FastQCFastQC Report
Thu 19 Jan 2023
SRR5842815.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5842815.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79887
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG304.810776E-640.0000042
GTTGGGA304.810776E-640.0000044
CGGGTAT200.001248765540.06
TTAATTG200.001248765540.01
CGACGTT257.729605E-540.027
TTGCTTA200.001248765540.034
GACGTTT257.729605E-540.028
TATGGCC451.1914381E-940.010
GCTGCGC200.001248765540.037
TTCAATG200.001248765540.01
GTTAGGG200.001248765540.03
GTCTAAA257.729605E-540.010
TGCGGGT200.001248765540.04
TAGTTAA353.0089905E-740.038
CTTATGG451.1914381E-940.02
TTCTTGG353.0089905E-740.02
GCTTATG257.729605E-540.01
TCTAACA200.001248765540.013
GCTTAAT200.001248765540.036
TAGGGGT555.456968E-1240.05