FastQCFastQC Report
Thu 19 Jan 2023
SRR5841667.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5841667.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77216
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA257.727211E-540.05
ATGGGCT401.8884748E-840.05
CGGAAGA200.001248507940.022
GGGTGAT200.001248507940.08
TCGCAGG257.727211E-540.02
ACCGGGC401.8884748E-840.04
CTCCGGT200.001248507940.015
TAAAGGG304.808695E-640.03
ATAATGG401.8884748E-840.02
TGGGTGC200.001248507940.06
CGGTTAT200.001248507940.018
GGACTGT200.001248507940.09
GGACTGC200.001248507940.08
GACAAAC200.001248507940.025
GGCCGAT304.808695E-640.08
TTCACGG257.727211E-540.02
ATATGTA200.001248507940.012
AGGGTAA200.001248507940.06
GGATAGG257.727211E-540.08
TGCGGGC257.727211E-540.04