FastQCFastQC Report
Thu 19 Jan 2023
SRR5841590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5841590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54171
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATGGGCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCT640.11814439460227798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCAC200.001245203540.0000047
ACGGGAA401.8706487E-840.0000045
GGATTAA200.001245203540.0000048
TATCGCG200.001245203540.0000041
CAATAGG200.001245203540.0000042
ATCGCGG200.001245203540.0000042
GTAACAG200.001245203540.0000041
TTGGGGT200.001245203540.0000045
GGATCTC257.69658E-540.08
TCACTGG304.7820376E-640.02
AAGGGAC700.040.05
GGATTTC304.7820376E-640.08
TGGGAGT304.7820376E-640.06
TTTCTCG257.69658E-540.01
TTCTCGG257.69658E-540.02
ATGGGAG900.037.7777755
AATAGGG750.037.3333323
CACGGGA700.037.1428574
ATAGGGA1350.037.0370374
TAGGGAG601.2732926E-1136.6666645