FastQCFastQC Report
Sat 31 Dec 2022
SRR5843213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45588
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATATGGGGACTTTGGCTGGGCACTCTCAGAGCCAGGGTCCTTCC640.14038782135649733No Hit
CAGTAGGGGAGGAAGCGGAAGGGAAAAGATGGCGGCGCCGAGTGTC570.12503290339563042No Hit
TTAGCAGGGACCCTCGGTCCGGGACAAGTTTATCTTGTGACTGCTC550.1206457839782399No Hit
TTCATTGGGGGGGGAGAATCCGCGTTGAAGAACAGAGCATTTAGCT550.1206457839782399No Hit
CGTCATGGGGGGAGCCGGAGTCCGGGAGTGCTGCTCCCTGAGTGTG550.1206457839782399No Hit
CGAAACGGGCGATCTGAATGGGGGGGGCTGCGGCGGTCCGACTGCG550.1206457839782399No Hit
GAGTACGGGAGGTGCGACATTGCCGACGCCAATTTCAGTCCGCCAG550.1206457839782399No Hit
TAACAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT540.11845222426954462No Hit
CACTACAGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACA540.11845222426954462No Hit
GCGAATGGGAGCTCAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTC530.11625866456084935No Hit
TTCTATGGGGATAGACATCCCTCAGGCTTTGGTTGCTGGGGACTCT530.11625866456084935No Hit
GTTCCAGGGAAGTTCAGAGTTGCCTTTTTCTAGTGAACTCAAGGGC520.11406510485215408No Hit
TATCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG520.11406510485215408No Hit
ATCTAAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT520.11406510485215408No Hit
CTTAGTCGGGGGCGGTTTCTGGGTTTCAGAGCAGTGGCCTGGACTG510.1118715451434588No Hit
CATTGCGGGCTTTCGCGCTACCCGGGGACGGGTCCATACGGCGTTG510.1118715451434588No Hit
CTTGTTGGGGGTCTGGCCCTTCCAGGAATCTGGAGTGCAGTAGAGG510.1118715451434588No Hit
GGACTTGGGCTGCATGAAGCAACGATTAAAAAAGAAAATCACAAAA510.1118715451434588No Hit
CTTTAGGGGGGGCGGAAGTTCCCTAAGCTTCCGGCCGCCGGCTTGG510.1118715451434588No Hit
GAGCCAGGGGGTGTTCGCAGCCTTCGCCGCGCCGCTGCCGCTCTCC500.10967798543476354No Hit
CTACCTGGGGAAAAATGAAAAGTATCTCGGCTTTGGAACACCATCT500.10967798543476354No Hit
TTCATTGGGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGTTCA500.10967798543476354No Hit
GCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTG500.10967798543476354No Hit
TTAGAAGGGGGAGCAGGATGGCGGCTTCAGGCGAGGCGCGCCGGGT500.10967798543476354No Hit
AACGATGGGGCTCTGTTTCTCCGTGGCCGTGATCCTTGTGGCTGGC500.10967798543476354No Hit
CACTGCCTACCACGACATCTCCCTAGACAAGTGCTACGTCATCGAG500.10967798543476354No Hit
TGGTGTGGGGCTGTTCCCGGAGCGGATCACCGTGCTGGACCAGCAC500.10967798543476354No Hit
GAATTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC500.10967798543476354No Hit
TTAACTGGGGAAGGGGCAGGGATGAGGTGGATAAGATACAAGTAAG500.10967798543476354No Hit
CTACTAGGGGTGCAAGATGGCTGCGGCAGTTCGGAGCGTGAAGGGC490.10748442572606827No Hit
GTTACAGGGCAGAGGCCGAGGTCGTGGCCGAGGGAGAGGTGCCCTC490.10748442572606827No Hit
TTCTTAGGGGATGTTAACAAACCTGGCTGTGAAGTTGATGACCTTA490.10748442572606827No Hit
ATCCATGGGGTGTATCGCGAATGCTGGCCCATCCCTTACACGTGAT490.10748442572606827No Hit
GATCAGGGGCGCTGCACTGAGCCGCTGCTGCTGTCGCGCAGTCCGC490.10748442572606827No Hit
TATAGTGGGGCTCCTTCCCCAGCCGCCTTCAGGACAGCCGCAGCCT490.10748442572606827No Hit
TTCTCGGGGGATGGAACTCAAGAATCCTGTGAGTCCCCTGATGTCC490.10748442572606827No Hit
TTAGCTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCT480.10529086601737299No Hit
CATATCGGGGAGGGAAGCTGCGAGCCTCGAAGTGCGTTTGGGCTCG480.10529086601737299No Hit
CTATTCGGGGGACTGCAGGACCGCTAGACGGACGACAACTTCTGCT480.10529086601737299No Hit
CATATAGGGAGGGAAGCTGCGAGCCTCGAAGTGCGTTTGGGCTCGC480.10529086601737299No Hit
GACGCTGGGATTACATAGGTGTCTCTCTTGGCAGCATCATAGCTAT480.10529086601737299No Hit
CAAAATGGGGTTGTAGGAGGGTTGTAGATCATCATGTTGCCATCCT470.10309730630867772No Hit
CAGCCCGGCGGCCGAAGCTGTCAGCGGGGAGGCACGATTTGCTTAT470.10309730630867772No Hit
CAATGGGGGGGAATTCGAGCTCTTTCGACGTTTTGAAGGAGGGTTT470.10309730630867772No Hit
TGCACAGGGGAGCTTGGCACCGCCCAGGCCGCGGGACCGCGCCCCA470.10309730630867772No Hit
TATCGAGGGATCCCCGAAGGGGCTAAGATCGCCCAAAATGTCATCC470.10309730630867772No Hit
TCTCAGCGGGCAACCAAATAGGACCAATGGAGATAAGCTACGAAGA470.10309730630867772No Hit
TCGCCAGGGTAACAGAAATGCAGTGTCTCATTTTGCTGGGTAAGAG470.10309730630867772No Hit
CAACATGGGATGGCAGAGTACTACGAGAGCTCTAATTGTGCCATCC460.10090374659998247No Hit
TTTAAGGGGGAATGGAAGGCAAAGAAGAGCCTGGTTCTGACAGTGG460.10090374659998247No Hit
TGTCTAGGGGCCGGACGCGGTGCAGACCATGACCCAACAGGGCGCG460.10090374659998247No Hit
TTGCTTGGGGTGAAGAGTGATGGCTGACAGGTCCTCTGTATGTTTC460.10090374659998247No Hit
GAACATGGGGCTTTTAGGTGTAAGCCGCAAACATCTTGGACCCCGG460.10090374659998247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)