FastQCFastQC Report
Sat 31 Dec 2022
SRR5843531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81181
Sequences flagged as poor quality0
Sequence length46
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCGGGTCCAGTGGATTTTTTTAACTATTATTCAAAAGCAAAA1180.14535420849706213No Hit
CATAGAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC1020.1256451632771215No Hit
ATTTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA1010.1244133479508752No Hit
AAAATTGGGCAAACTCATGCATATCACATAGTTAATCCAAGTCCAT970.11948608664589006No Hit
GTGCTCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC960.11825427131964376No Hit
CTTTAAGGGCAAGGAGGCAGGCCCGGCCCCTGCTGACCTGTGCTTT950.11702245599339747No Hit
TTAACGGGGCGCCCCTCGTTTATGAACTAGCCTATCGCAGACGGTA950.11702245599339747No Hit
TCTTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC940.11579064066715118No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA930.11455882534090489No Hit
CTAAAAGGGGAGACCCAGGGCTAAAGCTGAGAGATTGAAAAATGCA930.11455882534090489No Hit
AACAAAGGGGTGGTGGGAGGTGTTTGGGGGCCCTTAAGAGGTTGGC920.11332701001465859No Hit
TGCTATGGGGGTCAACAGGAAGGCCCAGCAACTCCACCCTCCTGAG910.11209519468841232No Hit
TTGTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA910.11209519468841232No Hit
TTCAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA900.11086337936216603No Hit
CTGTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA900.11086337936216603No Hit
GTCTTAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCT900.11086337936216603No Hit
ATACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA890.10963156403591974No Hit
CGTATGGGGAGAGTGAACCGCGGAGAAGGTGAAGATGGCTGCCGCC890.10963156403591974No Hit
CACTTAGGGGCGGCTGGACTCTCAGGGATTCTGAGCCCAGAGTGAG890.10963156403591974No Hit
ATGCTGGGGGGGGCCCTGCAGGCTGTGGGGCCCAGTCCTGCCGCCT890.10963156403591974No Hit
CAACCGGGGTGCCTCCTGGTCATCCCATGTCCTGTTTTTCTACAAT890.10963156403591974No Hit
CCGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA880.10839974870967345No Hit
CAACGAGGGCACCGCAAATGCTTCTAGATGGACTGAGCAGGTGCCA880.10839974870967345No Hit
GCTTCAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC870.10716793338342714No Hit
TCAACAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA870.10716793338342714No Hit
CACGCATGCCACAACCACCCTTTTCTCTCGCTGATCTTCAGTAGTC870.10716793338342714No Hit
TCTTAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC870.10716793338342714No Hit
ACTGCTGGGAGAAACAGATCGGCATAGAAGCGGCTGATTCTGATAG860.10593611805718085No Hit
TTATTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA850.10470430273093459No Hit
TTGACAGGGAGAGATGGAGCAGGTGCGAAAGCAAGCCCACTGTTTC840.1034724874046883No Hit
TATCGCGGGGGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTAT840.1034724874046883No Hit
AGCACTGGGGAACTTGCCACTGTGCCTGCAACCTTGTCTGAGAGGA840.1034724874046883No Hit
CTTTTAGGGGCCTTTGAGAGGTAGGGACCCAGACCCTATTCAGATC840.1034724874046883No Hit
CCTTGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA840.1034724874046883No Hit
TCACGAGGGGGGCAGTACTGCAGGGACAGATATTACAGTCAACAGT830.10224067207844201No Hit
AGATGTGGGTGATCGTTTTTAGGATATTGTGAGTAATATAGATTGG820.1010088567521957No Hit
GCTGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC820.1010088567521957No Hit
TTACACGGGAGTTGTGAACTGGCTTCGCCGTTTACCAGCATGGTGA820.1010088567521957No Hit
ACTACGGTGGGGGTGGGGATTCCTTGTGTTAAGTGCCACACAACTG820.1010088567521957No Hit
TTATATGGGGTCTAGCAGACAACCTGAGCTGCAAGATGTCTCCCAT820.1010088567521957No Hit
GTCACAGGGGGGGCAGAGTGGGGACTCACAGAGGCCCCCAGCCCTG820.1010088567521957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)