FastQCFastQC Report
Fri 10 Feb 2017
ERR1633524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431561
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27470.6365264701861382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.46714137746459944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10490.24307108380970474No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8190.18977618459499354No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8010.1856052794390596No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7640.17703175217408432No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6080.14088390748932364No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6040.13995703967689388No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6010.13926188881757157No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5710.13231038022434835No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.12999321069327396No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5490.12721260725598468No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5410.12535887163112516No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5100.1181756460847945No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5090.11794392913168705No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5020.11632191045993498No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4990.11562675960061265No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4560.10566293061699272No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4410.10218717632038113No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4390.10172374241416623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTC702.055458E-1029.07142827
CCGCTCT652.9795046E-928.46153828
GGTATCA4450.026.191011
GCTTCGC856.91216E-1126.11764722
CGCTCTC751.3686076E-824.66666629
GCCGGCA851.9681465E-923.94117715
GTATCAA13150.023.4942971
TTCGCCG952.8012437E-1023.36842224
AGCTTCG1005.311449E-1022.221
CGCCGCT957.1140676E-921.42105326
CGGCAGC1059.786163E-1021.14285917
TGCTCGC1059.786163E-1021.14285910
CACGGTA450.003821995320.5555552
AGTTACG450.003821995320.55555530
ACGGTAC450.003821995320.5555553
TCGCCGC1001.2842065E-820.3525
ACGGGTA555.1359425E-420.18181817
TTCTGCG555.1359425E-420.18181818
AGGTCGC1101.7462298E-920.18181835
TATTAGA2150.019.7906972
Produced by FastQC (version 0.11.5)