FastQCFastQC Report
Sat 31 Dec 2022
SRR5843130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52237
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATTGGGAACCCCAGTAACCCCTACTGTGCAGGCATCCAGGGCA1010.19334954151272088No Hit
TTGCCGGGAGGCCTGCGGGGCGGAAGAAAACCTGGGAAATCTCAGG810.15506250358940982No Hit
TATAATGGGTATGTGTGTGCATATATATGTGTGTGTCACGTTAATA680.1301759289392576No Hit
ATGTGGGGGTTGAAACTCTTCCCAAGTAGTTGGGAAAATAGCTAAG660.1263472251469265No Hit
CATTGAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATG660.1263472251469265No Hit
GCGAATGGGGTTTGAGGATGGAGACCTCACCCTTTACCAATCTAAT660.1263472251469265No Hit
TCGCCTGGGGGGGGCACAGGGCCCGGTGGAGCTGGGAATGTCCCGG630.12060416945842985No Hit
ATGCCTGGGACTTCATTGTCCCATGCTGGCTATCTTGAGCAAGCTA630.12060416945842985No Hit
ACAGCAGGGAGTTTTCCTTGCTTTGTCCACATTGCTGGCATCAGGC620.1186898175622643No Hit
AAGCTGGGGGCTCCTTCTGCCCAGTTTTCCTTGCTTTGTCCACATT620.1186898175622643No Hit
TATTATGGGGCAGTGGGATTGATCGCCATTGGTGTAGCGGTTCAGG620.1186898175622643No Hit
GACACTGGATGACCCACCAAACTCATGCATATCACATAGTTAATCC620.1186898175622643No Hit
AACAGCGGGGGTCGCTCTGCGCCGCGCAAGTCCCCAAATCCAGCTC610.11677546566609873No Hit
AACTACGGGCTGTTTATTGCTGCGTTTGTGGGTGCTGCGGCCACAC610.11677546566609873No Hit
CTAGCTGGGCAGGGACTCCATGCACATGCACCCCTGTATGCTTGTG600.11486111376993319No Hit
TCCAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA600.11486111376993319No Hit
GCATGCGGGGGGAATTCCCCGGGCCAATTGCCTCTATAACTAGAGC600.11486111376993319No Hit
GAACCGGGGGATGAGCCAGAAATCTTAATGAGAAGACCACTCGATC600.11486111376993319No Hit
AACTCTAGGGAGTGAGAGAGAAGCCTGCCAAGGGGGCCTGTCAGAA590.11294676187376765No Hit
ATATACGGGAGAGCTGGAAGGAGGATCAGCAAGTGAGGGGGCAGCC590.11294676187376765No Hit
CCAACTGGGGACATAAAACATCTTTCATGGTTTATTGCTATTCAGA580.11103240997760208No Hit
CGTCAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.11103240997760208No Hit
CTAAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.11103240997760208No Hit
AGTGCAGGGGGCTGAAGGTGAGCCGGACGCTATGGCGTTCTGCGCT580.11103240997760208No Hit
AGCAATGGGGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGT580.11103240997760208No Hit
CACTCCGGGATTCAGCAGAAGGTGATGGGGTTTGGGGAGCTCTGCC570.10911805808143653No Hit
ATAATAGGGGGGGTCTCTTAGCAACGGGCTTTGACAACACCTGAAC570.10911805808143653No Hit
AATAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA570.10911805808143653No Hit
TCTTACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA570.10911805808143653No Hit
AGTCACGGGGGGGTGGCATGCAGATCTTCGTGAAGACCCTGACTGG570.10911805808143653No Hit
CTACTTGGGGGCGCTGCGCAGGCGCAGAGTCTGGGTGGTGGATCAG570.10911805808143653No Hit
GCATCTCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATAC560.10720370618527096No Hit
TTCGTAGGGGTGGCATGTCCCCTGAGGAGTTGCACATGGTGTTTCC560.10720370618527096No Hit
TTAAAAGGGGCAGTTCGGCGCGCGCAAGTCGCCGGGAGCTGTAGTC560.10720370618527096No Hit
AATTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.10720370618527096No Hit
CGTAATGGGGGCGCTACTTCGGAGGAGCCCGACGCGGCGCGGTCGT560.10720370618527096No Hit
CGGACCGGGGTTCGGGTAAAAATGGCTGAATATTTAGCTTCGATAT560.10720370618527096No Hit
TTGGCGGGGAGGGAGTTATTCCACCTGCACCTTTCTGTACAAATAC560.10720370618527096No Hit
AACCTCGGGAGTATTCCAAAATCTATGGTATTGATTCAAAACAGAA560.10720370618527096No Hit
ACAAAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC560.10720370618527096No Hit
CACTATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC560.10720370618527096No Hit
CCTACTGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG550.10528935428910542No Hit
CCACAAGGAATTATTTTTTTAATCAGAAGGGTATATAACATTGGAC550.10528935428910542No Hit
ATCAACGGGAACTTACTATGGTTGGCATGCATAATAGCATTTCTTA550.10528935428910542No Hit
GCTATCGGGGGATCACTGGGAACAAACGGCCAAACTCAGGAAGCCT540.10337500239293988No Hit
ACGCCAGGGGGAAGGAGTTGACCAGCCAACCGACTCTGCATTCATC540.10337500239293988No Hit
ATCTGCGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT540.10337500239293988No Hit
ACCACTGGGTATCGCTGGGATCTGCAACTGGGGGGAATTCTGGACA540.10337500239293988No Hit
CGGGCTGGGGGAGCATGCGTGGGTCCAGCCACTAGGGCAGCTCTGC540.10337500239293988No Hit
TACAGGTGGGCTTACCGCGTGTCAGAAGGCTGGAAGCACTTGCTGG540.10337500239293988No Hit
CTCAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA540.10337500239293988No Hit
TTTCTAGGGAGACAAACCTTTGTCTTCTTCCCCCAGCTCTCCATCG540.10337500239293988No Hit
GTACCCGGGGGTGGAAAAATAACCATTCCCTGCCTCCTAGAAAGGA540.10337500239293988No Hit
CAAAGTGGGGTTGATTATCCAAGGAATGAAAGTGGAGACATGGCTG540.10337500239293988No Hit
TTCATTGGGGAGCGCGGCCGACTCTGCAGGTCTGCGTGGAGCCGCG530.10146065049677432No Hit
ATCAATGGGGGTGTAAATTTCCCTGGTGATGGTAACAATAGATAGA530.10146065049677432No Hit
AGCATCGGGACTGCCTTGTTGAATCTCTCTCTGTACTGTATGCACT530.10146065049677432No Hit
AATGCTGGGGTGCACATCATCTGCCCAGCACCAGCACTCAGGAGCT530.10146065049677432No Hit
AAATCAAGGGACCCAGAAAACCCCACAAAATCATGCAAATCAAGAG530.10146065049677432No Hit
ACACTAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC530.10146065049677432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)