FastQCFastQC Report
Fri 10 Feb 2017
ERR1633522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320327
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79142.4706003552619666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63841.9929634404842549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30110.9399769610429343No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA21260.6636967848479834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.5241518822952795No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15580.48637798249913367No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG15560.48575362051903215No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11770.367437025289782No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10620.33153621143394096No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9060.2828359769860174No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8730.2725340043143413No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8700.27159746134418894No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8560.2672269274834778No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8460.2641051175829699No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8240.2572371358018525No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8020.25036915402073506No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7920.24724734412022714No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.2416280862993129No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7520.23476010451819548No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7240.22601903679677332No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7190.22445813184651933No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6960.21727796907535113No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6760.2110343492743353No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6630.20697599640367498No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6560.20479072947331947No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6550.20447854848326866No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6390.199483652642456No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6350.19823492868225284No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6090.19011822294093225No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6090.19011822294093225No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5950.18574768908022116No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5890.18387460313991638No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5750.1795040692792053No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5700.17794316432895135No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.17388481145829104No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5530.1726360874980879No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.17138736353788472No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5390.1682655536373768No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5380.16795337264732602No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5340.16670464868712284No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5280.1648315627468181No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5230.16327065779656413No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5220.16295847680651335No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5210.16264629581646256No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5200.16233411482641177No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5120.15983666690600543No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5110.1595244859159546No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5040.1573392189855991No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5020.1567148570054975No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4930.15390522809504037No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.15234432314478644No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4880.15234432314478644No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4860.15171996116468484No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4700.14672506532387217No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4660.145476341363669No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4580.14297889344326267No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4470.13954490255270394No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4450.13892054057260236No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4240.13236473978153576No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4220.13174037780143416No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4160.1298672918611294No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4060.1267454819606215No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4060.1267454819606215No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA4040.1261211199805199No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4000.12487239602031674No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3920.12237494809991041No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3920.12237494809991041No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3890.12143840512975804No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3830.11956531918945326No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3780.11800441423919933No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3740.11675569027899614No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3690.1151947853287422No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3680.1148826043386914No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3660.11425824235858982No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3660.11425824235858982No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3590.11207297542823427No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC3590.11207297542823427No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3580.11176079443818347No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3570.1114486134481327No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3540.11051207047798031No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3480.10863898453767556No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3470.10832680354762476No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3460.10801462255757399No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3440.1073902605774724No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3390.10582935562721844No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3310.1033319077068121No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3240.10114664077645655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC303.593735E-430.8333329
TTATAGA250.00549049829.64
TATAGAT250.00549049829.65
GTGACAC250.00549049829.624
ACTAGTG405.9269638E-527.758
AGATCTT358.855375E-426.4285728
TTGCTCT358.855375E-426.42857216
AGCTTTA358.855375E-426.42857210
TGCTCTC358.855375E-426.42857217
TAGATCT358.855375E-426.4285727
GATCTTG358.855375E-426.4285729
TTTACTA551.897109E-523.5454543
ACTGCTC1900.023.3684228
TCACGCC400.001928163723.12525
GGAGCTT400.001928163723.1258
GGTCGCT400.001928163723.12528
GTGGGCG400.001928163723.12520
TATGGAG400.001928163723.1255
ATCACGC400.001928163723.12524
AGTGCCT400.001928163723.12513
Produced by FastQC (version 0.11.5)