FastQCFastQC Report
Sat 31 Dec 2022
SRR5843011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59064
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTGGGCGATCTGCTGTCGGGGATAAAATCTAGTAAGAAACTG1200.2031694433157253No Hit
ACGCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAA870.14729784640390087No Hit
GAGATAGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT840.14221861032100772No Hit
TTTAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAA800.13544629554381687No Hit
AACGCTGGGAAGGTTCGAGGACTTATTGAGATCATTTCCAATGCAG770.13036705946092375No Hit
CCGGCGGGGCAGCTTTATTGCTCTCTTCCCCCAAAGAATGTCGTTT740.1252878233780306No Hit
TACCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAA730.1235947446837329No Hit
GCTACTGGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCA710.12020858729513748No Hit
AGACCTGGGATCTTCGGCCGGGGAGACCTGCCCCTGAGGAAGTGAC690.11682242990654204No Hit
TTTGCTGGGGAGTTCTTGAATGACTTTGGACACCCAGATGTTTCAG690.11682242990654204No Hit
CTCGCTGGGAGCCCCAAGGAATTGGCTATCCACCGCAGCAGCAGCA680.11512935121224435No Hit
TTCACGCGGGGGCTGCTCAGACCCTGGAGGCTAAGGACTTGTTTCG670.11343627251794662No Hit
CGCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGT660.11174319382364893No Hit
TTGCTTGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCA660.11174319382364893No Hit
AGTATAGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT650.1100501151293512No Hit
CACTAGGGGGTGAGCTGAATGTTACTGAGGATGCTCTGAAAGCCAT650.1100501151293512No Hit
AGTTACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCT640.10835703643505351No Hit
CGTGCTGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAG630.10666395774075578No Hit
TAGCTGGGGGACTCTGCCATGGAGAGCTACTTCCAGGCTGGAGCGC630.10666395774075578No Hit
AGCTTGGGGGCAACCAACCCTGGGAGCAGGCCCTGAACCGCTTCTG630.10666395774075578No Hit
TTATCAAGGGGGAGTCTGTTCCGCTGTAGAGGTGACCTGACTGCTG630.10666395774075578No Hit
TGGATAGGGGCGGCGGGGCGCAACGGTGTCTCCTTCACTTCGCCCT620.10497087904645808No Hit
TGACTCGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAG620.10497087904645808No Hit
TTAAAAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC620.10497087904645808No Hit
CACAATGGGGCATTCCCACGAATAAATAATATAAGTTTTTGACTCC620.10497087904645808No Hit
AGCATAGGGGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCT610.10327780035216036No Hit
GTATCCGGGGGGGTCACCTGAACAGAAAGGTGCTGAACAAGCTGAA610.10327780035216036No Hit
TAATCAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC610.10327780035216036No Hit
GGCAATGGGATTCCGAAGAAGAGGCCAACTCCGAAAAATTCCCCTT610.10327780035216036No Hit
TTTGTAGGGGAAGGCAAAGGGGATTGGATTGAGGAAGGAACTGGTG610.10327780035216036No Hit
TGGCCTAGGATTGCCTTTTGTTTTTAAATCAGGAATTGAGACAAGG610.10327780035216036No Hit
AACACAGGGGGGAATGATTGCTGGTGGGACAGGAATCACCCCAATG600.10158472165786266No Hit
GCCTGTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC600.10158472165786266No Hit
ATTGAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTC600.10158472165786266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)