FastQCFastQC Report
Fri 10 Feb 2017
ERR1633518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720518
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57410.7967878665071518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.5843018495027189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22660.3144959598511071No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12750.17695602330545523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.16821231391859745No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9750.13531931193946578No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA9320.12935138331034063No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8510.11810947124152346No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7650.10617361398327316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA451.3225424E-424.66666822
GTATCAA25800.023.5910851
GGTATCA9100.023.379121
CGATTAC400.001930491623.12527
ACGTAGA400.001930491623.12526
GGACGTA855.1739335E-821.76470627
CCGCTCT603.7238024E-521.58333428
CGGGCGA450.003824442420.55555717
GTACTAG450.003824442420.5555571
TGCCGAG450.003824442420.55555711
TTGCCGA555.1405333E-420.18181810
GCCGCTC656.897562E-519.92307727
CGCTCTC656.897562E-519.92307729
GTCTTAC759.257905E-619.7333341
GACGTAT851.2439887E-619.58823628
AAACACG851.2439887E-619.58823615
CGGCGTG609.2322554E-418.536
ACACGAA902.1499472E-618.517
GCCGGCA609.2322554E-418.515
CACGAGC500.00703239618.535
Produced by FastQC (version 0.11.5)