FastQCFastQC Report
Fri 10 Feb 2017
ERR1633525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484477
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62691.2939726756894547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.987043760591318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21710.4481120878803328No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19660.40579841767514246No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14450.29825977290975625No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12880.26585369377700074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.25553328640988116No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11860.2448000627480768No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11300.23324120649690283No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9610.19835822959603863No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8570.17689178227242985No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8490.17524051709369073No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7980.16471370157922874No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.157489416422245No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7440.15356766162273958No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7290.1504715394126037No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7280.1502651312652613No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7150.1475818253498102No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6830.14097676463485367No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6800.1403575401928265No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6790.1401511320454841No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6140.12673460246822862No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5830.12033594990061447No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5810.1199231336059297No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5710.11785905213250578No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5450.11249244030160359No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5430.11207962400691879No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5380.11104758327020683No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5350.11042835882817967No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5340.11022195068083729No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5260.10857068550209814No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5220.10774505291272858No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5120.10568097143930466No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4980.10279125737651115No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4980.10279125737651115No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4980.10279125737651115No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4970.10258484922916877No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4890.10093358405042965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACC200.001840885437.01
CGGTTGT303.5964325E-430.83333221
ACGGTTG250.005493244629.59999820
TGCGGCA358.8619604E-426.4285723
GGTATCA8500.025.2470591
GTATCAA25950.024.6666681
ACACGAA701.9181425E-723.78571317
ACCCCGC551.899714E-523.5454546
TCAATAC400.001929583723.1253
TTCGCCG2000.023.12499824
GCCGCTC2050.022.56097627
GGACGTA753.7315294E-722.227
GTATAGA502.698977E-422.1999991
CCGGCAG2250.021.37777716
GCCGGCA2350.021.2553215
GCTCTCT2100.021.14285730
CGCTCTC2100.021.14285729
CCGCTCT2200.021.02272828
CTGCGGC450.00382266220.55555522
GCTCGTC450.00382266220.55555521
Produced by FastQC (version 0.11.5)