FastQCFastQC Report
Sat 31 Dec 2022
SRR5843245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53865
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCACACCCTGAGTGCAGGAACTGGGGAGGGTCCTGCTGGTGGG850.1578019121878771No Hit
CAAACTGGGCAGAAAGCATCACAAAAATATTTGAAATTGTACGGTC700.1299545159194282No Hit
TGCAGGGGCGCAGACGCTTGAAACGATGTGACATCGCTTCTAGAGT670.12438503666573843No Hit
ACACCAGAGATGCACAGGGGCCTGTTCTCACATCCCCAAGCTTAGA650.1206720504966119No Hit
ACGTAGGGGGGACCCTGAGAAAGCCAGAGCCGGTTTGCAGGCAACG640.11881555741204863No Hit
GTTAAAGGGGAGCCGGGTGAAGCCCATCCGGGCAAGCCTTCCCTGG620.11510257124292213No Hit
CCTTCAGGGGGAACAACCAGCGACTGCGTGAGCGGGGCTGTGGATC610.11324607815835885No Hit
TTTCACGGGGGCTGAACCGCTGTGGCTCAGAGTCCCTCGGCTAGCT610.11324607815835885No Hit
CTGCTAGGGGACGCGTCCCGGGTCGCGCCGGCCCAAGATGGCGTGG600.1113895850737956No Hit
CTTGAATGGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTAC600.1113895850737956No Hit
GAGAACGGGGCAGTTCGGCGCGCGCAAGTCGCCGGGAGCTGTAGTC600.1113895850737956No Hit
CAAATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA590.10953309198923233No Hit
CAAAATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC580.10767659890466909No Hit
AAATAGGGGGAGGGTGTATATCTCTCAGACCAGCAATCGTGGTTCC580.10767659890466909No Hit
GCATAAGGGGACATGGAACGAGGTCTAACTGAATAATGGCTATGTT580.10767659890466909No Hit
CCAGGTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC580.10767659890466909No Hit
TTCAATGGGAGGTGGCACCAACCTTGACTTTCAGATGGAGCAGTAC570.10582010582010583No Hit
CGACTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAA560.10396361273554255No Hit
CAACCAGGTGCACTTTTAGGAGATGACCAAATTTACAATGTTATCG540.10025062656641603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)