FastQCFastQC Report
Sat 31 Dec 2022
SRR5843235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50993
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCGGGTCCAGTGGATTTTTTTAACTATTATTCAAAAGCAAAA810.15884533171219578No Hit
CGCTTGGGGGTGGTGATCCCTTCCGCTTTACCCAGGCTGGCTCAGA740.1451179573666974No Hit
GTTATGGGCTGTTTAGCCTGGCCTTTCAGGTCTGAGAGAACACTAA740.1451179573666974No Hit
TATGCTTGGGATGAACAGATCTGGCCAACACAAGAACCTGTGTCTT690.13531268997705567No Hit
ATCGTGGGGAGCCTCGGGGCTCACAGAACAGAGGGGCTGAGTTCAT680.13335163649912735No Hit
GCTTCACCTGCTCCCACTGAGGGTCCCCGGCCCTCAAGACCTTCTG670.131390583021199No Hit
CTCTAAGGGGCCACTGTGCCTGCAACCTTGTCTGAGAGGAACCCTT640.12550742258741396No Hit
ACGTATGGGAGCACAAGAGTCACCTGTACCTACTGGTCACTGACCA630.1235463691094856No Hit
CACTTTGGGTGAGCACAAGAAGCAGTAAGTTGGCACCTTGCCACAC630.1235463691094856No Hit
AGCTCAGGGCAGATACCAACACCTCTGGAATATAAAGCTGCAGTTG610.11962426215362894No Hit
GTATTTGGGGATGAATGGGAGGAACTGGTAACTTGGAAGGAAATGA610.11962426215362894No Hit
GAAATAGGGGACTTGGAACGAGCGCGGGACAGATAACGGAGCAGAG610.11962426215362894No Hit
CTTACAGGGACAGTTGTTTGGGAAGCGCGACAGCATGGACGACGAG600.11766320867570058No Hit
GCCACTGGGGGGGCTTGCAGGCTCTGAGGGCATGTCGCAGGCGAGC590.11570215519777224No Hit
CAACCATAGTGGCCTCGTGACCTTCCAAGCCTTCATTGACTTCATG580.11374110171984389No Hit
CTATGCAGGGGGCGGCGGCGGCAGCGTAGTTCCTAGCCGTGCAGCT580.11374110171984389No Hit
TACCAAGGGGAGTGCCGAGCTGCCGATCGGGTTTCCCGGCAGGCGC570.11178004824191556No Hit
TTGATGGGGGTTTGTCTTCATTAAACTATAACATCCAGTGGCAGAA570.11178004824191556No Hit
CAGCCGGGGGACTGGGGTTGCCTTCTGATGGCGGTCACTTCATGGG570.11178004824191556No Hit
TCCCTGGGGGACGGACCTAAGATGGCGGCCTCCAGGGGGCTGGGAA570.11178004824191556No Hit
GTATATGGGGGACCATGGAGACTGTGGCCCAGTAGAGACCTTAGTG570.11178004824191556No Hit
CGAACAGGGGAGATCTAGGTGGCCGGGCGCGGAGCCCAAGCCGTGC560.10981899476398722No Hit
AACCACGGGGGTGCCTGCAACCTTGTCTGAGAGGAACCCTTCTCTG560.10981899476398722No Hit
CTATCTGGGGGAGTTTGGCTTCGTGTCCTGGGTTCTTCCCCGTCCC560.10981899476398722No Hit
TCGTACGGGGGACAACAACTCCCACAAGAAGAACATCTTCGAGAAA560.10981899476398722No Hit
TCCACCGGGATCATAGAAAATTGTATTATGCATGCCGTTGCATTCG550.10785794128605887No Hit
TGCTCAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC550.10785794128605887No Hit
CATTGCGGGGACACCTAGGTATGCTGTGACAGGTGAACACTGCGCA550.10785794128605887No Hit
GGCGCTGTGTCTCCTCACTATTGTCGCCCTGATTCTGCCCAGCAGA550.10785794128605887No Hit
TAGTGCGGGGTCACTGGGAGCAAGTACACAGAGTTCAACTCTTCCA540.10589688780813053No Hit
ACGATGGGGGGCCCCACTGCTTCGACCTCTGCCGCCGCCACTGCCG540.10589688780813053No Hit
CACATAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC540.10589688780813053No Hit
CTTATCGGGGGCAGTATGAGGAGGTCCTGTTTTTACAGTAGTGTTT530.10393583433020218No Hit
CTTGTTGGGCTGTCCCTGCTGGTCCTGGGGCTGCCAGAATTCCCTT530.10393583433020218No Hit
TCTCGCAGGATACTAAGAAGAATTCATTCAAGAGAAAAAGCATAAT530.10393583433020218No Hit
TTAGATGGGGGGCAGGCGCAGGCGGAAAAAGCGAGCACCGAGCTTC530.10393583433020218No Hit
GCAAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGG530.10393583433020218No Hit
CCGCATGGGGGCCACTATGTGCTACGTAAAGTGTGCAAGATACATC530.10393583433020218No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATC530.10393583433020218No Hit
TGGGGAGATCCATCCAAGGGGTTCAGCCCTCTCCTGCCTCCATGCC530.10393583433020218No Hit
AGCAGAGGGGGGCTCTCCGGAGGGAGCGAGTGGTCAGTGCGGAGCC530.10393583433020218No Hit
TTTTATGGGGTAAAAGCAAAGATGGAAAAAAAGTCCACTCCTTCCC530.10393583433020218No Hit
ATACGGGGGGCGCGCCATCTTGGCTACTCCGTGAGGGTCGAGCGTC530.10393583433020218No Hit
TTTAAGGGGGATAGAAAATTGTATTATGCATGCCGTTGCATTCGCT520.10197478085227385No Hit
TTGCTAGGGGGGAGAAGATTGAAAAGGAGCTGGAGACAGTTTGCAA520.10197478085227385No Hit
CGGCATGGGGGGAATTCGAGCTCTTTCGACGTTTTGAAGGAGGGTT510.1000137273743455No Hit
GCCTAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCT510.1000137273743455No Hit
AATCCAGGGTCTGTAAAAATGTGACCTCAGCTACTGCCAATAAAAC510.1000137273743455No Hit
AACATAGGGGAGGAAGCGTCCGGAAAAACCCCACAGAAGCCAGGAC510.1000137273743455No Hit
CAAAGGCTGCAGCTATCTTTTTACCAGGAATATCTTCCTATGGCTT510.1000137273743455No Hit
AGTCAAGGGGGGCCGCCCGGGACGGACGTGGAAGATGGCGGACGCG510.1000137273743455No Hit
CCTCCCCCTCAGCCTATGGAGATCTTCGTGGATGACGAGACCAAGT510.1000137273743455No Hit
CGCCCTGGAAGAGACCTGGAGAAACCTCCAGAAGATCATTAAGGAA510.1000137273743455No Hit
GCAGCCGGGGCTGGAGGAGGGGACCCAATTCTCTACCAGCATCTGT510.1000137273743455No Hit
TATTTAGGGGACGTAGATGTTGGAAAGAAGAAAGCAGACATCTCGT510.1000137273743455No Hit
AAGCCTGGGTCCACATGCAGTCCTTTGCTCAGGAAGTCTTTGGGGA510.1000137273743455No Hit
CATAAAGGGGACACACGAGCTTACTTTACATCAGCCACTATAATTA510.1000137273743455No Hit
CTTCCCGGGGCAGTTTGTCTTCGCTGATCGGTGAGACTTCGAGCAG510.1000137273743455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)