FastQCFastQC Report
Fri 10 Feb 2017
ERR1633520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390436
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110332.8258152424469056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88462.265672222848303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43351.1102972062002479No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25310.6482496491102255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.5568134085996168No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21040.5388847339896936No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG18650.4776711163929556No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17710.45359546763105857No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15560.3985288241862943No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15460.3959675849563053No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14790.3788072821153787No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11730.3004333616777142No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11470.29377413967974264No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10760.2755893411468205No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.2727719779938325No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10520.2694423669948468No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10370.26560050814986325No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10370.26560050814986325No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10300.2638076406888709No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9890.2533065598459159No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9430.2415248593879663No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9210.23589013308199042No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9100.23307276992900247No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8850.22666967185402984No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8750.22410843262404084No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8740.2238523087010419No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8310.21283898001208906No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8310.21283898001208906No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8190.20976549293610222No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8010.20515526232212195No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC7990.20464301447612412No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7930.2031062709381307No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7900.202337899169134No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7880.20182565132313618No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.1967031728631581No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7670.1964470489401592No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7390.1892755790961899No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7320.18748271163519759No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.18389697671321292No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7120.18236023317521952No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7090.1815918614062228No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7080.18133573748322387No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7010.17954287002223157No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6830.1749326394082513No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6820.17467651548525237No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6720.17211527625526335No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6620.1695540370252743No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.16724892171828418No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6480.16596830210328964No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6480.16596830210328964No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6440.16494380641129405No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6430.16468768248829516No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6360.1628948150273028No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6310.1616141954123083No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6250.16007745187431485No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6230.15956520402831706No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6140.1572600887213269No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6090.15597946910633242No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6070.1554672212603346No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6060.1552110973373357No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6060.1552110973373357No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6030.154442725568339No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6010.15393047772234117No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6010.15393047772234117No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5930.15188148633834994No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5860.15008861887735764No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5820.149064123185362No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5590.1431732729563872No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5470.14009978588040037No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG5410.13856304234240696No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5400.13830691841940806No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5360.13728242272741242No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5310.1360018031124179No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5200.13318443995942997No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5120.13113544857543874No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5090.13036707680644202No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5040.1290864571914475No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4950.1267813418844574No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4850.12422010265446834No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4830.12370785480847055No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4810.12319560696247273No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4700.12037824380948478No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4690.12012211988648587No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4670.11960987204048808No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4630.11858537634849245No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA4610.11807312850249466No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4610.11807312850249466No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4590.11756088065649684No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4570.11704863281049903No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4550.11653638496450124No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4540.11628026104150231No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA4480.11474351750350889No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4460.11423126965751108No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4450.11397514573451219No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4400.11269452611951766No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4400.11269452611951766No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4360.11167003042752205No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4240.10859654335153521No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4210.1078281715825385No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC4160.10654755196754398No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4060.10398631273755493No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA4050.10373018881455603No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4010.10270569312256042No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG3990.10219344527656261No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3970.1016811974305648No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3960.1014250735075659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC200.001840447537.02
TTTCAAC251.2321326E-437.02
GTTTCAA250.00549195329.61
CTCTTTT250.00549195329.636
GCCGCTC3200.026.01562727
GTATTAT509.071022E-625.9000021
TATTATA509.071022E-625.9000022
ATTATAG509.071022E-625.9000023
CCGCTCT3150.025.8412728
CGCTCTC3250.025.04615429
GGTCGCT451.3210265E-424.66666828
CACTGTG451.3210265E-424.6666687
GCTCTCG451.3210265E-424.66666818
TCGCTTC451.3210265E-424.66666830
TCTCGCG451.3210265E-424.66666820
ACTGTGC451.3210265E-424.6666688
CTGTGCA451.3210265E-424.6666689
GCTCTCT3300.024.66666630
GGCAGCT3550.024.49295818
GGTATCA17150.024.1632651
Produced by FastQC (version 0.11.5)