234393736333498No Hit
CTTTTAGGGGCCTTTGAGAGGTAGGGACCCAGACCCTATTCAGATC1010.11873692130446027No Hit
TTCAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA980.11521008205779314No Hit
ATACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA980.11521008205779314No Hit
GGACCTGGGAGTTGAAAGAATAGAATGCTGCTGAAGGTTCCTTTAG980.11521008205779314No Hit
TCAATTGGGGAGTGGAGGGCCCTGCCATCCGAGTGCTCTAGCCGCC970.11403446897557076No Hit
CTATACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA960.11285885589334838No Hit
TTGTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA960.11285885589334838No Hit
CAGAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA950.11168324281112602No Hit
TCTATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA940.11050762972890363No Hit
TTGACAGGGAGAGATGGAGCAGGTGCGAAAGCAAGCCCACTGTTTC940.11050762972890363No Hit
TTCTCAGGGATCAATTTAGTGACTTGACTCCAGAAGAATTCAAGAC940.11050762972890363No Hit
ATTCTTGGGAGTATTTATTTATTTATTCATCGTAACAGCCTGTGCA940.11050762972890363No Hit
GCTGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC930.10933201664668124No Hit
TTATTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA930.10933201664668124No Hit
GACATAGGGACAGTAAAGTCAGATGAACCAGAGGAGAGGCAGATGT930.10933201664668124No Hit
CTAAAAGGGGAGACCCAGGGCTAAAGCTGAGAGATTGAAAAATGCA930.10933201664668124No Hit
TTACTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA920.10815640356445885No Hit
TTGCAGGGGCACATCCACGAGAAGAGCACAAACCAAGACCATAGCA910.10698079048223649No Hit
GGTGACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA910.10698079048223649No Hit
TATTCAGGGATGGTAAATGACTTCTAAGTCCTGTTAAAGTTGACAG910.10698079048223649No Hit
AGACCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA900.1058051774000141No Hit
CCAGTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT900.1058051774000141No Hit
CACTCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCT900.1058051774000141No Hit
TGTTTAGGGACAGCCAAAGGGGACTTGAAGGAGACGGGCGACACGG900.1058051774000141No Hit
CTGTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA890.10462956431779172No Hit
TCAGTCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA890.10462956431779172No Hit
TTTTAAGGGGGCACATCGAGACTGTGGCCATGGAGGGAAACAGTGG890.10462956431779172No Hit
GGCCACGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT890.10462956431779172No Hit
CCAGTTGGGAGGACTCTCAAAGAAGGAAACTAAAGACCCGTCAGTG890.10462956431779172No Hit
GCGCGGCGGGGAATTTAAGCTCCAGCAGACCAACTGCCCCAAGAAG890.10462956431779172No Hit
TCCAACGGGACTTGTTTGCTGTGAGCATTACATTTGGCCCAGGCCC880.10345395123556936No Hit
TACGTCGGGAGAAACATGACTTGATTAGACAAGATAAAAATCTTGG880.10345395123556936No Hit
CGCTCCGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC880.10345395123556936No Hit
CGTATGGGGAGAGTGAACCGCGGAGAAGGTGAAGATGGCTGCCGCC880.10345395123556936No Hit
TTCACCAGGGGACGCTCTTCCGATCTTTCAGCGGGCCTTTTGTGAC880.10345395123556936No Hit
ACTACGGTGGGGGTGGGGATTCCTTGTGTTAAGTGCCACACAACTG880.10345395123556936No Hit
CAACCAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC880.10345395123556936No Hit
GCAAATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC870.10227833815334697No Hit
CATGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC870.10227833815334697No Hit
CTATCTCCGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTA860.10110272507112458No Hit
TAATCTCGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTA860.10110272507112458No Hit
TCTTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC860.10110272507112458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)