FastQCFastQC Report
Sat 31 Dec 2022
SRR5843127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43367
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCGGGAAGTCTCGTCCTGACCTACTTGTCGTTTTTCTGTAAAG660.15218945280974014No Hit
TCGCATGGGGATAGAATGTGCGACCAATGTCTCCTGCACTTACGGG620.14296584960914982No Hit
GTAGGAGGGGTGATCCTGCATGCTTCAGTTTAGGGGAAGTATGGGA600.13835404800885465No Hit
CCTGCAGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT580.1337422464085595No Hit
TATCTAGGGATCCTGAAGGAGGAAAGATGACTCAGTACTTGGAGAA580.1337422464085595No Hit
AGCATAGGGGTGGGTTACCAGTATGGGAAGGCTGTCTTTGGAGGCT540.12451864320796921No Hit
TGAGTGGGGGTGATGAAAGATATCAGTATCTATACTTAGAACCCCA530.12221274240782161No Hit
AGTTATGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAG500.11529504000737888No Hit
GCTTCAGGGAGCCAAACCCATTCCCTCTTCCTCCCATGCAAGTTCT500.11529504000737888No Hit
TATCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA500.11529504000737888No Hit
ATAAATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC490.1129891392072313No Hit
CACATAGGGGCTGCACCATGAGGAGAAAGCAGAGACAGACAAGGAG490.1129891392072313No Hit
AGCTTTGGGTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTA480.11068323840708373No Hit
CAGCGGGTGCTGGCGTATATAGGGTTTGCATTACCAAGGGGCCTCT480.11068323840708373No Hit
TGTGCAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC480.11068323840708373No Hit
TTCTCAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC480.11068323840708373No Hit
GCAAGCGGGGCTGCATGGGAGACGCCTACGCACGGTCAAGGCCCAG480.11068323840708373No Hit
TGCAGTGGGGATGAGCTATAGTGCCAAATTTGCCTCCTGTTTCTAC480.11068323840708373No Hit
AACGCCGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCC470.10837733760693616No Hit
GCCTGCTGTCAGGGCTCGGGCTAATTTGGGCCTGAAGGAACAGTAG470.10837733760693616No Hit
TGAAACGGGATATGCAAGATCGGATTGATGACAATGGTTGGGGCTG470.10837733760693616No Hit
GTCCAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC470.10837733760693616No Hit
CAACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA470.10837733760693616No Hit
TAACACGGGGAGAAATCTGGTTTCAATAGAACCCACAGAAAAAGTT460.10607143680678857No Hit
AGCTAGGGGAACCTGGATTCCACGAAAAGAGCTGACCTCCCCTTCA460.10607143680678857No Hit
TACAGAGGGTATGCCACAACTAGATACATCAACATGATTTATCACA450.103765536006641No Hit
TGTAGCGGGACAGACATGCACTATCACATCTGGTAACCCTGACATT450.103765536006641No Hit
TACACCGGGATATCTCTCAGACCAGCAATCGTGGTTCCGGAAGCCT450.103765536006641No Hit
CAGTGAGGGGAGGCGACGGCGTGAGAGGCAGCGGCGGGATGGCCGG450.103765536006641No Hit
TTAGCAGGGGAATGGCAAATGGCTGCATTCTGCTCTGGCCATACCT450.103765536006641No Hit
TAAACTGGGGTACGAGCCGTGCCTGAACCCCGGTGTTCCTGAGAAT450.103765536006641No Hit
TCTCCCGGGTAGGATATTGTGAGTAATATAGATTGGCGCTAGTAGA440.10145963520649343No Hit
TAATATGGGATAAGCCAAGAGTAGTAGTTTCAGTGAACACACACAA440.10145963520649343No Hit
CACACTGGGACAGCCTCCTCTTGCAGATGTTTGTGGAGGAAAGAGG440.10145963520649343No Hit
TCACCAGGGAACTGGTTATTGGATCCTTTGAGAAGAGAAATATTTA440.10145963520649343No Hit
CCATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA440.10145963520649343No Hit
TTGCGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA440.10145963520649343No Hit
CCTAGTGGGGGCTATCAAGCTGAGGAAGACGCGGAAAGTGAAGGTT440.10145963520649343No Hit
AGTTCGGGGAATCAGTCATGACCAAGAGAGGCCGGCCATTCCTCAG440.10145963520649343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)