FastQCFastQC Report
Sat 31 Dec 2022
SRR5842977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5842977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54883
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA1150.209536650693293No Hit
AGGCTTGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCA960.17491755188309677No Hit
GTTTCTGGGACTGAAGAATGGGCTGAGGACTTAGCTCAGTTGGTAG780.14212051090501612No Hit
AAGTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA740.13483227957655375No Hit
ACTTTAGGGAAGCCAATGATTTGGCAAGCCTTCTGTTAGTATTGGA740.13483227957655375No Hit
TAAACAGGGGTTCCGGGAGAAGGTCAATGAACAGCACCGGCAGATG700.1275440482480914No Hit
CATTTGGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTA700.1275440482480914No Hit
GACATAGGGAAGTGAAGACGCTGACTGGGAAGGAGATTGAGATAGA700.1275440482480914No Hit
TAGTTAGGGGACTGAGACACTGTGGTTTAATCTGGACCGACCCTGT690.12572199041597582No Hit
CATCTAGGGCGATCTGTCTGCGGGTCACTCGCCTGTGGAAGGCGAT690.12572199041597582No Hit
TCTTTAGGGAAGGAAAACAATAAAGAGAAGTTATTTGATTTGGTAG680.12389993258386021No Hit
GCCTCTGGGATGGAGTATATAGTATATATGAAAATGAGGAACTTAC680.12389993258386021No Hit
GCCATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA680.12389993258386021No Hit
TTATTAGGGCGATCTTCAAGTGGGATGGCTCAGATGGCATCCACCG680.12389993258386021No Hit
CTCATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA670.12207787475174463No Hit
TCCAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA660.12025581691962903No Hit
TCGTCTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC660.12025581691962903No Hit
CGTATCGGGAAGTCTGTGTGGTTGTTATTTGAATAAAGTTGTGTAA660.12025581691962903No Hit
ATATCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA660.12025581691962903No Hit
AACCAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA650.11843375908751344No Hit
ATTAAAGGGATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGT650.11843375908751344No Hit
TCTACAGGGATTCCTCGGTGAGGACATACACTTGTTACATTTAGCC650.11843375908751344No Hit
ATAACTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC650.11843375908751344No Hit
CTTGATGGGGAAGGCTGTGTCTGGTACCCGCTACCATGCCCAAGCG650.11843375908751344No Hit
TACTCCGGGGGTTACAGTGCATTGGGTTTTCTACATTCTCAGTTTG650.11843375908751344No Hit
CAACTAGGGGTTCCAGAAGTGGACACTGAGCACCCAGCCTTCCTGT640.11661170125539784No Hit
CTACAGGGATTTCAGAGTCAGAGCTGTAACAGGTAAACCAAACTGT640.11661170125539784No Hit
ATTACAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT640.11661170125539784No Hit
TAAACTGGGCGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTC640.11661170125539784No Hit
CAACTAGGGGTGGTTCTGATGGCTTCCAGTTTTATTCTGGAGTCTC630.11478964342328225No Hit
CGAAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA630.11478964342328225No Hit
AAAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA630.11478964342328225No Hit
AATTCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA630.11478964342328225No Hit
GGCTCTGGGGTTTCAAATGGTTCAGTATACATGAATATAGAAATAT630.11478964342328225No Hit
AGAACTGGGGTTCCCAGATGGTTTATTTTATTTTTCTACATTTTAT630.11478964342328225No Hit
AATTCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC630.11478964342328225No Hit
TGCTCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA620.11296758559116665No Hit
CCGTAGGGAGACCAAGATCAGCTGCTTCCACCTCAGCAACAAACCT620.11296758559116665No Hit
GCTTAAGGGGCTTCCAAAAAACGGGTGGGAATGATTTTTGAGTGAC620.11296758559116665No Hit
TATGCAGGGATGACCCACCAAACTCATGCATATCACATAGTTAATC620.11296758559116665No Hit
TCTCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA620.11296758559116665No Hit
GACACAGGGAATCAAACCTTAGGCTTGTGTATGCTAGGCAAGCACT610.11114552775905108No Hit
GGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATG610.11114552775905108No Hit
ACGTGGGAAGACAAGTAGGTGTTCACTTAACTTCTGTCTATGCCTA610.11114552775905108No Hit
GTTCGGGGTCCTGTGGGCCTCCACCACAGCTACCCCTGGCTCAAAT610.11114552775905108No Hit
CTTGCAGGGATGGGAGAAGGCACTCTCCCACTCTCTGATGTGTGCA610.11114552775905108No Hit
CTCACGGGACTACTGAGCTCAGCGGCAAAGGAAGAACGAGGGGCAG610.11114552775905108No Hit
CAAGCTGGGGAACTGTAGAATATTATTTGAAAGTGATTCAGAGTAG610.11114552775905108No Hit
ATCTGAGGGATTGATCAAAGTGCCAGTGAACCCCAGACCAGACTGA610.11114552775905108No Hit
TTCACGGGGGATCTCGACAGGGGGGGAATTCTGGACATTAATTAGG610.11114552775905108No Hit
CTTGCTGGGATCCGACTTGAGAAAACCACCCTGTTTGTGACAACTG610.11114552775905108No Hit
GCGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA600.1093234699269355No Hit
GCGCTAGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC600.1093234699269355No Hit
CTAGTTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACT600.1093234699269355No Hit
GCCAATGGGGTTCAGGTGCCAGGGATGGTGAAGAGTCTAAGACTCA600.1093234699269355No Hit
ACTTCAGGGTTACTGACAGGTTTTAACTTCATATTGCAAATAAGGC600.1093234699269355No Hit
TTGACCGGGAAAGATGGAAATGGAAGACTAGACCCACTTCCTCTGA600.1093234699269355No Hit
TGCCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA600.1093234699269355No Hit
GCTGCAGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACAT590.10750141209481989No Hit
CTGTAAGGGGTGGCACAGCCTCTGTACTTTGTCAGCAAGATGGAGA590.10750141209481989No Hit
GTTCCAGGGCCACTTTGATTTGCTTTCTAGATTTCCTATGTCTCAG590.10750141209481989No Hit
ATACTTGGGTGTTCTGATTCTTTGGGCACCCAGAAGTTTATATTCT590.10750141209481989No Hit
GTCAAGGGGATGTGTAAACCAATCTTTTCCAAGATTGTATCCCTTT590.10750141209481989No Hit
TCTGGCTTAGAGACTCAGGGAAGGGGCTGAGGATGGTATGTGGTAA590.10750141209481989No Hit
CTCAAAGGGGGCAGATGAGTGCATCCAAATAATGGGGGGCATGGGC580.1056793542627043No Hit
TGCAAGGGGAGACCATAAAGAGTGACTGGCAGATGTCAGAGCTGCT580.1056793542627043No Hit
ACGCAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.1056793542627043No Hit
CCACTCGGGGGTAGAGAACTTGCCTTAAATGTACCTAGCAAGCAAT580.1056793542627043No Hit
TTCACCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.1056793542627043No Hit
TTATCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.1056793542627043No Hit
TTGGCTAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCT580.1056793542627043No Hit
AATGCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA580.1056793542627043No Hit
AGTATAGGGATACAACCGAAGGTCCTTCAGAGACCAGCTCCCCCTG580.1056793542627043No Hit
TAATTAGGGGACGGTGGTTAGAGCGATGCTGAACAACCTGGTGAGT580.1056793542627043No Hit
TAACTTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC570.1038572964305887No Hit
GATACTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC570.1038572964305887No Hit
TACCTCGGGACTTCGTGAGTTCTAAGGAGCTACAGAGGAGACCCTG570.1038572964305887No Hit
ACCAGGGTGGGATCGAGCGGCTGCGAGGCGGTGCAGTGTGCCAATG570.1038572964305887No Hit
AGCATACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA570.1038572964305887No Hit
CTTAGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA570.1038572964305887No Hit
TACAGGGAGTGCCAAACAGGTCAGATTTAATGACAGTTACCGAATC570.1038572964305887No Hit
TCTACTGGGATCCCTGAATTCAATGATACTCTGAATTTGCTGGTCA570.1038572964305887No Hit
TGCTAAGGGAAGGTACATGGGTAAGAGAATCTAACATCTTATACTT570.1038572964305887No Hit
TGGGCAGGGGGAGCCACCTTGAACCCTGATCCTGACTGCAGCCGCA560.10203523859847312No Hit
TGAACTGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCT560.10203523859847312No Hit
TCGCCCGGGACCCACAGTGCTATTGTTTATGACAGCCAAAAGGTAG560.10203523859847312No Hit
ACCCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.10203523859847312No Hit
GCTATAGGGACTTCGGCTCAGTCTCCTGAACTCTTTCAGTCTTTAA560.10203523859847312No Hit
TCTCACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.10203523859847312No Hit
TATTCAGGGGGTCACTGTGAGAAAATTAAGAAGGCTTGTGGAAATT560.10203523859847312No Hit
ACAAGGGGCCACTTCTCTGCTTTGGTTGCCATGGAGAACGATGGCT560.10203523859847312No Hit
TTCCAAGGGAGCAGTTATTGTCACACAGCACTGCGGTATCCAGAGA560.10203523859847312No Hit
ACACAAGGGGGCTGTATGAAAATTGGCACTTCCTTTTCTGTTGAAG560.10203523859847312No Hit
ACTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA560.10203523859847312No Hit
AAGTCAGGGAATCCCATGCCCACATGATAGCCTACAGGTATAGACT550.10021318076635752No Hit
ACGCAAGGGGTCTGTCGGGGTGGGCAGTACAGGATTCCTCCCTCCT550.10021318076635752No Hit
GTCATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA550.10021318076635752No Hit
TCTCAGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCC550.10021318076635752No Hit
TACTGCGGGACAACTGCAAGTCCTTTCCTGAGCCCATAAGAAACAG550.10021318076635752No Hit
ATAACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG550.10021318076635752No Hit
GCAACTGGGGATTGGAAGTCATTCCTCGGTTGCACTAGCCACACAA550.10021318076635752No Hit
TTGGTTGGGACGGCAAGGCAACACCAAGAATGTTCTGCTGGCATGT550.10021318076635752No Hit
ATCAAGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAA550.10021318076635752No Hit
TCGCCTGGGGGGAACAGGAAGTGGACCACCTTAAAGCCCACCCCCA550.10021318076635752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)