FastQCFastQC Report
Sat 31 Dec 2022
SRR5843281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30413
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAAGGGTGGGGAAGACGATGGACAATCGGCACCAGTCAGAGCG390.12823463650412653No Hit
ATAATAGGGAACCCTGCTGATGCTCCCAATCCGGTGGTATTTGTGG380.12494656890145661No Hit
CAGTCGGGGGAAGAGAAAGATCCAAAAGCAAGAGGCAAAAAAGTTA350.11508236609344688No Hit
ATTCCCGGGGCACTGCCCCAAGGTGAAGAACTGATTGAAGCAGCCA330.10850623088810706No Hit
TTTTGCGGGGTACCCAGGTGGCCACAGCAAGTGATACGACCCTCCG330.10850623088810706No Hit
AAGCAAGGGCCATCTCGCTGGCCCTGAGGGGAGCCAAGACCTTGTC320.10521816328543715No Hit
GAACATGGGACCATGGGACATGTTTTCTCCACGGAAAAGCACAATT320.10521816328543715No Hit
GCTCCCGGCCATATAACCAGCGCTCAGCTGCCTCTTTTCTTTGCTT320.10521816328543715No Hit
CATAAAGGGCAGCAGGTTCAGCAGAGTTTGGAAAAGATTTCTAAGC320.10521816328543715No Hit
ACCAAGGGGCGATCTCAGTATGGGGGGAATTCACACCGGCGCACGC310.10193009568276723No Hit
CATCTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT310.10193009568276723No Hit
CTCTCTGGGGTGTGCAGAGCAAGAACAGCAGTTACTTCGTTGAGTG310.10193009568276723No Hit
TGACCTGGGATCCCTGTCCACCCAGATCCCCAAGAACCATGTCAGT310.10193009568276723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)