##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319418
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861297735255995	33.0	33.0	33.0	33.0	33.0
2	31.985163641372747	33.0	33.0	33.0	33.0	33.0
3	31.70637221446506	33.0	33.0	33.0	27.0	33.0
4	31.98494762349022	33.0	33.0	33.0	33.0	33.0
5	32.07910324402507	33.0	33.0	33.0	33.0	33.0
6	35.25813823892204	37.0	37.0	37.0	33.0	37.0
7	35.401777608024595	37.0	37.0	37.0	33.0	37.0
8	35.52214026761172	37.0	37.0	37.0	33.0	37.0
9	35.63040592577751	37.0	37.0	37.0	33.0	37.0
10	35.639018464832915	37.0	37.0	37.0	33.0	37.0
11	35.647508906824285	37.0	37.0	37.0	33.0	37.0
12	35.649521943033896	37.0	37.0	37.0	33.0	37.0
13	35.631104070528274	37.0	37.0	37.0	33.0	37.0
14	35.620181079338046	37.0	37.0	37.0	33.0	37.0
15	35.62567544721963	37.0	37.0	37.0	33.0	37.0
16	35.61569166421429	37.0	37.0	37.0	33.0	37.0
17	35.6023674307647	37.0	37.0	37.0	33.0	37.0
18	35.58874891208385	37.0	37.0	37.0	33.0	37.0
19	35.58296965105285	37.0	37.0	37.0	33.0	37.0
20	35.579225341089106	37.0	37.0	37.0	33.0	37.0
21	35.58787857916586	37.0	37.0	37.0	33.0	37.0
22	35.48702953496672	37.0	37.0	37.0	33.0	37.0
23	35.545128953283786	37.0	37.0	37.0	33.0	37.0
24	35.53685452917494	37.0	37.0	37.0	33.0	37.0
25	35.531441559336045	37.0	37.0	37.0	33.0	37.0
26	35.435360562022176	37.0	37.0	37.0	33.0	37.0
27	35.452585640132995	37.0	37.0	37.0	33.0	37.0
28	35.46416607705264	37.0	37.0	37.0	33.0	37.0
29	35.473201259791246	37.0	37.0	37.0	33.0	37.0
30	35.47036798176684	37.0	37.0	37.0	33.0	37.0
31	35.452795396627614	37.0	37.0	37.0	33.0	37.0
32	35.44190684307083	37.0	37.0	37.0	33.0	37.0
33	35.43741742794708	37.0	37.0	37.0	33.0	37.0
34	35.412243517898176	37.0	37.0	37.0	33.0	37.0
35	35.36137913329868	37.0	37.0	37.0	33.0	37.0
36	35.37976256817086	37.0	37.0	37.0	33.0	37.0
37	35.38166289939828	37.0	37.0	37.0	33.0	37.0
38	35.35762856194704	37.0	37.0	37.0	33.0	37.0
39	35.25958461952676	37.0	37.0	37.0	33.0	37.0
40	35.03543006342786	37.0	37.0	37.0	27.0	37.0
41	35.21188223581639	37.0	37.0	37.0	33.0	37.0
42	35.24137963420972	37.0	37.0	37.0	33.0	37.0
43	34.907747841386524	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	21.0
17	10.0
18	11.0
19	12.0
20	25.0
21	63.0
22	144.0
23	347.0
24	728.0
25	1391.0
26	2126.0
27	3158.0
28	4440.0
29	5843.0
30	7804.0
31	10172.0
32	12870.0
33	16886.0
34	24420.0
35	50000.0
36	178934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38282125615964	21.63841737159459	13.850816171912667	24.127945200333105
2	15.850390397535518	21.780863946302336	39.15840685246292	23.21033880369923
3	17.207546224696166	27.957410039509355	30.37587111559148	24.45917262020299
4	11.952050291467607	18.30047148250881	40.79325523295494	28.954222993068647
5	13.070021100877222	38.03855762668353	35.7935369954104	13.097884277028845
6	28.3806798614981	41.142327608337666	17.650539418567522	12.826453111596717
7	25.710197922471494	32.85444151550633	24.04466874127319	17.390691820748987
8	23.798283127438026	36.31229298286258	20.757127024776313	19.132296864923077
9	25.463499239241372	15.346348671646556	20.81535793223926	38.37479415687281
10	14.79941643864779	28.37316619601901	34.10922365051437	22.718193714818828
11	33.23857766312481	24.324239710974336	24.532743928019084	17.904438697881773
12	22.63648260273372	26.51791696147368	30.30824812627967	20.537352309512926
13	27.237037361701592	22.99369478238546	26.464695164330127	23.304572691582816
14	21.403928394767984	21.500353768416307	28.581044274273836	28.514673562541876
15	23.652705858780656	29.1486390873401	25.32261801150843	21.87603704237081
16	22.239823679316757	29.040943215473142	25.741191792572742	22.97804131263736
17	21.77115879505851	28.226649719176756	26.71139384756025	23.290797638204484
18	22.125240280760632	27.769255333137146	28.56351238815596	21.541991997946262
19	24.243467807074115	26.876694488100235	28.129285137343548	20.75055256748211
20	23.23507128590123	26.707950084215664	28.604524478895993	21.45245415098711
21	22.420464720209882	27.595501818933183	28.50559455008797	21.47843891076896
22	22.09925552097878	27.490623571620887	28.154643758335475	22.255477149064863
23	21.95555666869118	27.413295431065247	28.919472290227855	21.711675610015714
24	22.33781439993989	27.963045288618677	27.02164561796768	22.677494693473754
25	22.220413376829107	27.747966614279722	28.345303019867384	21.686316989023787
26	22.33624905296508	27.81058049327214	28.4376584913812	21.41551196238158
27	22.334370636595306	27.744522850935137	27.456812076964983	22.464294435504573
28	21.76427126836935	27.35318610723253	28.91008020837899	21.972462416019134
29	23.52841730898071	27.200095173096067	27.65655035095079	21.61493716697243
30	22.94078605463687	26.72923880307309	28.419500466473398	21.91047467581664
31	22.377261143705113	27.167222886625048	28.19440357149566	22.26111239817418
32	21.2320532969338	27.073928206926347	28.804575822276767	22.88944267386309
33	21.487830992617823	27.24486409657565	29.071624016179427	22.195680894627102
34	22.247650414190808	27.210426463129817	28.72849995930098	21.813423163378395
35	22.443005716647153	27.831243073339635	27.830930003944676	21.89482120606854
36	23.239141188035738	27.288067673080413	27.57984834918508	21.892942789698765
37	22.449267104546394	26.779329906267023	27.865993776180424	22.905409213006156
38	22.453963145470826	27.35318610723253	27.86035852707111	22.332492220225536
39	21.80528335910938	27.51566912321785	28.326831925564623	22.352215592108145
40	22.077966802121356	28.04319105372897	28.2394855643702	21.639356579779474
41	21.8653926829421	26.88420815357932	28.319005190690564	22.93139397278801
42	21.69570907087265	28.186889906016567	27.873507441659516	22.243893581451264
43	22.57011189100176	26.828481801276073	27.982455591106326	22.618950716615846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	25.5
2	30.0
3	122.0
4	214.0
5	214.0
6	230.0
7	246.0
8	249.0
9	252.0
10	346.0
11	440.0
12	440.0
13	678.5
14	917.0
15	1693.5
16	2470.0
17	2798.0
18	3126.0
19	3126.0
20	2997.0
21	2868.0
22	3128.5
23	3389.0
24	4198.0
25	5007.0
26	5007.0
27	6367.0
28	7727.0
29	8727.5
30	9728.0
31	11525.5
32	13323.0
33	13323.0
34	16176.5
35	19030.0
36	20411.5
37	21793.0
38	22935.0
39	24077.0
40	24077.0
41	24972.5
42	25868.0
43	26231.5
44	26595.0
45	25725.5
46	24856.0
47	24856.0
48	23561.0
49	22266.0
50	22155.0
51	22044.0
52	20715.5
53	19387.0
54	19387.0
55	18792.0
56	18197.0
57	15575.0
58	12953.0
59	11759.0
60	10565.0
61	10565.0
62	9244.0
63	7923.0
64	6356.0
65	4789.0
66	4005.5
67	3222.0
68	3222.0
69	2673.5
70	2125.0
71	1791.0
72	1457.0
73	1264.0
74	1071.0
75	1071.0
76	889.0
77	707.0
78	545.5
79	384.0
80	259.5
81	135.0
82	135.0
83	116.0
84	97.0
85	80.5
86	64.0
87	51.0
88	38.0
89	38.0
90	25.5
91	13.0
92	7.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	319418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.49088196778701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.45421702758073	53.50673601768402
2	6.38513304162676	7.724846583393225
3	1.655172134573325	3.003684666865352
4	0.8035518413264572	1.9443023835470654
5	0.49031869378965387	1.4829905116314728
6	0.3173186553402331	1.1516931195817757
7	0.25187225997466356	1.0665182604360997
8	0.1921183793276174	0.9297128166199549
9	0.16796687630515922	0.9144418040165939
>10	1.0727685603491275	12.87536061966413
>50	0.12972918511819675	5.426801622662357
>100	0.07720724782338025	8.407555256748081
>500	0.0015756581188444948	0.5575807533383548
>1k	0.0010504387458963298	1.007775583811545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1820	0.569786298830999	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1352	0.42326982198874197	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	695	0.21758322949865067	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	533	0.16686598751479254	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	527	0.16498757114502	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	498	0.15590855869111947	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	457	0.1430727134976739	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	423	0.13242835406896292	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	413	0.12929766011934204	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	401	0.12554082737979702	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	400	0.12522775798483493	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	377	0.1180271619007069	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	370	0.1158356761359723	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	349	0.10926121884176847	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	342	0.10706973307703387	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	342	0.10706973307703387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	327	0.10237369215260256	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	322	0.10080834517779212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.130693949620873E-4	0.0	0.0
2	0.0	0.0	3.130693949620873E-4	0.0	0.0
3	0.0	0.0	6.261387899241746E-4	0.0	0.0
4	0.0	0.0	6.261387899241746E-4	0.0	0.0
5	0.0	0.0	9.392081848862619E-4	0.0	0.0
6	0.0	0.0	0.0015653469748104364	0.0	0.0
7	0.0	0.0	0.00344376334458296	0.0	0.0
8	0.0	0.0	0.00344376334458296	0.0	0.0
9	0.0	0.0	0.004069902134507135	0.0012522775798483492	0.0
10	6.261387899241746E-4	0.0	0.0046960409244313096	0.0015653469748104364	0.0
11	6.261387899241746E-4	0.0	0.006574457294203833	0.0015653469748104364	0.0
12	6.261387899241746E-4	0.0	0.00688752668916592	0.0018784163697725237	0.0
13	6.261387899241746E-4	0.0	0.007200596084128008	0.0018784163697725237	0.0
14	6.261387899241746E-4	0.0	0.007513665479090095	0.0018784163697725237	0.0
15	6.261387899241746E-4	0.0	0.00813980426901427	0.0018784163697725237	0.0
16	6.261387899241746E-4	0.0	0.010018220638786794	0.002191485764734611	0.0
17	6.261387899241746E-4	0.0	0.010018220638786794	0.002191485764734611	0.0
18	6.261387899241746E-4	0.0	0.010644359428710969	0.002191485764734611	0.0
19	6.261387899241746E-4	0.0	0.010644359428710969	0.0025045551596966985	0.0
20	6.261387899241746E-4	0.0	0.010644359428710969	0.0031306939496208728	0.0
21	6.261387899241746E-4	0.0	0.011270498218635143	0.0037568327395450475	0.0
22	6.261387899241746E-4	0.0	0.011270498218635143	0.004069902134507135	0.0
23	6.261387899241746E-4	0.0	0.011270498218635143	0.004382971529469222	0.0
24	6.261387899241746E-4	0.0	0.011270498218635143	0.005009110319393397	0.0
25	6.261387899241746E-4	0.0	0.011270498218635143	0.005322179714355484	0.0
26	6.261387899241746E-4	0.0	0.011270498218635143	0.005635249109317572	0.0
27	6.261387899241746E-4	0.0	0.011270498218635143	0.00813980426901427	0.0
28	6.261387899241746E-4	0.0	0.011270498218635143	0.01941030248764941	0.0
29	6.261387899241746E-4	0.0	0.011270498218635143	0.04132516013499552	0.0
30	6.261387899241746E-4	0.0	0.011270498218635143	0.06856219749669712	0.0
31	6.261387899241746E-4	0.0	0.011270498218635143	0.12460161919491074	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGCGT	25	1.2316307E-4	36.999996	16
TGCGGTA	50	7.2977855E-9	33.3	36
CCGGCAG	45	3.9963525E-6	28.777777	16
CGCGCGA	40	5.9269143E-5	27.75	32
GCCGGCA	40	5.9269143E-5	27.75	15
CGCGATA	40	5.9269143E-5	27.75	34
TTGCGGT	55	6.2379695E-7	26.90909	35
GGTATCA	350	0.0	26.428572	1
ATACGGA	35	8.855321E-4	26.42857	4
CGCTCTC	50	9.064281E-6	25.899998	29
TCACGGT	45	1.3202915E-4	24.666666	1
CACGGTA	45	1.3202915E-4	24.666666	2
TTCGCCG	55	1.8970872E-5	23.545454	24
CGGAATT	40	0.0019281524	23.125	27
GCGTTAA	40	0.0019281524	23.125	19
CGTTAAT	40	0.0019281524	23.125	20
TCTGGAC	40	0.0019281524	23.125	3
GCAAGCG	50	2.6961873E-4	22.199999	15
GCGATAC	50	2.6961873E-4	22.199999	35
GTATCAA	910	0.0	21.956043	1
>>END_MODULE