FastQCFastQC Report
Sat 31 Dec 2022
SRR5843422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21489
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAAGGGGGGACACGAGCCGGAGGCAGAGCGGGTCCACAGGCCGC300.13960631020522127No Hit
CATCTAGGGGGCCTTTGCATCCGGAGACGCGGGCTGTTCGCGTGGT300.13960631020522127No Hit
TGAAATGGGGACGGAAGTGACTCCGAACAGGAAGAGGACGAGAAAA290.1349527665317139No Hit
CGTCAAGGGCTTTTCTCTCTTTCCCCGGCCTGGCTTCCCGGCGCGG280.13029922285820653No Hit
TTTAAGGGGGCGCTGCAGCCCGGAGCTGCGGTCACCGCCGGGTCTC270.12564567918469913No Hit
TAACAAGGGGGAGTGTTCCCCAGACAGGCCACTCCACACTGGTGCC260.12099213551119178No Hit
CATGCGGGGAGGCACCGCTCCTCTGATCCCGAGCCCAACTCCCAGC260.12099213551119178No Hit
TTACAGGGGGAAGTAGCCGAAAAGGCTGACAGGATTATCACAATGC260.12099213551119178No Hit
TGATCTGGGACACTGCAGGACCGCTAGACGGACGACAACTTCTGCT260.12099213551119178No Hit
ACTTCCGGAAGCTAGCTGCGGCCTGAACAGTAGTGCTGGTGATTGC250.1163385918376844No Hit
ACAATTGGGGTGGCACCGAGTCCGCTTGCTACTTGACTGAGACTCG250.1163385918376844No Hit
AAGACAGGGATCTCCTCCAATTGGTGAGGGAAATCTCACCATCTTA250.1163385918376844No Hit
AACTATAGGGGCGGTGCAAAGAGGTCGGTGCGCAGCTCGCATCACG250.1163385918376844No Hit
TTCTATGGGGATTAGGGAGAGAGTGTGAAGAGAATGCAAAAAGGAA250.1163385918376844No Hit
CTAGTGGGGATTCCTTTAAAGCCAAAATGCTGACTACCTGCTTCTG250.1163385918376844No Hit
TTCGCCGGGTTATCTTAGTTCCAGAACTGAGTCTTCCTTTGGAGTA250.1163385918376844No Hit
ACTAATGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCT240.11168504816417703No Hit
TCAACTGGGGGTTCAGGAGCTGCACTGAATTGTGAGGTCTGTGTGT240.11168504816417703No Hit
ACACATGGGACTGCAGGACCGCTAGACGGACGACAACTTCTGCTCT240.11168504816417703No Hit
CTGATAGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTG240.11168504816417703No Hit
TTATAAGGGGGTCGCAGGCTGGGCGCACGCGAGGCTAACCGCGCGG240.11168504816417703No Hit
ATTGCAGGGGACTGCAGGACCGCTAGACGGACGACAACTTCTGCTC240.11168504816417703No Hit
AGCTCCGGGTTATCAATCTGAACTACAAAGATTTGAACGGCAATGT240.11168504816417703No Hit
TCTCTGGGGGAGACGCGCGAGCGCAGGCAAGCGGCTGCAGATTGCG240.11168504816417703No Hit
CTTGTAGGGGACACTGCAGGACCGCTAGACGGACGACAACTTCTGC240.11168504816417703No Hit
ACGTAAGGGAGCTTACACTTTCATGTTGAGCTTGGAACCTGTCACG230.10703150449066964No Hit
ACACGAGGCCCTGGAAGCCCAAGTGTTGATTCTTCGTCAGTGGTAA230.10703150449066964No Hit
TAACATGGGGACGCTCTTCCGATCTTCTGCCGGGGGGAAGCGGACG230.10703150449066964No Hit
TATAATGGGAGTCATGGCGGAGCCTTGGGCCGGGCAGTTTCTGCAA230.10703150449066964No Hit
TACCCTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTG230.10703150449066964No Hit
CAACCCGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTG230.10703150449066964No Hit
TGGTCTGGGGGGGATCCCAGCGATCGGAAAGCCGTTACTGTTACCA230.10703150449066964No Hit
CCGCGTGGGGCTCACTGCTGCGGCTGCGCCTCCTTGTTCTCAGCGT230.10703150449066964No Hit
TTTGGTGGGGTCTCTGCGCTGATGCCAGAATGTATGGTGTTCTCCC230.10703150449066964No Hit
TTTCTTGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCT230.10703150449066964No Hit
TCAAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGT230.10703150449066964No Hit
TTTCTCGGGGGAGAGGAAAAGTGAGCTGTGCTGTGGCTCCCGCGGC230.10703150449066964No Hit
GTCAACGGGTCAGATGAAAGTTCTGTTGATAGAAATCCTTCAAAAG230.10703150449066964No Hit
CGATAAGGGCGGGAGGCGCAGAGATAAAGGAGAGTGCTTCACAGAC220.10237796081716227No Hit
CATCGTGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTG220.10237796081716227No Hit
ATTACTGGGGAGTTCCAAATGACCTTCCACCTGTTTATTGCTGCGT220.10237796081716227No Hit
GTCACAGGGTGTCAGGCTCTGGGGGCTAGGCAGGACACACAGTACA220.10237796081716227No Hit
AATTAAGGGGGGACAGAATTAGAGATAATGAAAACAGAGTCCCTAC220.10237796081716227No Hit
ACATGAGGGGGTCGCAGCAGTGCTCGCAGCCGCTCTCTCCCCATCT220.10237796081716227No Hit
CGATGCGGGATGTATTTCGGGGTCGGGGGACAGGAGTGCGTATCCG220.10237796081716227No Hit
GCCAGGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTGC220.10237796081716227No Hit
TAGCTTGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCT220.10237796081716227No Hit
GACCACGGGACACACACTGCAGGACCGCTAGACGGACGACAACTTC220.10237796081716227No Hit
CATGCTGGGGACCCTTGAAGTATGTCCTAAGAATTTGCCTCTTGGA220.10237796081716227No Hit
AACAGTGGGAGCCCTGTCTCGGATCGCTGGTCTCTGCAATCGTGCT220.10237796081716227No Hit
TTTCATGGGACACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCC220.10237796081716227No Hit
GATGAAGGGGACCCCAATCCAATGCACATCGATGCCACCTTCAACA220.10237796081716227No Hit
CAGTGGGGGACACACTGCAGGACCGCTAGACGGACGACAACTTCTG220.10237796081716227No Hit
ACTTAAGGGGTAGATTTCAGTACTGCTGGCTAGCAGCTTTATCCTC220.10237796081716227No Hit
TTTCGAGGGGCGGGGAGAAGCGGGCACGCCCGCGCAGACGAGGCTG220.10237796081716227No Hit
GCGCATGGGGAGAGAGAAAAAGTAAAGGACAGAAGAAGGAGACTGG220.10237796081716227No Hit
TATCCTCGGGGCGGTCCGAGCGAGCGCGTCCTTGCTTCAAAGAGAT220.10237796081716227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)