FastQCFastQC Report
Sat 31 Dec 2022
SRR5843443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38930
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCCGGGAAGGGAGGGAAGGTCCACAGCCTGTCAGGCTGACAGG530.13614179296172618No Hit
GCCACCGGGTACGCTGACGGCATTCAGAGTACGCTGATCGGCTTAT500.12843565373747753No Hit
GTCTCTGGGAGGGGGGAGTGGCCAGGCGGCTGGTTGTGGGCCAAGT480.12329822758797841No Hit
ATTTATGGGACCCATCTGCCCTCCTAATATTGAAAGTATGGAGATC470.12072951451322887No Hit
TCTCTGGGATTAGTGTGGGTGTCTCTGTTACTCCACTGCCTGTATT460.11816080143847933No Hit
GCTAGTGGGATTCAAAGCTGGTGAGAACAGAAAGGAGCTGAAGAGG460.11816080143847933No Hit
CACAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCT460.11816080143847933No Hit
CATATAGGGGGACTTTCCGGGTAAAGATGGCAGAGCGCGGTTACAG460.11816080143847933No Hit
ACTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA450.11559208836372976No Hit
TATGTCGGGGGGGGCAGACAACATGGCGGCGTCCGTGCTAGGCTCG440.11302337528898021No Hit
CATATAGGGGGGAATTCATTTGATCGTAACTCGGGTGACCAATGAC440.11302337528898021No Hit
CCTTAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA440.11302337528898021No Hit
TTACTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA440.11302337528898021No Hit
TGCAAGGGGGGAGAGACCTCAGCTATTGTCTCTCAGGAATGTATGA440.11302337528898021No Hit
CGCTTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCC430.11045466221423067No Hit
ACACTGGGGGACTCTGGCACTATGGTCATGGCGGAGGGGACGGCAG430.11045466221423067No Hit
CTTACCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCC430.11045466221423067No Hit
CAAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC430.11045466221423067No Hit
TGTCAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA420.10788594913948113No Hit
CTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA420.10788594913948113No Hit
AATCCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAC420.10788594913948113No Hit
AACCAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC420.10788594913948113No Hit
GATACTGGGTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAA410.10531723606473156No Hit
TGCCGAGGGCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCAC410.10531723606473156No Hit
CACGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA410.10531723606473156No Hit
AACTTTGGGATAGAAAATTGTATTATGCATGCCGTTGCATTCGCTG410.10531723606473156No Hit
TATGCTAGGGGGCACTTCTCGGATCCTCAGCCCAGTGCTTCTAGAT400.10274852298998202No Hit
TTCACTGGGGAGAGGAGAGGCAGATGTCACGGACACGAGGACGGTA400.10274852298998202No Hit
CATTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA400.10274852298998202No Hit
ACGCTAGGGACGGCGCGGGCAACATGAATGCCACGAGCGGCATCAG390.10017980991523247No Hit
ACTTAAGGGCGCGATGGGGTACAAAAAGGACTACGATATGGTATAA390.10017980991523247No Hit
CTAATTGGGAAGTGGAGATGGCGGAGCTGTACGTGAAGCCCGGCAA390.10017980991523247No Hit
GTTTCGGGAGAACGGAGAAAGAACAACAAAGACATATTTTCCAGTT390.10017980991523247No Hit
GCCACAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCC390.10017980991523247No Hit
ATATTGGGAGAGTGAACCGCGGAGAAGGTGAAGATGGCTGCCGCCC390.10017980991523247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)