FastQCFastQC Report
Sat 31 Dec 2022
SRR5843129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49944
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATTGGGAACCCCAGTAACCCCTACTGTGCAGGCATCCAGGGCA960.19221528111484865No Hit
TGCTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA690.13815473330129746No Hit
GAACCGGGGGATGAGCCAGAAATCTTAATGAGAAGACCACTCGATC690.13815473330129746No Hit
TCTAACGGGACCCATTTTTTTCCTTTGATTGTTTCTAACCTAACCA630.1261412782316194No Hit
ACACTAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC620.1241390357200064No Hit
TTAAAAGGGGCAGTTCGGCGCGCGCAAGTCGCCGGGAGCTGTAGTC610.12213679320839341No Hit
CGAAATGGGTGGCCAGGACACCTGAGCCCACATCTTTGGAGACCAA610.12213679320839341No Hit
GATATAGGGTCTGAGCAAATGGCTGCTCTTCGCATGCTGTGGATGG610.12213679320839341No Hit
TCTGGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA600.1201345506967804No Hit
AGTGCAGGGGGCTGAAGGTGAGCCGGACGCTATGGCGTTCTGCGCT600.1201345506967804No Hit
ATGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC590.11813230818516739No Hit
ACAAAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC590.11813230818516739No Hit
CTTACCGGGGTGGGTGGGTTCTGTCCTCTCTCCCCTAATGTCCCCA580.11613006567355438No Hit
AATTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.11613006567355438No Hit
CATTTAGGGGGCTCCTTCTGCCCAGTTTTCCTTGCTTTGTCCACAT570.11412782316194137No Hit
TGCTAAGGGGACTGCAGGAGTTCTCTGGTGGCCTCGTGCTGTCCCT570.11412782316194137No Hit
GTTCTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA570.11412782316194137No Hit
CGTAATGGGGGCGCTACTTCGGAGGAGCCCGACGCGGCGCGGTCGT570.11412782316194137No Hit
TACTAGGGGCATCCGAAAATTTGACAGGCAAGGACGGGGCCAGATC570.11412782316194137No Hit
CACACAGGGGAGACCCAGGGCTAAAGCTGAGAGATTGAAAAATGCA570.11412782316194137No Hit
TGCGCAGGGGTAAGGTGGCCGACGGAAAGGCCGTAACGGGCACCGA570.11412782316194137No Hit
ATATACGGGAGAGCTGGAAGGAGGATCAGCAAGTGAGGGGGCAGCC570.11412782316194137No Hit
GCCGCTGGGTCAGTGCCGCTGAGTAGTCAAAAGTGGAGACCGGATC560.11212558065032836No Hit
TATAATGGGTATGTGTGTGCATATATATGTGTGTGTCACGTTAATA560.11212558065032836No Hit
ATTGACGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTG560.11212558065032836No Hit
TAAGCAGGATGGGGTGAAGTAATTTTGAACGTACGCGATTTGTAGG550.11012333813871537No Hit
AACAATGGGGGAGGTGGGGGCCTCCGAAAAGATGGCGGCTGCTCCT550.11012333813871537No Hit
TGCTGCAGGGGGGTTTCTCTAGCTAAACAATAAATGTACCTGTCTC550.11012333813871537No Hit
TCAAGCGGGTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGA550.11012333813871537No Hit
ATCATGGGATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAG550.11012333813871537No Hit
GAACTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC550.11012333813871537No Hit
GGCATGGGCTGAAGAACCTTGTTCCCTTTGACTTCCCGAGTTGGAG540.10812109562710236No Hit
GCGCTTGGGGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGAC540.10812109562710236No Hit
AACAGCGGGGGTCGCTCTGCGCCGCGCAAGTCCCCAAATCCAGCTC540.10812109562710236No Hit
AGCTGAGGGGACGTGATCGAGGTTTGCGGTGAAGGAGGAAGTGTTT540.10812109562710236No Hit
AACTACGGGCTGTTTATTGCTGCGTTTGTGGGTGCTGCGGCCACAC540.10812109562710236No Hit
CGGGCTGGGGGAGCATGCGTGGGTCCAGCCACTAGGGCAGCTCTGC540.10812109562710236No Hit
TATCAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC540.10812109562710236No Hit
AATAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA540.10812109562710236No Hit
GCTACTGGGGAGACCATCCAAGAAGACCCCACAGCAGCTTCCGGAG540.10812109562710236No Hit
ACAATGGGGAATGGAAAGTTGCTCACTTTTCTGCTTTTCTATATGA540.10812109562710236No Hit
AATAAGGGATCCAAGTGATGGGGGAGGAGACACTCCCTTGGCACAG530.10611885311548935No Hit
ATCAACGGGAACTTACTATGGTTGGCATGCATAATAGCATTTCTTA530.10611885311548935No Hit
AGCAATGGGGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGT530.10611885311548935No Hit
GTTACAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC530.10611885311548935No Hit
ATATAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA530.10611885311548935No Hit
CCCGCAGGGGGGGAATTCATTTGATCGTAACTCGGGTGACCAATGA520.10411661060387634No Hit
AAGCTGGGGGCTCCTTCTGCCCAGTTTTCCTTGCTTTGTCCACATT520.10411661060387634No Hit
CATCCCGGCAAACAAGACCTGGCTTACCAGAGGCTGTGCCCAAAAG520.10411661060387634No Hit
GAACACGGGCCAACAAGTTGATGTTACTCAGAAACATCTGGAAGAG520.10411661060387634No Hit
TCTAACGGGATTCATTACCCCAACAATAATAGGATTCCCAATCGTT520.10411661060387634No Hit
CATTGCGGGATGACACTGCTTTTCTCCTTCGCCCAGGCTGTGGCCT520.10411661060387634No Hit
TTTGCTGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAAC520.10411661060387634No Hit
GGATGCGGGTGGCGAGTGAGGACTGCTTGTGGTATCTGGACCGGAA520.10411661060387634No Hit
ACCACTGGGTATCGCTGGGATCTGCAACTGGGGGGAATTCTGGACA520.10411661060387634No Hit
GGTTACGGGGAGGCCCGGAAGCTGCGGGCCGGCGTGAAGGAGGTGG520.10411661060387634No Hit
TCCAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA520.10411661060387634No Hit
TCGTGTGGGGATGGTGTAAAGAGAAGTCTTTCAAGAACTTTGGAAT520.10411661060387634No Hit
GTACCCGGGGGTGGAAAAATAACCATTCCCTGCCTCCTAGAAAGGA520.10411661060387634No Hit
CAAAGTGGGGTTGATTATCCAAGGAATGAAAGTGGAGACATGGCTG520.10411661060387634No Hit
ACACCTGGGTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC510.10211436809226333No Hit
TTCGTAGGGGTGGCATGTCCCCTGAGGAGTTGCACATGGTGTTTCC510.10211436809226333No Hit
CGCCTAGGGGGGAGTTGACCAGCCAACCGACTCTGCATTCATCTAG510.10211436809226333No Hit
CCAACTGGGGACATAAAACATCTTTCATGGTTTATTGCTATTCAGA510.10211436809226333No Hit
CCATCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA510.10211436809226333No Hit
TGCACTGGGTCTGGCTGAACTCTCTGGGAATTATTCCTCTGCTCCC510.10211436809226333No Hit
TCTCAATATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCC510.10211436809226333No Hit
TACAGGTGGGCTTACCGCGTGTCAGAAGGCTGGAAGCACTTGCTGG510.10211436809226333No Hit
CTCTAGGGGGGGAGCTTGCGGAGTAAGATGGCGGCCTCCAGGGCCT510.10211436809226333No Hit
ATCTGCGGGAAGGAGCTTGGCCTGAAACTTGAGTTAATGAAGAATT510.10211436809226333No Hit
AACCTCGGGAGTATTCCAAAATCTATGGTATTGATTCAAAACAGAA510.10211436809226333No Hit
TTCATAGGGACACGTCGGGTGCGAGGTTCCTGCGGACTAGCGGTGG510.10211436809226333No Hit
CTTCGCGGGATGCCTTTGACCTATTGGGCTTCGGAAGAAGTAGTAG500.10011212558065033No Hit
CATTGTGGGAATCTAGCCAGTGAGGGTACATTTAGGACTTGAACTT500.10011212558065033No Hit
ATAGAAGGGCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCC500.10011212558065033No Hit
ACGGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT500.10011212558065033No Hit
TTTGCCGGGGAGAGGCTCATGGTGCTGGCTCGAGCTAAGCCAAGCG500.10011212558065033No Hit
CAATATGGGGGGGCATAGACATCCCTCAGGCTTTGGTTGCTGGGGA500.10011212558065033No Hit
ATGCCTGGGACTTCATTGTCCCATGCTGGCTATCTTGAGCAAGCTA500.10011212558065033No Hit
GAAACAGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT500.10011212558065033No Hit
AAGTATGGGAGCGACCACAGCGGGAGAAGCAGCACTCTAGCCGCCT500.10011212558065033No Hit
GTATGCGGGTAGGAGTTGACCAGCCAACCGACTCTGCATTCATCTA500.10011212558065033No Hit
CCACCACACCCTGAGTGCAGGAACTGGGGAGGGTCCTGCTGGTGGG500.10011212558065033No Hit
CACGTAGGGGACTTTGGCGTGGCGTCACACCCGGAAGCAGCCCTGC500.10011212558065033No Hit
CTACTTGGGGGCGCTGCGCAGGCGCAGAGTCTGGGTGGTGGATCAG500.10011212558065033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)