FastQCFastQC Report
Sat 31 Dec 2022
SRR5843167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60501
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTTGGGGCAATGCTTGCAGTATGTATCCTGATCGTGCGTGCGA890.1471050065288177No Hit
TAATCTGGGGGAATGAGCATCCCAGCAGAGTGACAGAGCTCCCTTT830.13718781507743674No Hit
ACTCCAGGGACTCTAAGGACGCTGCCGTGTTAGTTGCCCGGCACTG820.1355349498355399No Hit
TTGTCTGGGGTAAACAGAATGCAGAAAACAAATGTTCATTTTGACA760.12561775838415895No Hit
GCTTGAGGGACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTG740.1223120279003653No Hit
AACTGTGGGGTAGAAAATGGGACTCTTGCAATCTCTGACTTTGACA740.1223120279003653No Hit
CATAGAGGGGTTTCACTGGTGCTAAGGGTTGAGAATAGCAGCGACT720.11900629741657162No Hit
GGTGCCGGGAACACATTTCAACTAACCACAACAAATGTTACATTTA700.11570056693277796No Hit
CACATAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT690.11404770169088113No Hit
TCACAGGGGATGGCACAATGACAATAAGACTTTCCTGGTGTGGATT690.11404770169088113No Hit
CATACAGGGAGGCATGGGGGCTGGTGCTAAGAGTTACATATCTATT680.1123948364489843No Hit
TCCTCTGGGAAAGACTCCCCATGCTCCTTTAGAACTGGAGGTGAAT670.11074197120708748No Hit
CTCGTCGGGATTGGGAATACTGAAATGAAGTTAGAGGAGAACAAGA660.10908910596519066No Hit
TGGCATGGGACTTCTTAAAAACGGACAACGTTTTTCTAGCCAGAAG660.10908910596519066No Hit
CAGTAGGGACCCACATTTTCCTTCAACAGTGCTGCTGCACCCAGTC660.10908910596519066No Hit
TCCAAAGGGGATCAACAAGACAGCCCATGCTTGCAGAATCCTGATG650.10743624072329383No Hit
TAATCCGGGGATCTCTTGGTGGGGCATGTGTCTCCAAGTCAGTTTG650.10743624072329383No Hit
CTATAGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACA650.10743624072329383No Hit
ACTCCAAGGGCAGTGAGGGAGAGCTTGCGAAACTGGTCTGCAAGTC650.10743624072329383No Hit
TCCACAGGGATCCGCCCAGCCGAGCCGTCCCTGCTCGACTAGGCTG640.105783375481397No Hit
AGCTTTGGGACTTGGAAAGGCTAAAAATTCTGCGGCAGCGGTGTGG640.105783375481397No Hit
AAACTCGGGGTTGCTACTTAGGACAAAACCATAAGCAGTCGGGTGA640.105783375481397No Hit
AATCACGGGGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT640.105783375481397No Hit
CAACTGGGCGATCTAAAAAAGGGGATATCTTTAACAACTACTCCTT640.105783375481397No Hit
ATCCAAGGGATTGTCACATATATCACAAATATTTTTGACACACTGT630.10413051023950018No Hit
TATTGCGGGATGCAATAACAGCCAAAGACCCTTTTATTTGTGTTTA630.10413051023950018No Hit
GCACTGGGGAGTGCCTGTGTGAAGATGTGCCACGCTGCCAGGCCCT630.10413051023950018No Hit
GATAAAGGGGTAAGACAAAGATTCTATAACCTTGTCCAACTCTATG630.10413051023950018No Hit
AAGAACGGGACCCTGAGTCAAGTGTACCCACAGTAAATGCCACTTC630.10413051023950018No Hit
ACTTTAGGGGAAGAGCTGCTGATGAGGTGGTTGAAGAAATGGCTAT620.10247764499760334No Hit
TTGCGCGGGAGGCATCTGTGTTGCACAATAGGAAGAGAAGTTGGGA620.10247764499760334No Hit
GTTACCGGGGGAGGAGGGCAAGGAGATCCTGGTAGGAGATGTGGGG620.10247764499760334No Hit
TGAATAGGGACCTAGTTCAGTGATAGAGAACAAGATGTGTAACTCT620.10247764499760334No Hit
CTCAAGGCGGGGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTA610.10082477975570651No Hit
CGTACAGGGATCGAGTGAAGGTTGTGTCTCTAGAAAGGAAGCTTGT610.10082477975570651No Hit
GTTTTTGGGCTGTTCACCCCAGGTGGTATACCCAGTTGTCTGCTAG610.10082477975570651No Hit
AGTCATGGGATGGGAGAACTGATGACTCTTGAAGGGAGGTGGATGG610.10082477975570651No Hit
AATTACGGGGTCACGACATATGCCAGGTTATCTGGCCCATGAACTC610.10082477975570651No Hit
TCTTTTGGGGACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAA610.10082477975570651No Hit
AATCGTGGGATGTCTGTCCAGTGCATCCCTCTTTTTGACTTTTCTT610.10082477975570651No Hit
GTACGAGGGATCCCGGGGACATTTGAGAATCTTTCTGTCTGGAAGC610.10082477975570651No Hit
CAGTACAAGTTGGTTCTGTGGCCTTTTCCGTCCTTCCTGATACATC610.10082477975570651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)