FastQCFastQC Report
Thu 19 Jan 2023
SRR5841635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5841635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121339
Sequences flagged as poor quality0
Sequence length46
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG304.8315524E-640.0000042
TCAACGG304.8315524E-640.0000042
CCACAAT200.001251334240.040
GATACGG200.001251334240.02
CGAACTG257.753446E-540.01
AAATCGG200.001251334240.02
TTACTCA200.001251334240.029
TACGGGC257.753446E-540.04
TGCGGGC257.753446E-540.04
GTAATCT200.001251334240.028
AACGGGC401.9039362E-840.04
TTAAGCG200.001251334240.01
AATTCAC200.001251334240.013
CACACCG200.001251334240.017
CTAATGG401.9039362E-840.02
TTATGGG1050.038.095243
ATGGGGT1800.036.6666685
AACATAC601.2732926E-1136.66666816
TACGAAA601.2732926E-1136.66666820
TGGGATC601.2732926E-1136.6666686
Produced by FastQC (version 0.11.5)