FastQCFastQC Report
Thu 19 Jan 2023
SRR5842121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5842121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75085
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATTA200.001248285440.027
ATGGGTT200.001248285440.05
CGGGTAT257.725149E-540.06
TTAGGGA1100.040.04
TCGTTGA200.001248285440.024
CATATGG401.8873834E-840.02
TGGGTCT304.806898E-640.06
TTGTAGG257.725149E-540.02
CAATTGG257.725149E-540.02
AATGGGC257.725149E-540.04
ATATGGG750.040.03
GTAGGGC353.0057527E-740.04
CAGGGTT257.725149E-540.05
CTAACGG200.001248285440.02
ACAGGGT555.456968E-1240.04
TGATTAT200.001248285440.028
TGTTCAT200.001248285440.014
TAGGGCG200.001248285440.05
CTACCTT257.725149E-540.017
GGGCCTA304.806898E-640.07
Produced by FastQC (version 0.11.5)