FastQCFastQC Report
Thu 19 Jan 2023
SRR5840582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5840582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164850
Sequences flagged as poor quality0
Sequence length46
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATAGA304.8421516E-640.00000436
AATCACG200.00125264340.01
GCTATTG200.00125264340.01
CTGAATA200.00125264340.028
GCCGATC200.00125264340.09
TAACCAA200.00125264340.014
TACGAAA353.035184E-740.020
AAACCGG200.00125264340.02
CGGGCGA750.040.06
CCGAGAC200.00125264340.020
CGGGATA507.6397555E-1140.06
ACGCTTG257.7656E-540.01
CATAATC200.00125264340.018
ATGCGCA200.00125264340.018
AGCCTAG200.00125264340.01
GCGTGAG200.00125264340.01
ATAGTGG200.00125264340.02
TGGGACG200.00125264340.06
AATACAG200.00125264340.01
ATATTGG200.00125264340.02
Produced by FastQC (version 0.11.5)