FastQCFastQC Report
Sat 31 Dec 2022
SRR5843500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45883
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCAGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAG690.15038249460584532No Hit
TCAATCAGGGGGCAGAGAAAGAGAGCGAGAAAAACGATTCAGCTTC630.13730575594446745No Hit
GGTACAGGGGAATTTAACAGAGTGATCATTCCAGTCAAACGAGGCG630.13730575594446745No Hit
GACGGGATGTTAAGTAGGCATCTCAGCCTTTGTCCCTGGTTTGAAA610.1329468430573415No Hit
AGCTCCGGGAAGGCAGACGATTCGTCTCTTGACAATGCCACCCTGA600.1307673866137785No Hit
TACCCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT590.12858793017021555No Hit
CGAGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA550.11987010439596364No Hit
TTTTATGGGGGCGTGCGTCCTCACTCTGAGTACCCCTCTGTCTACG550.11987010439596364No Hit
CATCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCC550.11987010439596364No Hit
GTATTTGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT540.11769064795240068No Hit
CGCAATGGGATTGTGGATGCAGTTTATGTACCTGTAATTCATAATG530.1155111915088377No Hit
GAACCTGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG530.1155111915088377No Hit
ATCGTTGGGCCAGGAACACGGGCCTATTCGGAGTTGTTCTGTCTTT530.1155111915088377No Hit
CACATAGGGGGTTGGTGAGTGGGCTCCTACCGACCGAGGTTTAGGC530.1155111915088377No Hit
CGCTAGGGAGATTCTTCAGGAGAGAGGCTCTCGAATGTAAGCGGAC520.11333173506527473No Hit
CTGCCAGGGACCCGGCAGTGATGCCCGCCGGCTCACCCTTCAGCTG520.11333173506527473No Hit
CGCTTTGGGATAGAGAAGCTGCATTGTGGATTATATGGGAATCACT520.11333173506527473No Hit
CGCTTTGGGGGGCCGGTGTGTACCCGTGGTGTGTGATTCAAGCCAG520.11333173506527473No Hit
CATCAAGGGAATGTGGAGCAGCAAGAGGCTGAGCCCTCGTCCTTGC520.11333173506527473No Hit
CACCGGGATGATGATGGGGTTGAACACTGAGGATGCCCAAGGCCCC520.11333173506527473No Hit
CAAACAGGGAACTCCCTGCCAACACAAGAGCATTTATGGCTTGAGG520.11333173506527473No Hit
CCAGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA510.11115227862171174No Hit
GCGCTATGGGGAGCTGAGCTGGAACCAGGCCATGGATGAGGGGTGG510.11115227862171174No Hit
TAAATAGGGGTGACAAGAGAATGATGTGGTGTGTATCATACTCCAT510.11115227862171174No Hit
TAACTCGGGAGAGACAGGTGCATTCAGAGCAGCCTGGGCACCACCG510.11115227862171174No Hit
GAATATGGGGTATACAGACTGGTTGGGCGCTACTCCCTGAGTATGG500.10897282217814878No Hit
TATCTTGGGAGTACGAAGTTCTTTCATGTCTAAATCTGAAGCTTCA500.10897282217814878No Hit
GCAAGGCTTTCTATCTATGTATATTCTGCCAGAAATGTTGGACCTC500.10897282217814878No Hit
TGCCCTGGGACAGCGTTCTTTGTCAGTATTGTGGTAGTGCAGTGGG500.10897282217814878No Hit
CTCTTTGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATAC500.10897282217814878No Hit
GCGCTTGGGAAACTAAGGAAGTCAAAAGGTAAGCATTCGATCTGGT500.10897282217814878No Hit
ACATGTGGGGGAAAAACCATGCTGGTACCAATCTAAAAGCTTCGTC490.1067933657345858No Hit
ACACTTGGGAGAGGTACCATCTGCAGCTTATTTTAAAAGCCATCAG490.1067933657345858No Hit
TGCGCAGGGGAGGCCTCAGACCGAGGCTCAGAACATGGGTCAGATG490.1067933657345858No Hit
GTTCTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT490.1067933657345858No Hit
CATAGTGGGGATTGAATACTGCCACAGCAAAGGCATCGTGGTGACA490.1067933657345858No Hit
TGCTTGGGGGGAAAGTGTAAGAGGTCCAGCCATAACTAGAGCATTA480.10461390929102281No Hit
CATACTGGGCCGAGATTTCACCACCAACAAGTGCAAGGGTTTCGGC480.10461390929102281No Hit
TATCCTGGGGTTAGGGTTCCCTTCTGTCTGGCCATGCAAGAGGCTT480.10461390929102281No Hit
CTAGCAGGGACTACTTGTTGGACATTGTAAATGATGCATTTCTTTA480.10461390929102281No Hit
TACCTGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTC480.10461390929102281No Hit
TCTTCAGGGGAAGACCCTGACTGGCAAGACCATCACCCTGGAGGTG480.10461390929102281No Hit
CAATTAGGGAAGGCTGTGGCATAATTGCTTTGCAAAACACAGGCAT480.10461390929102281No Hit
TTCATAGGGTCAAGGCGGAGGGTCAGCGTTGCTACCGAGAGAAGAA470.10243445284745985No Hit
CAAATTGGGGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCCTTT470.10243445284745985No Hit
ACTCGCGGGCGTGGCAGAGGGAAGGAAAGGGGCGAGTGGGCTGGGC470.10243445284745985No Hit
ACGCACGGGATCTACTAAAACAAAATATGATGCATTCACACACACA470.10243445284745985No Hit
CTGGCTGGGGTTAACAGTGCATGGTCATCTGAAGGGTCAAAGAAAA470.10243445284745985No Hit
TAGCTGGGATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACC460.10025499640389686No Hit
GCTCAAGGGCAGCCACCACGGTCCTCTACTGCAAAAGTAACTATCA460.10025499640389686No Hit
TGGATGGGGAGGTTGATCCAGTGCTCAGTACCAGTACCTCCATGTG460.10025499640389686No Hit
ACCTACGGGATCTTGGAGCACTCTGTCACGGATGGATTGCTGGGAC460.10025499640389686No Hit
ATGAAAGGGATGGACATCAGACAGTGCAGGAGAAGAATGTGATGCA460.10025499640389686No Hit
CTGCTTGGGGTTAGATGGGCTGGGTCGCATTCGGGACCAGCTTTCT460.10025499640389686No Hit
CTCCTCGGGCCAGGTGAGGGATGTCTGACCTGAAAGAAGAAAGTAT460.10025499640389686No Hit
ACCACTGGGAGAAACCAAGTCCTGGGTTTGTAGTGCCCACTCTGGC460.10025499640389686No Hit
TATTAGGGGGAGGTGGGGAAGATGGCGGACAGCAGCGGTCGTGTGG460.10025499640389686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)