FastQCFastQC Report
Thu 19 Jan 2023
SRR5841605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5841605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15583
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAACTGTCTCTTATACACATCTGACGCTTGGTACAATCTCGTAT470.3016107296412758No Hit
AAAGCTGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGC170.10909324263620612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTGT200.001217962840.0000043
CGGGGAA200.001217962840.0000046
ATAGGGA200.001217962840.0000044
AGCAGAG200.001217962840.00000438
AATAGGG200.001217962840.0000043
CTGTGGG200.001217962840.0000043
AAAACTG200.001217962840.0000042
GGGAAGA200.001217962840.0000048
ACAGGGC200.001217962840.0000044
CAGTGGT200.001217962840.00000428
ATCTGGG200.001217962840.0000043
CTCGTAT200.001217962840.00000440
TATGGGA401.7271304E-840.0000044
ACTGTCT257.445342E-540.05
ATGGGAG451.0641088E-940.05
AAACTCA257.445342E-540.020
TGCATAT257.445342E-540.027
CACATAG257.445342E-540.034
AACTGTC257.445342E-540.04
TGACCCA257.445342E-540.011