FastQCFastQC Report
Sat 31 Dec 2022
SRR5843231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCACACCCTGAGTGCAGGAACTGGGGAGGGTCCTGCTGGTGGG1050.21No Hit
CTGTCAGGGGTGGACAGGTGAAGAGACAAAGAACTCCTTTGACTCC740.148No Hit
CTTAGCGGGGATCTTCTGAAGGGGTACACGTATACACACACACACA720.14400000000000002No Hit
CGTACAGGGACCAAGGGATAGAACAGACAACCAAGAGACACACTTG710.14200000000000002No Hit
AGAAGCGGGACCTTCTCCCAGCGCAGCCACAGCATCTTTGATTGTC680.136No Hit
AACTAGGGGATTCGTTTGAAGATAACTACTCCTATGAACTCTCGAG670.134No Hit
CCACTTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT650.13No Hit
ATGAGTGGGATATCTATCAGGTAATATAGGCTTGAAAATTGATATC640.128No Hit
TCAAATGGGAATCCATGCACTGGGAGATGGAAACAGACAGTGGCCT640.128No Hit
AACTCTGGGATCAGTCAGTAGCTAAGAGTACTTCCCACCTTAGTTT640.128No Hit
TCTATTGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG630.126No Hit
AATGAGGGGATGAGGGAATTCTGAGCTGAGATTGTCCTTGTCACCA630.126No Hit
GCTAGTGGGAAGCGGAGATTTCCTGAGGCACTGATGCGTTCTGGGG630.126No Hit
GCCTAGGGGGTTTTCTTTAGGATATAAAGAGAGCCTTAGAGTGCGT630.126No Hit
TTTTGCGGGGCACATACCCCAGCCTCTTTATTGAGGTGCTAGATGT620.124No Hit
TAGTTCGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTAGA620.124No Hit
TATCTCGGGGGTGCACCAAAGCTTCTTTTCGTACCAGGACCTGTTG600.12No Hit
AGCTTGGGGTGCGAATTGGAGCGATGTTGGCCTACACGCCTCTAGA600.12No Hit
GGACAAGGGGAAAGTTGAGGAAAGCAGTCAAGGGCAGGCTGGGATC590.11800000000000001No Hit
TGTAACGGGGAACAATTTTTTTTCCAGTTTGTTTGAAAGGGACTGC590.11800000000000001No Hit
TGCAATGGGATGTGCTTGTCCTGATCAAAGCATAAAGGGCAGAATT590.11800000000000001No Hit
TCAGCACGGGATCCACTTCCCTCTGGCCACTTATGCCCCTGTCATC590.11800000000000001No Hit
CAAGATGGGATTCCCTTCCAACTATAGACATCTGAAGAGGACTGAG590.11800000000000001No Hit
CTTTGAGGGGGAAGTGGAGATGGCGGAGCTGTACGTGAAGCCCGGT580.116No Hit
TCATAAGGGGACTTCCGGAAGTGCTGCGCAGGGTTCCGGGAGAGGG580.116No Hit
TCTTAGGGGGGATCCCCGCGGGGCGGTCCTGTCTGCCGAGACTGGG580.116No Hit
ATGCAGGGGGGCACAGACACCAAGGACAGAGACGCTGGCTAGGCCG580.116No Hit
TAATTTGGGGCTGAGTCTATGGGGGATCTGGAGCAGGTGCAGAGCC580.116No Hit
AAATAGGGGGGTCCACATTTTTTTTTATTCTTTTCTGTAGTGCTAC580.116No Hit
CGTCTTGGGGCTTGGCAGGATTCATGGGTGCCCACTGTGAGGTCAA570.11399999999999999No Hit
TCTTGGGGAAATTGGCCGCGGGTGAGGAATTCAGGGCTTCCCGTGT570.11399999999999999No Hit
ATATTTGGGATTGTCAGGAGATCAGCAGTTCAGTCCAGTAGCAAAC570.11399999999999999No Hit
TATTATGGGATGGAAGACGTGTTCTTGCCAGCTTCCTTTTGTCTCA570.11399999999999999No Hit
TAAATCGGGAAGCAGAGAGTTAAAAGATGGGCTGTCTGGATTTTTA570.11399999999999999No Hit
TGTCAGGGGATGGTGTGAAAGTTGGCCTGACAAATTATGCTGCAGC560.11199999999999999No Hit
ACTAAGGGGGCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCC560.11199999999999999No Hit
TCTCGCGGGATTGTGCATCAGGCTGGCATGTTGAAGCGAAATTGTT560.11199999999999999No Hit
CGCTGAGGGGAGACTTGATTAAAGTCAGGGTGTCCTTTCACTGGGT560.11199999999999999No Hit
TACTACGGGACATTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTT560.11199999999999999No Hit
TGAAATGGGACCTGTAACTTTTTAGGAGTCTATAAAATAACTACCA550.11No Hit
TTAGCGGGGGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTATC550.11No Hit
CAACAAGGGATTCCTCAGAAGGGAGGGTAAGGAGGGGTGTCTCTGT540.108No Hit
TCTTTTGGGATCAATCACATGTATTTCAGAAAGGCTTGGCCCCCAT540.108No Hit
CTGAAAGGGGACTTCCTGGATGAGCTGGCAGAGAAACATGGCATTG540.108No Hit
GCCGCAGGGGATTTAAAACCAGCTGGAGCAGACCTCGGCTCCACCA540.108No Hit
TCTTTCGGGGGCTTGAATCGGGTGCTGGGCCTGGGCCTGGGCGGCC540.108No Hit
AATTACGGGATAAGGAAGCTGAGAAGAGCCCACTGCTGGGGTTGGG540.108No Hit
CCACTGGGGATGTGACTGTGCAAAATGCAATTTGCCCATTTCCGTG530.106No Hit
GCTCAAGGGGAACACGCAAAATACACGCAGCATTGCCCCTTCGATG530.106No Hit
CTTCTAGGGGATTCTGAAAGGTCATCTGAAGCCTGAAACCCCTAGA530.106No Hit
ACTTGAGGGGGGAGACTGAGGCCAGAAGGCCCAAGAACAGATGGCA530.106No Hit
CTCCTTGGGACCGTGGCATCTTCCCTGTGCTGTGTAAGGATGCCGT520.104No Hit
ATTACCGGGGTTCAGAATCAGTTCTTAGGGATGAATTTCATCTTCA520.104No Hit
TCACAAGGGAATTTACTGTATTTTAGTACCAATGGCAGGAAAACTG510.10200000000000001No Hit
TTCTGGGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC510.10200000000000001No Hit
TACTCTGGGGTAGTGTCTGCAGCACATAAATAAAACACTACTCTCC510.10200000000000001No Hit
TTTGACCGGGGTGTTGTAAGCAAGAAGTCCATTTTTAACAAATTGA510.10200000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)