##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633526.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430028
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.863769335950217	33.0	33.0	33.0	33.0	33.0
2	31.97794097128559	33.0	33.0	33.0	33.0	33.0
3	31.694019924284	33.0	33.0	33.0	27.0	33.0
4	31.971908805938217	33.0	33.0	33.0	33.0	33.0
5	32.08244346879738	33.0	33.0	33.0	33.0	33.0
6	35.25471597198322	37.0	37.0	37.0	33.0	37.0
7	35.39493009757504	37.0	37.0	37.0	33.0	37.0
8	35.524228654878286	37.0	37.0	37.0	33.0	37.0
9	35.6278405127108	37.0	37.0	37.0	33.0	37.0
10	35.630421739979724	37.0	37.0	37.0	33.0	37.0
11	35.65082041169412	37.0	37.0	37.0	33.0	37.0
12	35.650185569311766	37.0	37.0	37.0	33.0	37.0
13	35.645874222143675	37.0	37.0	37.0	33.0	37.0
14	35.62014333950347	37.0	37.0	37.0	33.0	37.0
15	35.6317193299041	37.0	37.0	37.0	33.0	37.0
16	35.623666365911056	37.0	37.0	37.0	33.0	37.0
17	35.630731022165996	37.0	37.0	37.0	33.0	37.0
18	35.614383249462826	37.0	37.0	37.0	33.0	37.0
19	35.61028119099221	37.0	37.0	37.0	33.0	37.0
20	35.606774442594435	37.0	37.0	37.0	33.0	37.0
21	35.604900146037004	37.0	37.0	37.0	33.0	37.0
22	35.510087715218546	37.0	37.0	37.0	33.0	37.0
23	35.55795204033226	37.0	37.0	37.0	33.0	37.0
24	35.550410670933054	37.0	37.0	37.0	33.0	37.0
25	35.57616945873292	37.0	37.0	37.0	33.0	37.0
26	35.459728203744874	37.0	37.0	37.0	33.0	37.0
27	35.47952923995647	37.0	37.0	37.0	33.0	37.0
28	35.5015696652311	37.0	37.0	37.0	33.0	37.0
29	35.50503223045941	37.0	37.0	37.0	33.0	37.0
30	35.50059298464286	37.0	37.0	37.0	33.0	37.0
31	35.4810547220181	37.0	37.0	37.0	33.0	37.0
32	35.484824244002716	37.0	37.0	37.0	33.0	37.0
33	35.480782646711376	37.0	37.0	37.0	33.0	37.0
34	35.46656031700261	37.0	37.0	37.0	33.0	37.0
35	35.38535165152037	37.0	37.0	37.0	33.0	37.0
36	35.419630814737644	37.0	37.0	37.0	33.0	37.0
37	35.40701303170956	37.0	37.0	37.0	33.0	37.0
38	35.38175421135368	37.0	37.0	37.0	33.0	37.0
39	35.29044155264308	37.0	37.0	37.0	33.0	37.0
40	35.074653278391175	37.0	37.0	37.0	33.0	37.0
41	35.2455398253137	37.0	37.0	37.0	33.0	37.0
42	35.272882230924495	37.0	37.0	37.0	33.0	37.0
43	34.94085036323216	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	18.0
16	22.0
17	10.0
18	21.0
19	18.0
20	23.0
21	65.0
22	159.0
23	443.0
24	904.0
25	1616.0
26	2708.0
27	4189.0
28	5903.0
29	8146.0
30	10450.0
31	13378.0
32	17196.0
33	22700.0
34	33063.0
35	66966.0
36	242028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93152073818449	21.07211623429172	13.549815360860224	24.446547666663566
2	16.118485307933437	21.518831331913272	39.04931771884622	23.313365641307076
3	17.14376738258904	28.147469467104468	29.91456370282865	24.794199447477837
4	11.944571051187365	17.91929827825165	40.859664952049634	29.276465718511353
5	13.158678039569516	37.90799668858772	35.76022956644684	13.173095705395927
6	28.533025756462372	41.092440492247015	17.33631298427079	13.038220767019823
7	26.139228143283695	32.57508813379594	24.052154743412057	17.23352897950831
8	24.079362274084477	35.93161375538337	20.61237872882696	19.37664524170519
9	25.837154789920657	15.08948254532263	20.556568409498915	38.5167942552578
10	15.13971183271787	27.800282772284596	34.06196805789391	22.99803733710363
11	33.315272493884116	24.29678997646665	24.22098095937939	18.166956570269843
12	23.133609904471335	26.461067651408744	29.547610853246763	20.85771159087315
13	26.96289543936674	22.752239389063035	26.50199521891598	23.782869952654245
14	21.66696122112979	21.208851516645428	28.400476248058265	28.72371101416652
15	23.9768108123192	28.68627159161729	25.522059028714413	21.814858567349102
16	22.416447301105975	28.5683722920368	25.508338991879597	23.50684141497763
17	21.972522719450826	27.787260364441384	26.332006287962646	23.90821062814514
18	22.09065456202852	27.504022993851564	28.62046192340964	21.78486052071028
19	24.4925911801092	26.280149199587004	27.872836187411053	21.35442343289274
20	23.461262987526393	26.430139432781118	28.41931223083148	21.689285348861002
21	22.65410624424456	27.28729292046099	28.367920228450238	21.690680606844207
22	22.107397657826933	27.264271163738172	28.027709823546378	22.60062135488852
23	22.245993284158242	27.113118215558057	28.801147832234182	21.839740668049522
24	22.683871747886183	28.016082673686366	26.48408940813156	22.815956170295888
25	22.540857804608073	27.231250058135746	28.422800375789482	21.805091761466695
26	22.709451477578206	27.549368878305597	28.327922832931808	21.41325681118439
27	22.78991135460947	27.332638804915028	27.194043178583723	22.683406661891784
28	21.70905150362302	27.02824002157999	28.881375166268242	22.38133330852875
29	23.986810161198804	26.79662719636861	27.452165905475923	21.764396736956662
30	23.225231845368207	26.368980624517473	28.52023589161636	21.885551638497958
31	22.403424893262763	26.830578473959836	28.35420019161543	22.41179644116197
32	21.180481270987006	26.765466434743786	28.656506087975668	23.397546206293544
33	21.54324834661929	26.969174100291145	29.009971443719945	22.47760610936962
34	22.499465151106442	26.839880193847844	28.75394160380254	21.906713051243177
35	22.72945017533742	27.379147404355063	27.897253202117074	21.994149218190444
36	23.519863822820838	27.214274419340136	27.15404578306529	22.111815974773734
37	22.266922153906258	26.534551238524003	27.925855990772696	23.272670616797043
38	22.926181550968774	27.020798645669586	27.490302957016755	22.562716846344888
39	21.78672086468788	27.311012306175414	28.230952403099334	22.671314426037377
40	22.295059856567477	27.78074916051978	28.237463607020942	21.686727375891802
41	21.92647920600519	26.348749383761056	28.460704884333115	23.26406652590064
42	21.660682560205384	27.87213855841945	27.939110941613105	22.52806793976206
43	22.862464769735922	25.935520477736336	28.099100523686833	23.102914228840913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	55.5
2	69.0
3	173.5
4	278.0
5	278.0
6	375.5
7	473.0
8	406.5
9	340.0
10	474.0
11	608.0
12	608.0
13	1010.0
14	1412.0
15	2439.5
16	3467.0
17	3958.0
18	4449.0
19	4449.0
20	3958.0
21	3467.0
22	3600.0
23	3733.0
24	4657.5
25	5582.0
26	5582.0
27	7170.5
28	8759.0
29	10193.0
30	11627.0
31	13975.0
32	16323.0
33	16323.0
34	20578.5
35	24834.0
36	26745.5
37	28657.0
38	30967.5
39	33278.0
40	33278.0
41	34467.0
42	35656.0
43	36332.5
44	37009.0
45	35443.5
46	33878.0
47	33878.0
48	31906.0
49	29934.0
50	29992.0
51	30050.0
52	28306.0
53	26562.0
54	26562.0
55	26225.0
56	25888.0
57	21795.5
58	17703.0
59	15869.0
60	14035.0
61	14035.0
62	12590.5
63	11146.0
64	8803.0
65	6460.0
66	5294.5
67	4129.0
68	4129.0
69	3440.5
70	2752.0
71	2419.0
72	2086.0
73	2234.5
74	2383.0
75	2383.0
76	2053.0
77	1723.0
78	1252.0
79	781.0
80	484.5
81	188.0
82	188.0
83	146.5
84	105.0
85	94.5
86	84.0
87	64.0
88	44.0
89	44.0
90	34.5
91	25.0
92	16.0
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	430028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.67255086681217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48138376786493	42.957509342415754
2	6.9296474726459625	6.884265331481661
3	1.9653318820243668	2.9286914364006917
4	0.9228094312125019	1.8335319364910805
5	0.5946970830060557	1.4770060552981563
6	0.42581262015180166	1.2690719420532566
7	0.3307328793542521	1.149984204113594
8	0.24684395941117038	0.9809095304013344
9	0.2177897785965755	0.9736356470049142
>10	1.5261264581795762	15.200726837777642
>50	0.20538304344658154	7.129257917505523
>100	0.14343456166454385	13.14601169435935
>500	0.009054008875834995	2.983404721014535
>1k	9.530535658773678E-4	1.085993403682536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2698	0.627401006446092	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1890	0.4395062647083446	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	992	0.23068265322258086	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	936	0.21766024537937062	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	909	0.21138158445496574	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	850	0.19766154762015498	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	720	0.16743095798413127	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	707	0.1644078990205289	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	675	0.15696652311012307	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	673	0.1565014371157227	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	631	0.14673463123331504	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	629	0.14626954523891467	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	600	0.1395257983201094	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	573	0.13324713739570446	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	567	0.13185187941250337	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	534	0.12417796050489735	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	534	0.12417796050489735	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.12138744453849518	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	522	0.12138744453849518	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	515	0.11975964355809389	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	515	0.11975964355809389	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	498	0.11580641260569079	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	494	0.11487624061689006	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	487	0.1132484396364888	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	473	0.10999283767568623	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	470	0.10929520868408568	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	461	0.10720232170928405	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	445	0.10348163375408113	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	444	0.10324909075688095	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	438	0.10185383277367985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	2.3254299720018232E-4	0.0	0.0
5	0.0	0.0	4.6508599440036463E-4	0.0	0.0
6	0.0	0.0	0.0011627149860009116	0.0	0.0
7	0.0	0.0	0.002092886974801641	2.3254299720018232E-4	0.0
8	0.0	0.0	0.002790515966402188	2.3254299720018232E-4	0.0
9	0.0	0.0	0.0034881449580027347	9.301719888007293E-4	0.0
10	2.3254299720018232E-4	0.0	0.003953230952403099	0.0011627149860009116	0.0
11	2.3254299720018232E-4	0.0	0.006511203921605105	0.0018603439776014585	0.0
12	2.3254299720018232E-4	0.0	0.0069762899160054695	0.0018603439776014585	0.0
13	2.3254299720018232E-4	0.0	0.007441375910405834	0.0018603439776014585	0.0
14	2.3254299720018232E-4	0.0	0.0076739189076060164	0.002092886974801641	0.0
15	2.3254299720018232E-4	0.0	0.007906461904806199	0.002092886974801641	0.0
16	2.3254299720018232E-4	0.0	0.008836633893606928	0.002325429972001823	0.0
17	2.3254299720018232E-4	0.0	0.009301719888007293	0.0025579729692020055	0.0
18	2.3254299720018232E-4	0.0	0.009534262885207475	0.002790515966402188	0.0
19	2.3254299720018232E-4	0.0	0.00999934887960784	0.002790515966402188	0.0
20	2.3254299720018232E-4	0.0	0.00999934887960784	0.002790515966402188	0.0
21	2.3254299720018232E-4	0.0	0.00999934887960784	0.0032556019608025524	0.0
22	2.3254299720018232E-4	0.0	0.010231891876808022	0.0032556019608025524	0.0
23	2.3254299720018232E-4	0.0	0.010696977871208387	0.0032556019608025524	0.0
24	2.3254299720018232E-4	0.0	0.010696977871208387	0.004185773949603282	0.0
25	2.3254299720018232E-4	0.0	0.010696977871208387	0.004418316946803464	0.0
26	2.3254299720018232E-4	0.0	0.010696977871208387	0.005581031932804376	0.0
27	2.3254299720018232E-4	0.0	0.010696977871208387	0.007441375910405834	0.0
28	2.3254299720018232E-4	0.0	0.010696977871208387	0.024417014706019143	0.0
29	2.3254299720018232E-4	0.0	0.010696977871208387	0.048136400420437736	0.0
30	2.3254299720018232E-4	0.0	0.010696977871208387	0.07952970504246235	0.0
31	2.3254299720018232E-4	0.0	0.010696977871208387	0.14557191624731414	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCAA	25	0.0054925648	29.6	15
TACGCTA	25	0.0054925648	29.6	9
GGTATCA	340	0.0	28.294119	1
TGCGGTA	65	9.363248E-8	25.615385	36
GTATCAA	1150	0.0	25.578259	1
GTGACAC	230	0.0	23.326088	24
TTAACGG	225	0.0	23.022223	35
CCTCGGA	50	2.698292E-4	22.2	37
AGTGACA	255	0.0	21.764706	23
AACGGCC	235	0.0	21.25532	37
TTGCGGT	70	5.089678E-6	21.142857	35
GCATCAG	185	0.0	21.0	15
CCAGGAC	230	0.0	20.913044	3
TAACGGC	250	0.0	20.72	36
TTTAACG	260	0.0	20.634615	34
ACATGTT	260	0.0	20.634615	29
GCGACCT	45	0.0038219725	20.555557	33
TAGCACT	45	0.0038219725	20.555557	4
GCACCGC	270	0.0	20.555553	10
ACCTCGG	55	5.1359023E-4	20.181818	36
>>END_MODULE