FastQCFastQC Report
Tue 3 Jan 2023
SRR5843043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254514
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAG304.852569E-640.0000041
GGGTCTA304.852569E-640.0000047
TCGATTT304.852569E-640.00000414
ACGGGTA304.852569E-640.0000045
CACAACG304.852569E-640.0000041
TATACGG304.852569E-640.0000042
TCAACGG304.852569E-640.0000042
CTCGTAC200.001253927940.029
TTTACTC200.001253927940.026
TTACTCT200.001253927940.027
GATCACG200.001253927940.01
CAACACG200.001253927940.01
GCCGAAA200.001253927940.031
AGTTAAT900.040.039
ATAACAG200.001253927940.01
CGTTTTA200.001253927940.012
CTCTATG200.001253927940.01
AATACGG200.001253927940.01
TACGTGA200.001253927940.021
TCAAACG200.001253927940.01