FastQCFastQC Report
Fri 10 Feb 2017
ERR1633526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430028
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26980.627401006446092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.4395062647083446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9920.23068265322258086No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9360.21766024537937062No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9090.21138158445496574No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8500.19766154762015498No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7200.16743095798413127No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7070.1644078990205289No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6750.15696652311012307No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6730.1565014371157227No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6310.14673463123331504No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6290.14626954523891467No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6000.1395257983201094No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5730.13324713739570446No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5670.13185187941250337No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5340.12417796050489735No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5340.12417796050489735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.12138744453849518No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5220.12138744453849518No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5150.11975964355809389No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5150.11975964355809389No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4980.11580641260569079No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4940.11487624061689006No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4870.1132484396364888No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4730.10999283767568623No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4700.10929520868408568No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4610.10720232170928405No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4450.10348163375408113No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4440.10324909075688095No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4380.10185383277367985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAA250.005492564829.615
TACGCTA250.005492564829.69
GGTATCA3400.028.2941191
TGCGGTA659.363248E-825.61538536
GTATCAA11500.025.5782591
GTGACAC2300.023.32608824
TTAACGG2250.023.02222335
CCTCGGA502.698292E-422.237
AGTGACA2550.021.76470623
AACGGCC2350.021.2553237
TTGCGGT705.089678E-621.14285735
GCATCAG1850.021.015
CCAGGAC2300.020.9130443
TAACGGC2500.020.7236
TTTAACG2600.020.63461534
ACATGTT2600.020.63461529
GCGACCT450.003821972520.55555733
TAGCACT450.003821972520.5555574
GCACCGC2700.020.55555310
ACCTCGG555.1359023E-420.18181836
Produced by FastQC (version 0.11.5)