FastQCFastQC Report
Fri 10 Feb 2017
ERR1633519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434016
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122072.812569121875691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86271.9877147386271474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47661.0981161984811618No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA27240.6276266312762663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.5439891616898916No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23370.5384594116345941No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG23240.5354641303546414No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18960.43685025436850256No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17450.40205891027058915No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13390.30851397183513973No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13120.30229300302293005No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.2896206591462066No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12520.28846862788468625No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11970.27579628400796286No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11920.27464425274644255No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11340.2612806901128069No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10940.25206444002064443No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG10640.24515225245152253No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10420.24008331490083315No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10330.2380096586300966No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10030.23109747106097472No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10030.23109747106097472No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9990.23017584605175848No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9810.22602853351028535No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9780.22533731475337318No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9710.2237244709872447No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9650.22234203347342033No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9210.2122041583720416No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9170.21128253336282535No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8710.20068384575683845No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8590.1979189707291897No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8500.19584531445845316No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8410.19377165818771658No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7780.17925606429256066No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7730.17810403303104033No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7730.17810403303104033No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7520.173265501732655No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7370.16980940794809407No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7360.16957900169579002No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.16727493917274938No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7230.1665837204158372No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7180.1654316891543169No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6760.15575462655754627No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6730.15506340780063407No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6730.15506340780063407No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.15275934527759347No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6610.15229853277298533No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.15137690776376908No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6550.15091609525916097No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6550.15091609525916097No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6420.14792081397920814No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6390.14722959522229595No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6300.1451559389515594No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6220.14331268893312687No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6180.14239106392391063No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6030.13893497013934972No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6000.13824375138243752No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5900.1359396888593969No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5820.1340964388409644No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5820.1340964388409644No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5700.13133156381331562No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5620.12948831379488313No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5560.12810587628105877No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5510.12695384501953844No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5470.12603222001032222No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5470.12603222001032222No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5380.12395856373958565No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5280.12165450121654502No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC5220.12027206370272064No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5200.1198112511981125No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5040.1161247511612475No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5010.11543353240433532No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4920.11335987613359876No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4870.11220784487207845No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4860.1119774386197744No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4860.1119774386197744No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4860.1119774386197744No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4820.11105581361055815No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4770.10990378234903783No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4740.10921256359212564No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4650.10713890732138907No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4650.10713890732138907No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4530.10437403229374032No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4510.10391321978913219No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4440.10230037602300375No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4420.10183956351839563No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4390.10114834476148343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTG200.001840674337.017
ACAACGC303.5958196E-430.8333343
AGGAGCC250.00549262329.5999987
TTTCCTA250.00549262329.59999814
GTGTCCA250.00549262329.59999821
AGTGTCC250.00549262329.59999820
TTAACGG405.931744E-527.7535
CCAAGCA405.931744E-527.7536
GGTATCA15200.027.3848691
TGTTTAA358.8604656E-426.4285732
GTACCGG358.8604656E-426.428576
GTATTAT358.8604656E-426.428571
TCCTGCT358.8604656E-426.4285737
AGCGGTC358.8604656E-426.4285719
GGAAATT358.8604656E-426.4285719
ACCCGGT358.8604656E-426.4285714
GCTCGGA509.074078E-625.89999811
TGAGCGT1000.025.89999835
CGGAGGT509.074078E-625.89999814
CTAGTGC951.0913936E-1125.315799
Produced by FastQC (version 0.11.5)