FastQCFastQC Report
Sat 31 Dec 2022
SRR5842999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5842999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39734
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTACGGGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTC540.13590376000402676No Hit
AATGATGGGGGGGAAAGGGGCTGGCCCGGAAGAAGGAGAAAGCCTG490.12332007852217244No Hit
AGTCTAGGGGGCCTGAGGCTTCCTGGGGGCACACAAAGAAAGCCAT460.11576986963305984No Hit
CTTGTCGGGACCGGTTTGGCGGTCCTCTCGGCTCTGCCCTACCCCG460.11576986963305984No Hit
TCATCAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC450.11325313333668899No Hit
CGTAATGGGAGGGTCGCGAGGCGGCGTATGGCGGACGCGGTGCTAT450.11325313333668899No Hit
CATTAAGGGGGGGTGGGAAGGACCTTGGCCTCTTTCTGTGTTTATA450.11325313333668899No Hit
AACGTCGGGCAACGGCATGATCCCTATGACCGCGTCATGCTAACTG450.11325313333668899No Hit
TTTAGCGGGGGACTTGGTTCTGTATGTTTGGGGAGGTGTGTGTGTG440.11073639704031811No Hit
TTTTAGGGGGGAGGAGGACCCGCAGCGCGCCTCCTCGCAGCCACTG440.11073639704031811No Hit
CTTGAGGAGGGGGTGAGATGTCATGGCTTCCTTCTAGGCTGCGGGA430.10821966074394726No Hit
CCAATTGGGGCTCCTCCGTGGCCGTTACCAGAAGAGGCCGTTGAGT430.10821966074394726No Hit
TCTTACGGGGCTTTCCTCTGGCGGCAGCCATCAGGTAGGGCGGCAG420.10570292444757638No Hit
TTCTAAGGGCTCTTGCTTCTTGCTCTCTTTTCCTGAAGATGTAAGA420.10570292444757638No Hit
GCTATAGGGTAACAGAGTCTGTGCTAGTGAACTTCTTTGAGAAGAG400.10066945185483464No Hit
CTTTTAGGGGATGCAGCGAAGCCCTTCAGCGTGGTGGTCTTCCACT400.10066945185483464No Hit
AACAGTGGGGAGGCCATGGCTTACTTTGCCATGGTCAAGAGGTACC400.10066945185483464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)