##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	484477
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.902125384693186	33.0	33.0	33.0	33.0	33.0
2	31.993217428278328	33.0	33.0	33.0	33.0	33.0
3	31.708968640410173	33.0	33.0	33.0	27.0	33.0
4	31.987371949545594	33.0	33.0	33.0	33.0	33.0
5	32.089965055100656	33.0	33.0	33.0	33.0	33.0
6	35.224357399835284	37.0	37.0	37.0	33.0	37.0
7	35.39884865535412	37.0	37.0	37.0	33.0	37.0
8	35.54312588626498	37.0	37.0	37.0	33.0	37.0
9	35.66083013228698	37.0	37.0	37.0	33.0	37.0
10	35.63709113126113	37.0	37.0	37.0	33.0	37.0
11	35.653446912856545	37.0	37.0	37.0	33.0	37.0
12	35.66479316871595	37.0	37.0	37.0	33.0	37.0
13	35.65939559566295	37.0	37.0	37.0	33.0	37.0
14	35.63746266592635	37.0	37.0	37.0	33.0	37.0
15	35.659329545055805	37.0	37.0	37.0	33.0	37.0
16	35.65525504822726	37.0	37.0	37.0	33.0	37.0
17	35.64820620999552	37.0	37.0	37.0	33.0	37.0
18	35.63055212115333	37.0	37.0	37.0	33.0	37.0
19	35.60979571785658	37.0	37.0	37.0	33.0	37.0
20	35.61443164484589	37.0	37.0	37.0	33.0	37.0
21	35.59414791620655	37.0	37.0	37.0	33.0	37.0
22	35.49166627105105	37.0	37.0	37.0	33.0	37.0
23	35.529558678740166	37.0	37.0	37.0	33.0	37.0
24	35.55040590162175	37.0	37.0	37.0	33.0	37.0
25	35.56453041114439	37.0	37.0	37.0	33.0	37.0
26	35.44611405701406	37.0	37.0	37.0	33.0	37.0
27	35.437789616431736	37.0	37.0	37.0	33.0	37.0
28	35.44047292234719	37.0	37.0	37.0	33.0	37.0
29	35.45932417844397	37.0	37.0	37.0	33.0	37.0
30	35.459580124546676	37.0	37.0	37.0	33.0	37.0
31	35.44820084338369	37.0	37.0	37.0	33.0	37.0
32	35.429489944827104	37.0	37.0	37.0	33.0	37.0
33	35.40726391552128	37.0	37.0	37.0	33.0	37.0
34	35.34661707366913	37.0	37.0	37.0	33.0	37.0
35	35.27644655989861	37.0	37.0	37.0	33.0	37.0
36	35.27480561512724	37.0	37.0	37.0	33.0	37.0
37	35.254088429378484	37.0	37.0	37.0	33.0	37.0
38	35.20964875525567	37.0	37.0	37.0	33.0	37.0
39	35.08881329763848	37.0	37.0	37.0	33.0	37.0
40	34.84012656947595	37.0	37.0	37.0	27.0	37.0
41	34.985437905204996	37.0	37.0	37.0	27.0	37.0
42	34.968822049343935	37.0	37.0	37.0	27.0	37.0
43	34.58739836978845	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	14.0
16	23.0
17	17.0
18	14.0
19	28.0
20	33.0
21	89.0
22	241.0
23	536.0
24	1011.0
25	1893.0
26	3068.0
27	4789.0
28	6860.0
29	9444.0
30	12530.0
31	16080.0
32	20407.0
33	26787.0
34	38146.0
35	74909.0
36	267557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.260670785197235	19.466558784008324	13.900763090920726	23.37200733987372
2	16.581798516751054	22.572588585216636	38.26641925210072	22.579193645931593
3	19.16417084815172	27.489849879354438	29.763642030478227	23.582337242015615
4	12.50172866823399	18.180429617917877	39.19009571145792	30.127746002390204
5	14.695228050041592	36.22896442968397	33.079588917533755	15.996218602740687
6	27.972638535988292	40.012012954175326	17.33931641749763	14.676032092338748
7	26.24190622052234	32.67379049985861	21.862131742064122	19.22217153755493
8	25.424323548899125	32.7350937196193	20.65980428379469	21.180778447686887
9	27.53236995770697	13.887759377638154	19.534054248189285	39.04581641646559
10	16.541342519871943	26.745542100037774	31.48694365263986	25.226171727450424
11	34.72404262741059	23.80959261223959	22.886948193619098	18.57941656673072
12	24.15904160569026	25.357860125454874	29.27445472127676	21.20864354757811
13	30.295968642474257	22.40292108810119	24.942979749296665	22.35813052012789
14	23.36953044210561	21.110599677590475	28.78815712613808	26.73171275416583
15	25.68976442638144	26.83161429747955	25.340109024783423	22.138512251355586
16	21.813213011143976	27.505330490405118	25.39191746976637	25.289539028684537
17	22.63946482495557	25.776249440117898	26.023526400634083	25.56075933429244
18	24.240779232037845	23.864703587580006	28.711373295326716	23.183143885055433
19	26.043135174631615	24.70788086947368	28.39866495210299	20.850319003791718
20	26.095769252203922	23.477481903165682	28.331169487922025	22.095579356708367
21	24.113218996980248	25.31286314933423	28.424259562373443	22.149658291312075
22	23.119363767526632	25.501520196005178	28.463064294073813	22.916051742394377
23	23.14330711261835	25.232363971870697	29.306035167820145	22.31829374769081
24	23.03618128414765	25.226171727450424	28.36460760779149	23.37303938061043
25	23.457253904726127	25.75437017649961	29.113249958202346	21.675125960571915
26	23.89917374818619	25.470558973903817	28.142306033103736	22.487961244806254
27	23.856447261686313	25.43381832367687	28.751416475911135	21.958317938725678
28	22.719551186124416	25.79627103041011	28.257688187468137	23.226489595997332
29	23.722901190355785	25.517413623350542	27.587480933047388	23.172204253246285
30	23.660153113563698	25.237317767406914	28.950600338096542	22.151928780932842
31	24.069873286038344	24.85050889928727	27.8558115245925	23.223806290081882
32	21.820024480006275	25.351255064739913	28.99043711053363	23.838283344720182
33	22.224997265092046	25.405953223785648	29.32543753367033	23.043611977451974
34	21.779568483127164	25.721138464777482	28.561727388503478	23.937565663591872
35	22.509840508424546	26.06006064271369	28.39866495210299	23.031433896758774
36	23.29274661129424	25.50853807301482	28.556154368525235	22.64256094716571
37	23.990612557458867	25.49656640046896	27.761276593109685	22.75154444896249
38	23.279123673569643	25.659216020574764	27.454966902453577	23.60669340340202
39	22.944948883022313	25.132668836704326	28.437676091950703	23.484706188322665
40	22.756704652646047	26.46152448929464	28.131779217589276	22.64999164047003
41	23.232888248564947	24.996439459458344	28.338393773079012	23.432278518897697
42	22.14408527133383	26.240254955343595	28.42797490902561	23.187684864296966
43	22.723472940923926	25.887916247830134	27.83083613876407	23.55777467248187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25.0
1	59.0
2	93.0
3	379.0
4	665.0
5	665.0
6	795.0
7	925.0
8	930.0
9	935.0
10	1296.5
11	1658.0
12	1658.0
13	2570.5
14	3483.0
15	6018.0
16	8553.0
17	9030.5
18	9508.0
19	9508.0
20	7655.5
21	5803.0
22	4948.0
23	4093.0
24	4616.5
25	5140.0
26	5140.0
27	5940.0
28	6740.0
29	7702.0
30	8664.0
31	9967.5
32	11271.0
33	11271.0
34	13901.5
35	16532.0
36	17292.5
37	18053.0
38	19935.0
39	21817.0
40	21817.0
41	24694.5
42	27572.0
43	30441.5
44	33311.0
45	35888.0
46	38465.0
47	38465.0
48	41269.0
49	44073.0
50	44045.0
51	44017.0
52	43997.5
53	43978.0
54	43978.0
55	40484.0
56	36990.0
57	32985.0
58	28980.0
59	25504.0
60	22028.0
61	22028.0
62	18754.5
63	15481.0
64	12290.0
65	9099.0
66	7887.0
67	6675.0
68	6675.0
69	5521.0
70	4367.0
71	3464.0
72	2561.0
73	2006.5
74	1452.0
75	1452.0
76	1050.5
77	649.0
78	484.5
79	320.0
80	259.0
81	198.0
82	198.0
83	163.0
84	128.0
85	105.0
86	82.0
87	74.5
88	67.0
89	67.0
90	44.5
91	22.0
92	12.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	484477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.67669790373287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.61787140508065	38.19388251523052
2	8.337791657603276	7.61685581457218
3	2.608133581225987	3.57392789046721
4	1.3069998456585306	2.387977484414807
5	0.768976432876102	1.7562152109785913
6	0.5222717905244884	1.4313390479657229
7	0.3781942073232129	1.209226378979088
8	0.31582039807810014	1.154050633188003
9	0.23317935816195215	0.9585776790134835
>10	1.5513362826263164	14.094705242460742
>50	0.18662454043690996	5.8883558447587925
>100	0.15759405636888524	13.89139252924367
>500	0.011059232025886684	3.3234607802657252
>1k	0.0036864106752955614	3.200541141527588
>5k	4.608013344119452E-4	1.3194918069338712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6269	1.2939726756894547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4782	0.987043760591318	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2171	0.4481120878803328	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1966	0.40579841767514246	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1445	0.29825977290975625	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1288	0.26585369377700074	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1238	0.25553328640988116	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1186	0.2448000627480768	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1130	0.23324120649690283	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	961	0.19835822959603863	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	857	0.17689178227242985	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	849	0.17524051709369073	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	798	0.16471370157922874	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	763	0.157489416422245	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	744	0.15356766162273958	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	729	0.1504715394126037	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	728	0.1502651312652613	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	715	0.1475818253498102	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	683	0.14097676463485367	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	680	0.1403575401928265	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	679	0.1401511320454841	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	614	0.12673460246822862	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	583	0.12033594990061447	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	581	0.1199231336059297	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	571	0.11785905213250578	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	545	0.11249244030160359	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	543	0.11207962400691879	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	538	0.11104758327020683	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	535	0.11042835882817967	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	534	0.11022195068083729	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	526	0.10857068550209814	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	522	0.10774505291272858	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	512	0.10568097143930466	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	498	0.10279125737651115	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	498	0.10279125737651115	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	498	0.10279125737651115	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	497	0.10258484922916877	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	489	0.10093358405042965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.256325893695676E-4	0.0	0.0
2	0.0	0.0	8.256325893695676E-4	0.0	0.0
3	0.0	0.0	0.0012384488840543515	0.0	0.0
4	0.0	0.0	0.002270489620766311	0.0	0.0
5	0.0	0.0	0.0028897140627934864	0.0	0.0
6	0.0	0.0	0.00660506071495654	0.0	0.0
7	0.0	0.0	0.013829345871940258	0.0	0.0
8	2.064081473423919E-4	0.0	0.015067794755994608	0.0	0.0
9	4.128162946847838E-4	0.0	0.019402365850184837	4.128162946847838E-4	0.0
10	0.0010320407367119596	0.0	0.021466447323608757	6.192244420271757E-4	0.0
11	0.0012384488840543515	0.0	0.03034199765933161	8.256325893695676E-4	0.0
12	0.0012384488840543515	0.0	0.03405734431149467	8.256325893695676E-4	0.0
13	0.0012384488840543515	0.0	0.03550220134289141	0.0010320407367119596	0.0
14	0.0012384488840543515	0.0	0.03859832355302729	0.0010320407367119596	0.0
15	0.0012384488840543515	0.0	0.0406624050264512	0.0012384488840543515	0.0
16	0.0014448570313967432	0.0	0.04561620056266861	0.0014448570313967432	0.0
17	0.0014448570313967432	0.0	0.04664824129938057	0.001651265178739135	0.0
18	0.001651265178739135	0.0	0.04809309833077731	0.001651265178739135	0.0
19	0.001651265178739135	0.0	0.04891873092014688	0.001651265178739135	0.0
20	0.001651265178739135	0.0	0.049950771656858836	0.001857673326081527	0.0
21	0.001857673326081527	0.0	0.05284048571965232	0.001857673326081527	0.0
22	0.001857673326081527	0.0	0.05407893460370668	0.002270489620766311	0.0
23	0.001857673326081527	0.0	0.054491750898391464	0.004128162946847838	0.0
24	0.001857673326081527	0.0	0.054904567193076244	0.005366611830902189	0.0
25	0.001857673326081527	0.0	0.05511097534041864	0.006192244420271757	0.0
26	0.001857673326081527	0.0	0.05511097534041864	0.008049917746353285	0.0
27	0.001857673326081527	0.0	0.05511097534041864	0.01197167254585873	0.0
28	0.001857673326081527	0.0	0.05511097534041864	0.03508938504820662	0.0
29	0.001857673326081527	0.0	0.05511097534041864	0.08875550335722851	0.0
30	0.001857673326081527	0.0	0.05511097534041864	0.15996631419035373	0.0
31	0.001857673326081527	0.0	0.05511097534041864	0.27658691743880515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACC	20	0.0018408854	37.0	1
CGGTTGT	30	3.5964325E-4	30.833332	21
ACGGTTG	25	0.0054932446	29.599998	20
TGCGGCA	35	8.8619604E-4	26.42857	23
GGTATCA	850	0.0	25.247059	1
GTATCAA	2595	0.0	24.666668	1
ACACGAA	70	1.9181425E-7	23.785713	17
ACCCCGC	55	1.899714E-5	23.545454	6
TCAATAC	40	0.0019295837	23.125	3
TTCGCCG	200	0.0	23.124998	24
GCCGCTC	205	0.0	22.560976	27
GGACGTA	75	3.7315294E-7	22.2	27
GTATAGA	50	2.698977E-4	22.199999	1
CCGGCAG	225	0.0	21.377777	16
GCCGGCA	235	0.0	21.25532	15
GCTCTCT	210	0.0	21.142857	30
CGCTCTC	210	0.0	21.142857	29
CCGCTCT	220	0.0	21.022728	28
CTGCGGC	45	0.003822662	20.555555	22
GCTCGTC	45	0.003822662	20.555555	21
>>END_MODULE