FastQCFastQC Report
Thu 19 Jan 2023
SRR5842481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5842481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249641
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC200.001253881540.021
ACTATCG200.001253881540.01
AAATCCG200.001253881540.01
CAACGTC200.001253881540.020
TCGGGCG257.777108E-540.05
GCCGGAT200.001253881540.04
TATTCCG200.001253881540.01
CGTACAG257.777108E-540.01
TACGTGG257.777108E-540.02
AATCGCG200.001253881540.01
GCCGATC353.0435694E-739.9999969
GTCCGAT353.0435694E-739.9999969
TATGGGC950.035.7894744
GGCCGAT455.4133125E-835.5555538
TTATCGG455.4133125E-835.5555532
CGGGCGC455.4133125E-835.5555536
ATCGCGG455.4133125E-835.5555532
CGCGGGC408.663701E-735.04
TAAACGG408.663701E-735.02
CGCGCGG408.663701E-735.02
Produced by FastQC (version 0.11.5)