FastQCFastQC Report
Sat 31 Dec 2022
SRR5843391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25301
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC390.1541441049760879No Hit
ACGCTTGGGGTGCCCAAAGAAGAACTGCAGGACAGGCTGGTGGTGG350.13833445318366863No Hit
ACAATTGGGGAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGT340.1343820402355638No Hit
TTACCAGGGAGGCTGCGCGCCCGGTAGCCACCGCTGCGCTGTGAAC340.1343820402355638No Hit
TTTTCTGGGGCACAGTTGTTTGGGAAGCGCGACAGCATGGACGACG340.1343820402355638No Hit
TAACCAGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAAC340.1343820402355638No Hit
CACGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC330.130429627287459No Hit
GCCTCTGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG330.130429627287459No Hit
GTTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT330.130429627287459No Hit
CTAGTAGGGCAGTGCAGATATATTACCGACAAAAGGAGGCAGCTGC330.130429627287459No Hit
GATCTTGGGCCCACCCCCGGGAGGTGCCCATCCTGATCTGGTTCTT320.1264772143393542No Hit
GATTTTGGGGACTCTTCTTCAGTCTGCTTCTTTTTTCAGGCAGGAC320.1264772143393542No Hit
CCACATGGGCGAGTGTAACGGGGGCCGTGGTACCTGCCACTACTTC310.12252480139124935No Hit
GTACCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT310.12252480139124935No Hit
CGAAGGGGAAAGAAAAAGGGGGAAGGAACCAATCAGGAAGCCAGAT310.12252480139124935No Hit
TCATATGGGGGGCAGCTGTGCTGCAGAGACAAAAAGTGGTGCTGAG300.11857238844314455No Hit
TGCAAGGGGGCGGCGGCACAGAGACGAGCGCGGGTGGAGGGCGCGG300.11857238844314455No Hit
ACATGCGGGGAGTGGCAGTGAGTTGGGTCTTGGCTCCTAAGTGAGA300.11857238844314455No Hit
TCGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT300.11857238844314455No Hit
GTACTTGGGGGACGAGGCGACATGGCGGCGGCGCTGCTGCTGGCGC300.11857238844314455No Hit
CTGATGGGGAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTA300.11857238844314455No Hit
AAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTG300.11857238844314455No Hit
AATCTACGGGGCGGTCCGAGCGAGCGCGTCCTTGCTTCAAAGAGAT300.11857238844314455No Hit
AATAGCGGGGGAGCTCCAGAAATGGCTACCGCGATGACAGTTTCCA300.11857238844314455No Hit
CATGCCGGGGACTGACGGAAGTTAGAAGATGGCGGCGTCCGCAGTC300.11857238844314455No Hit
CATATGGGGGGTGGCAAGGTCACACTGAGCTCATGTGGGTGAGGAG290.11461997549503972No Hit
CACGAAGGGGCATCCTTGAAGTGTCTGGAAGGATGCTCAGCCTTGC290.11461997549503972No Hit
CAAGCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGTCTTTCTG290.11461997549503972No Hit
TGTGTTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACA290.11461997549503972No Hit
CTTATCGGGGAAGAGTCCGTTCTAAAACTTTCTTTCAGCAGGTTTG290.11461997549503972No Hit
ATAAGTGGGCAATGTTATCGTAACTGCCCATGCTTTTGTTATAATT280.1106675625469349No Hit
TGTTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC280.1106675625469349No Hit
TGGCCTGGGTCTCTGAGGCGCCTGAGTGAAAAGTGTGGCACCATGG280.1106675625469349No Hit
CATCTTGGGGTTGATTTTTATCATTAGCCAGCTGAGCCTGCAGCAC280.1106675625469349No Hit
TAAATCTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAAC280.1106675625469349No Hit
TGCGAAAGGGACAGTTTCGGGACCCCGGCCCTTCCAGACGTTCGTG280.1106675625469349No Hit
GTCTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC280.1106675625469349No Hit
AATCTGGGGATCGTTACTTTTGGGGAGTCTTCGAGGGGACCAGAAA280.1106675625469349No Hit
CGCATAGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA280.1106675625469349No Hit
TCTAGCGGGGAGGCGACATGGCGGCGGCGCTGCTGCTGGCGCTGGC270.10671514959883009No Hit
ACCTCAGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG270.10671514959883009No Hit
GCCAACGGGGACGAGGCGACATGGCGGCGGCGCTGCTGCTGGCGCT270.10671514959883009No Hit
CCATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT270.10671514959883009No Hit
TTTGCAGGGCGATCTATCAGAGGGGTTCCCTTTCTTTGTCTTCTCT270.10671514959883009No Hit
ATCTAGGGGGCTTTTTTCCCCCTTAGCGCGGCCGACTCTGCAGGTC270.10671514959883009No Hit
GGATAAGGGGACGAGGTGAAGGAGTCGAAGCGCATCAACGCGGAGA270.10671514959883009No Hit
TTCTATGGGAGGCGAGTAGCTAGCGCTCAGGCTCGGCAAGGAGCAA270.10671514959883009No Hit
ATCTCAGGGCAGTATGAATTTGTGCACCATGCTCTGTGCCTGTTCG270.10671514959883009No Hit
ACCGTGGACTTTATTTAATTTCATAAATTGGCTCCTTTCCCACAGT270.10671514959883009No Hit
GCTAAAGGGGACTCGTTTGAAGTCTTCCTCTGCCCTGTGTAGTCTG270.10671514959883009No Hit
AAGCGCAGGGGGTCCGAGCGAGCGCGTCCTTGCTTCAAAGAGATAG260.10276273665072526No Hit
ACGCTCGGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACA260.10276273665072526No Hit
TGAAAAGGGAATTTTATTTTTCTCAGTGTTCTGGACACTGTAGACC260.10276273665072526No Hit
TTTGCTGGGGTGTGGAAGACCTGAACATCATCCAGGTGACAATTCC260.10276273665072526No Hit
TTCTCTGGGGAGCGGGAAAAGCACTTTCAGAAGGAGGAACAGGAGA260.10276273665072526No Hit
AAGCTGGGGCAGCCTCCAGCAGCTACAAGACATGGAACCTGCGTGC260.10276273665072526No Hit
TTTCGCGGGGGCCGCCACCCTACCATGAGTCCTTGGCTGGAGCCAG260.10276273665072526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)