FastQCFastQC Report
Fri 10 Feb 2017
ERR1633521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319418
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18200.569786298830999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.42326982198874197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6950.21758322949865067No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5330.16686598751479254No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5270.16498757114502No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4980.15590855869111947No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4570.1430727134976739No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT4230.13242835406896292No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4130.12929766011934204No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4010.12554082737979702No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4000.12522775798483493No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT3770.1180271619007069No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3700.1158356761359723No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3490.10926121884176847No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3420.10706973307703387No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG3420.10706973307703387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.10237369215260256No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA3220.10080834517779212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCGT251.2316307E-436.99999616
TGCGGTA507.2977855E-933.336
CCGGCAG453.9963525E-628.77777716
CGCGCGA405.9269143E-527.7532
GCCGGCA405.9269143E-527.7515
CGCGATA405.9269143E-527.7534
TTGCGGT556.2379695E-726.9090935
GGTATCA3500.026.4285721
ATACGGA358.855321E-426.428574
CGCTCTC509.064281E-625.89999829
TCACGGT451.3202915E-424.6666661
CACGGTA451.3202915E-424.6666662
TTCGCCG551.8970872E-523.54545424
CGGAATT400.001928152423.12527
GCGTTAA400.001928152423.12519
CGTTAAT400.001928152423.12520
TCTGGAC400.001928152423.1253
GCAAGCG502.6961873E-422.19999915
GCGATAC502.6961873E-422.19999935
GTATCAA9100.021.9560431
Produced by FastQC (version 0.11.5)