FastQCFastQC Report
Thu 19 Jan 2023
SRR5840211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5840211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392083
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG200.001254756940.0000041
TCGAATG200.001254756940.0000041
ATAATCG257.785251E-540.01
ATTACCG257.785251E-540.01
CATTACG257.785251E-540.01
GTCCGAT304.859301E-640.09
ACGGGTA601.4551915E-1136.6666645
AACGAAT503.4124241E-936.024
ACGAATA503.4124241E-936.025
TAGCTAG455.426955E-835.5555531
TATCCGG455.426955E-835.5555532
CTTAAGG1750.035.428572
ATACTCG408.68049E-735.0000041
TTCTTCG408.68049E-735.0000041
TAGTAGG800.035.0000042
TACGGGC2000.035.04
TTGTACG351.3987206E-534.2857131
ATATACG351.3987206E-534.2857131
TAGGGCG701.8189894E-1234.2857135
TCAAACG351.3987206E-534.2857131
Produced by FastQC (version 0.11.5)