##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633524.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431561
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.851675661146395	33.0	33.0	33.0	27.0	33.0
2	31.959013905334356	33.0	33.0	33.0	33.0	33.0
3	31.683463056207582	33.0	33.0	33.0	27.0	33.0
4	31.97808652774463	33.0	33.0	33.0	33.0	33.0
5	32.07252045481403	33.0	33.0	33.0	33.0	33.0
6	35.226190503775825	37.0	37.0	37.0	33.0	37.0
7	35.369709033021984	37.0	37.0	37.0	33.0	37.0
8	35.479232368077746	37.0	37.0	37.0	33.0	37.0
9	35.606305945161864	37.0	37.0	37.0	33.0	37.0
10	35.5788822437616	37.0	37.0	37.0	33.0	37.0
11	35.611932032783315	37.0	37.0	37.0	33.0	37.0
12	35.60907959709056	37.0	37.0	37.0	33.0	37.0
13	35.59615442544623	37.0	37.0	37.0	33.0	37.0
14	35.58746735687423	37.0	37.0	37.0	33.0	37.0
15	35.58799798869685	37.0	37.0	37.0	33.0	37.0
16	35.58568545350484	37.0	37.0	37.0	33.0	37.0
17	35.58092367011848	37.0	37.0	37.0	33.0	37.0
18	35.57666239535083	37.0	37.0	37.0	33.0	37.0
19	35.566585025060185	37.0	37.0	37.0	33.0	37.0
20	35.55228577188393	37.0	37.0	37.0	33.0	37.0
21	35.56183946186055	37.0	37.0	37.0	33.0	37.0
22	35.461348453636916	37.0	37.0	37.0	33.0	37.0
23	35.51134138626984	37.0	37.0	37.0	33.0	37.0
24	35.48838055338643	37.0	37.0	37.0	33.0	37.0
25	35.515957187975744	37.0	37.0	37.0	33.0	37.0
26	35.41508616394901	37.0	37.0	37.0	33.0	37.0
27	35.4210806815259	37.0	37.0	37.0	33.0	37.0
28	35.42972372387681	37.0	37.0	37.0	33.0	37.0
29	35.43655705682395	37.0	37.0	37.0	33.0	37.0
30	35.4324301778891	37.0	37.0	37.0	33.0	37.0
31	35.414296009138916	37.0	37.0	37.0	33.0	37.0
32	35.40430900845998	37.0	37.0	37.0	33.0	37.0
33	35.404216321678746	37.0	37.0	37.0	33.0	37.0
34	35.39200020391092	37.0	37.0	37.0	33.0	37.0
35	35.32392176308795	37.0	37.0	37.0	33.0	37.0
36	35.35597748638084	37.0	37.0	37.0	33.0	37.0
37	35.351220337333544	37.0	37.0	37.0	33.0	37.0
38	35.321581421861566	37.0	37.0	37.0	33.0	37.0
39	35.232388932271455	37.0	37.0	37.0	33.0	37.0
40	35.01117107430931	37.0	37.0	37.0	27.0	37.0
41	35.18030359555196	37.0	37.0	37.0	33.0	37.0
42	35.2149360113634	37.0	37.0	37.0	33.0	37.0
43	34.87693049186558	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	12.0
16	18.0
17	13.0
18	15.0
19	11.0
20	28.0
21	93.0
22	224.0
23	531.0
24	961.0
25	1846.0
26	2961.0
27	4540.0
28	6269.0
29	8478.0
30	10834.0
31	13905.0
32	17312.0
33	23081.0
34	33100.0
35	67216.0
36	240109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.917043013618006	21.193527682065803	13.642335614200544	24.24709369011565
2	16.100852486670483	21.5452740168829	39.05079467329068	23.30307882315594
3	17.117163042999714	28.188830779426315	30.101654227328233	24.592351950245735
4	11.912105125347287	18.15502327596794	40.72031532042979	29.212556278254986
5	13.032688310574866	37.84424449846024	35.890638866811415	13.232428324153481
6	28.28452988105969	41.11655131024351	17.4904590544558	13.108459754241
7	25.94998157850223	32.70522591244343	24.06612274973874	17.2786697593156
8	23.987802419588427	35.912188543450405	20.68606755476051	19.413941482200663
9	25.726143001800438	15.164252562210208	20.61956478921867	38.490039646770676
10	15.057431046827679	28.002298632174828	33.98824268179933	22.952027639198167
11	33.099376449679184	24.40002687916656	24.350902885107782	18.14969378604647
12	22.934185433808892	26.444928990339722	29.86576636906486	20.755119206786528
13	27.00985492201566	22.8813539685004	26.54734788361321	23.561443225870736
14	21.826114964049115	21.247981166046053	28.363545362069324	28.562358507835512
15	23.84784537991153	28.779477292897184	25.456424468383382	21.91625285880791
16	22.309244811278127	28.732670468369477	25.661262254930357	23.296822465422036
17	21.723464353822518	27.964528768818315	26.474820477290578	23.83718640006859
18	22.069186047858818	27.53654755642887	28.71876745118303	21.675498944529277
19	24.49943345204965	26.32930223073911	28.161256462006527	21.01000785520471
20	23.59342016539956	26.581410275720003	28.391583113395324	21.433586445485112
21	22.47098324454712	27.35418631433332	28.438853371829243	21.73597706929032
22	22.205667333239102	27.207741199969416	28.100314903339275	22.48627656345221
23	22.067795746140177	27.165568714503856	28.94770380085318	21.818931738502783
24	22.54698640516636	27.74972715328772	26.948218212489078	22.755068229056842
25	22.340990033853846	27.251767421059824	28.85084611445427	21.556396430632056
26	22.52265612509008	27.626685451187665	28.54521145330556	21.305446970416696
27	22.52659531329291	27.350710560036706	27.623904847750374	22.498789278920015
28	21.781857026005593	27.092577874275015	28.929398161557696	22.196166938161696
29	23.67405766508095	26.896777048899228	27.679748633449268	21.749416652570552
30	23.047263306925323	26.459990592291703	28.52945470049425	21.96329140028872
31	22.434371965956146	26.972085058659147	28.437694787063705	22.155848188321002
32	21.33302128783648	26.67502392477541	28.79917323391131	23.192781553476795
33	21.44100138798455	26.878934843509956	29.271412384344274	22.408651384161217
34	22.39590695174031	26.818688435702022	28.821649778362733	21.963754834194933
35	22.443177210174227	27.564353590801765	27.908684983119418	22.08378421590459
36	23.293114994172317	27.126408549428703	27.505960918618687	22.07451553778029
37	22.26452343932839	26.66830413313529	28.02732406311043	23.039848364425886
38	22.555791649384442	27.247828232857003	27.725165156258324	22.471214961500227
39	21.784637629442884	27.279573455432722	28.393205132067077	22.54258378305732
40	22.164421715585977	27.77359399945778	28.363777079022434	21.69820720593381
41	21.97974330395935	26.59299612337537	28.422633185111724	23.004627387553555
42	21.444940576187378	27.87415915710641	28.294261993090203	22.386638273616015
43	22.610940284224014	26.154819365049203	28.296579162621278	22.937661188105505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	51.5
2	67.0
3	207.0
4	347.0
5	347.0
6	381.5
7	416.0
8	373.5
9	331.0
10	499.0
11	667.0
12	667.0
13	1087.0
14	1507.0
15	2593.5
16	3680.0
17	4144.5
18	4609.0
19	4609.0
20	4216.0
21	3823.0
22	3931.5
23	4040.0
24	5193.5
25	6347.0
26	6347.0
27	7806.5
28	9266.0
29	10765.5
30	12265.0
31	14524.0
32	16783.0
33	16783.0
34	20433.5
35	24084.0
36	26236.0
37	28388.0
38	30366.5
39	32345.0
40	32345.0
41	33742.0
42	35139.0
43	35945.5
44	36752.0
45	35556.0
46	34360.0
47	34360.0
48	32565.5
49	30771.0
50	30689.0
51	30607.0
52	28688.0
53	26769.0
54	26769.0
55	26324.5
56	25880.0
57	21775.5
58	17671.0
59	15892.5
60	14114.0
61	14114.0
62	12524.5
63	10935.0
64	8703.5
65	6472.0
66	5324.0
67	4176.0
68	4176.0
69	3446.5
70	2717.0
71	2351.0
72	1985.0
73	1955.5
74	1926.0
75	1926.0
76	1593.5
77	1261.0
78	914.5
79	568.0
80	375.0
81	182.0
82	182.0
83	147.5
84	113.0
85	102.5
86	92.0
87	69.0
88	46.0
89	46.0
90	34.5
91	23.0
92	12.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	431561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.11318319240858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48565561710043	45.07042814689515
2	6.973075238990536	7.267782946879242
3	1.955253085436461	3.056833866866172
4	1.0021285127044506	2.0889642705961196
5	0.599897700748213	1.5631289387898164
6	0.4256833848329001	1.3310229729456888
7	0.3195545347607756	1.1657102806967237
8	0.24333715714046047	1.0144859078064585
9	0.17913376453455365	0.840170761842148
>10	1.5058172313091962	15.491788832890832
>50	0.187817005194544	6.914626985727336
>100	0.11540562969781512	10.95165782393655
>500	0.0058834242591043	1.87264268594931
>1k	0.0013577132905625306	1.3707555781785126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2747	0.6365264701861382	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2016	0.46714137746459944	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.24307108380970474	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	819	0.18977618459499354	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	801	0.1856052794390596	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	764	0.17703175217408432	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	608	0.14088390748932364	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	604	0.13995703967689388	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	601	0.13926188881757157	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	571	0.13231038022434835	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	561	0.12999321069327396	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	549	0.12721260725598468	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	541	0.12535887163112516	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	510	0.1181756460847945	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	509	0.11794392913168705	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	502	0.11632191045993498	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	499	0.11562675960061265	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	456	0.10566293061699272	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	441	0.10218717632038113	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	439	0.10172374241416623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.317169531074402E-4	0.0	0.0
2	0.0	0.0	2.317169531074402E-4	0.0	0.0
3	0.0	0.0	2.317169531074402E-4	0.0	0.0
4	0.0	0.0	2.317169531074402E-4	0.0	0.0
5	0.0	0.0	4.634339062148804E-4	0.0	0.0
6	0.0	0.0	0.001158584765537201	0.0	0.0
7	0.0	0.0	0.0027806034372892825	0.0	0.0
8	2.317169531074402E-4	0.0	0.003707471249719043	0.0	0.0
9	2.317169531074402E-4	0.0	0.005097772968363685	0.001158584765537201	0.0
10	2.317169531074402E-4	0.0	0.005561206874578565	0.001158584765537201	0.0
11	2.317169531074402E-4	0.0	0.008341810311867847	0.001158584765537201	0.0
12	2.317169531074402E-4	0.0	0.008805244218082727	0.0013903017186446412	0.0
13	2.317169531074402E-4	0.0	0.009500395077405048	0.0013903017186446412	0.0
14	2.317169531074402E-4	0.0	0.01042726288983481	0.0013903017186446412	0.0
15	2.317169531074402E-4	0.0	0.01112241374915713	0.0016220186717520815	0.0
16	2.317169531074402E-4	0.0	0.012976149374016652	0.0016220186717520815	0.0
17	2.317169531074402E-4	0.0	0.013207866327124092	0.0016220186717520815	0.0
18	2.317169531074402E-4	0.0	0.013439583280231532	0.0018537356248595216	0.0
19	2.317169531074402E-4	0.0	0.013439583280231532	0.002085452577966962	0.0
20	2.317169531074402E-4	0.0	0.013903017186446412	0.002317169531074402	0.0
21	2.317169531074402E-4	0.0	0.014598168045768732	0.002317169531074402	0.0
22	2.317169531074402E-4	0.0	0.014829884998876173	0.0030123203903967225	0.0
23	2.317169531074402E-4	0.0	0.014829884998876173	0.004402622109041364	0.0
24	2.317169531074402E-4	0.0	0.014829884998876173	0.004866056015256244	0.0
25	2.317169531074402E-4	0.0	0.015061601951983613	0.005561206874578565	0.0
26	2.317169531074402E-4	0.0	0.015061601951983613	0.006024640780793445	0.0
27	2.317169531074402E-4	0.0	0.015061601951983613	0.007414942499438086	0.0
28	4.634339062148804E-4	0.0	0.015061601951983613	0.020159374920347296	0.0
29	4.634339062148804E-4	0.0	0.015061601951983613	0.04286763632487644	0.0
30	4.634339062148804E-4	0.0	0.015061601951983613	0.07438114194748831	0.0
31	4.634339062148804E-4	0.0	0.015061601951983613	0.1378715870989269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCTC	70	2.055458E-10	29.071428	27
CCGCTCT	65	2.9795046E-9	28.461538	28
GGTATCA	445	0.0	26.19101	1
GCTTCGC	85	6.91216E-11	26.117647	22
CGCTCTC	75	1.3686076E-8	24.666666	29
GCCGGCA	85	1.9681465E-9	23.941177	15
GTATCAA	1315	0.0	23.494297	1
TTCGCCG	95	2.8012437E-10	23.368422	24
AGCTTCG	100	5.311449E-10	22.2	21
CGCCGCT	95	7.1140676E-9	21.421053	26
CGGCAGC	105	9.786163E-10	21.142859	17
TGCTCGC	105	9.786163E-10	21.142859	10
CACGGTA	45	0.0038219953	20.555555	2
AGTTACG	45	0.0038219953	20.555555	30
ACGGTAC	45	0.0038219953	20.555555	3
TCGCCGC	100	1.2842065E-8	20.35	25
ACGGGTA	55	5.1359425E-4	20.181818	17
TTCTGCG	55	5.1359425E-4	20.181818	18
AGGTCGC	110	1.7462298E-9	20.181818	35
TATTAGA	215	0.0	19.790697	2
>>END_MODULE