FastQCFastQC Report
Fri 10 Feb 2017
ERR1633523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236106
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67802.87159157327641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53462.264237249371045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25761.091035382412984No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16990.7195920476396195No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.582365547677738No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12910.5467883069468797No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12760.5404352282449408No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9180.3888084165586643No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8580.36339610175090853No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7870.3333248625617308No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7570.32061870515785285No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7390.31299501071552605No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6860.2905474659686751No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.2858885415872532No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6680.28292377152634834No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6410.2714882298628582No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6270.2655586897410485No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6260.2651351511609193No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6170.26132330393975584No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5860.2481936079557487No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5670.2401463749332927No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5640.23887575919290488No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5550.2350639119717415No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5530.23421683481148298No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5450.23082852617044886No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5380.227863756109544No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5180.21939298450695874No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5080.2151575987056661No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4890.2071103656832101No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4780.2024514413017882No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4760.20160436414152966No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4720.19991020982101257No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4710.19948667124088335No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.19567482401971997No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.19313359253894435No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4370.18508635951648836No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4350.18423928235622983No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4290.18169805087545426No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4280.18127451229532499No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4250.1800038965549372No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.17788620365429086No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4200.17788620365429086No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4200.17788620365429086No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4110.1740743564331275No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4080.17280374069273968No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4060.17195666353248118No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3910.16560358483054222No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3850.16306235334976663No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3840.16263881476963737No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3780.16009758328886178No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3710.15713281322795694No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3700.15670927464782766No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3670.15543865890743988No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3620.15332096600679357No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3530.14950911878563017No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3530.14950911878563017No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3480.14739142588498386No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3470.1469678873048546No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3440.1456972715644668No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3430.14527373298433754No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3410.14442665582407901No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3230.13680296138175227No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3200.1355323456413645No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3190.1351088070612352No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3170.13426172990097668No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3170.13426172990097668No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3110.1317204984202011No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3110.1317204984202011No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3070.13002634409968405No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3050.1291792669394255No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3040.12875572835929625No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3040.12875572835929625No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3030.128332189779167No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2990.12663803545864993No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2970.1257909582983914No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2930.12409680397787436No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2920.12367326539774509No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2910.12324972681761581No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2880.12197911107722803No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2870.12155557249709877No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2870.12155557249709877No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA2830.1198614181765817No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2810.11901434101632317No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2810.11901434101632317No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC2790.11816726385606466No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA2670.11308480089451348No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA2640.11181418515412568No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2570.10884941509322082No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2530.10715526077270379No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC2520.10673172219257453No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2510.10630818361244526No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2450.10376695213166968No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2450.10376695213166968No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGC2440.10334341355154042No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2420.10249633639128188No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2400.10164925923102336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCC200.001838975737.05
GAGGTCG303.5908975E-430.83333226
CGAGGTC303.5908975E-430.83333225
AGGTCGC303.5908975E-430.83333227
GGGTACC250.00548761129.618
CGCTCTC1400.029.07142829
CCGCTCT1450.028.06896828
GCCGCTC1450.028.06896827
TCTCTTC1500.027.13333332
TCTCGCG358.8484504E-426.4285720
GCGAGGT358.8484504E-426.4285724
GGTCGCT358.8484504E-426.4285728
CTCTCTT1550.026.25806431
GCTCTCT1550.026.25806430
GGCGAGT951.0913936E-1125.3157919
TCGCCGT901.4188117E-1024.66666625
GAGTCGC1002.0008883E-1124.05000122
TTCGCCG1700.023.94117724
GCAGCTT1550.023.87096819
GCCGGCA1550.023.87096815
Produced by FastQC (version 0.11.5)