FastQCFastQC Report
Thu 19 Jan 2023
SRR5842311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5842311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75029
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGA304.8068523E-640.0000045
TAAAACG304.8068523E-640.00000421
AAAACGA304.8068523E-640.00000422
CAGATAA304.8068523E-640.00000417
TCAGATA304.8068523E-640.00000416
AACGAAT304.8068523E-640.00000424
CTCCTGG304.8068523E-640.0000042
ACGAATA304.8068523E-640.00000425
TCTGCAC200.001248279840.013
TTCTAGG200.001248279840.02
CGGGTGT200.001248279840.06
TTGTGGG200.001248279840.03
CAAGTAT200.001248279840.034
TTCAGAT401.8873834E-840.015
CGACGTT200.001248279840.027
GGCACCG200.001248279840.08
TTTCGCG200.001248279840.01
TATGGGC257.725098E-540.04
CGCATGG200.001248279840.02
TGGGTAC200.001248279840.06